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Preparation method of edible and medicinal hericium erinaceus dry powder

A technology of Hericium erinaceus powder and Hericium erinaceus is applied in the field of preparation of edible and medicinal Hericium erinaceus dry powder, and can solve the problems of low extraction rate, reduced extraction rate, inability to obtain high extraction rate Hericium erinaceus polysaccharide, etc. The effect of improving enzymatic hydrolysis activity and improving purity

Pending Publication Date: 2020-07-31
安徽斯普瑞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction methods of Hericium erinaceus polysaccharides mainly adopt water extraction, acid extraction, alkali extraction, and enzyme extraction. Among them, water extraction has low cost input, but the extraction time is long and the extraction rate is not high; acid extraction and alkali The extraction method is easy to break the glycosidic bond, and the extraction rate of some polysaccharides is reduced due to hydrolysis, and the impurity content is not low; relatively speaking, the extraction conditions of the enzyme extraction method are relatively mild, but the enzyme extraction method alone cannot obtain high extraction rates. High-purity Hericium erinaceus polysaccharide products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Activation of the slope of the strain: In a sterile environment, insert the parent species into the slope of the test tube, and place it in an incubator at a constant temperature of 25°C for 15 days until the slope of the test tube is covered with mycelia to obtain the activated strain;

[0035] (2) Expansion of bacterial strains: in a sterile environment, insert activated bacterial strains into the primary seed tank, and cultivate at a constant temperature of 25°C for 7 days to obtain the primary seed tank bacterial strains;

[0036] (3) Fermentation tank cultivation: insert the first-level seed tank strains into the fermenter, feed sterile air and carry out constant temperature fermentation at 25°C for 7 days to obtain liquid strains;

[0037] (4) Solid culture and fruiting: put liquid bacteria into the culture bottle, place it in a culture room with a temperature of 25°C and a humidity of 55% for cultivation, increase the temperature difference and give scattered ...

Embodiment 2

[0043] The difference between embodiment 2 and embodiment 1 is that the activator magnesium sulfate is replaced by calcium dihydrogen phosphate.

[0044] (1) Activation of the slope of the strain: In a sterile environment, insert the parent species into the slope of the test tube, and place it in an incubator at a constant temperature of 25°C for 15 days until the slope of the test tube is covered with mycelia to obtain the activated strain;

[0045] (2) Expansion of bacterial strains: in a sterile environment, insert activated bacterial strains into the primary seed tank, and cultivate at a constant temperature of 25°C for 7 days to obtain the primary seed tank bacterial strains;

[0046] (3) Fermentation tank cultivation: insert the first-level seed tank strains into the fermenter, feed sterile air and carry out constant temperature fermentation at 25°C for 7 days to obtain liquid strains;

[0047] (4) Solid culture and fruiting: put liquid bacteria into the culture bottle, ...

Embodiment 3

[0053]The difference between embodiment 3 and embodiment 1 is that the activator is replaced by acetamide cyclopropyl lactate.

[0054] Preparation of acetamide cyclopropyllactate: firstly dissolve 10mol lactic acid, 10mol 1-cyclopropylethylamine hydrochloride, 11mol EDCI and 11mol HOBt in water to make a solution, then mix these four solutions and heat to 65 The reaction was carried out at ℃ for 3 hours, and the reaction solution was concentrated and then column chromatographed to obtain cyclopropyllactate acetamide. Elemental analysis: theoretical value C, 61.12; H, 9.62; N, 8.91; O, 20.35. Measured value C, 61.13; H, 9.61; N, 8.90; O, 20.36.

[0055] (1) Activation of the slope of the strain: In a sterile environment, insert the parent species into the slope of the test tube, and place it in an incubator at a constant temperature of 25°C for 15 days until the slope of the test tube is covered with mycelia to obtain the activated strain;

[0056] (2) Expansion of bacterial ...

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Abstract

The invention discloses a preparation method of edible and medicinal hericium erinaceus dry powder, and relates to the technical field of edible and medicinal product processing. Hericium erinaceus isheated and pressurized before extraction to soften and destroy the cell wall structure, and therefore leaching of polysaccharide components during subsequent extraction is facilitated; enzymolysis activity of the compound enzyme is improved by adding an activating agent, so that the extraction rate of hericium erinaceus polysaccharide is improved while the extraction time is shortened; and an alcohol precipitation method is combined with DEAE cellulose column chromatography to separate and purify the extract, so that the purity of hericium erinaceus polysaccharide in the product is improved.

Description

Technical field: [0001] The invention relates to the technical field of food and drug product processing, in particular to a preparation method of Hericium erinaceus dry powder for food and medicine. Background technique: [0002] Hericium erinaceus, also known as Hericium erinaceus, got its name only because of its resemblance to Hericium erinaceus. Hericium erinaceus is an incomparably delicious mountain delicacy, with fresh and tender meat, mellow and delicious, known as "vegetarian meat". As a treasure in the kingdom of edible fungi, Hericium erinaceus is a fungus with both medicinal and edible uses, especially its medicinal value in medicine, which is favored by consumers. It has the effects of strengthening the spleen and stomach, calming the nerves, and fighting cancer. [0003] The main component of Hericium erinaceus is polysaccharide, which has the functions of aiding digestion and benefiting the five internal organs. The polysaccharide extracted from the fruiting...

Claims

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Application Information

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IPC IPC(8): A23L33/125A23L31/00A01G18/00A01G18/40C08B37/02A61K36/07A61K9/14
CPCA23L33/125A23L31/00A01G18/00A01G18/40C08B37/0003C08B37/0024A61K36/07A61K9/14A23V2002/00A61K2236/15A61K2236/19A61K2236/333A61K2236/55A23V2200/30A23V2250/208A23V2250/51
Inventor 苏振泉
Owner 安徽斯普瑞生物科技有限公司
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