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Microbacterium waters mb338 and its application

A technology of microbacteria and waters, applied in the field of microorganisms, can solve the problems of pollution, cellulose resources that cannot be processed and utilized in a timely and effective manner, and aggravate the air, so as to achieve the effects of alleviating environmental pollution, reducing salinity stress, and increasing production

Active Publication Date: 2021-09-28
HEBEI MONBAND WATER SOLUBLE FERTILIZER CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] my country is a large agricultural country. The Northeast and Northern regions are important grain storage areas in my country, and are the areas with the largest reserves of plant lignocellulose. After crops are harvested, there will be a large amount of crop straw waiting to be processed. The traditional processing methods are mostly incineration and straw returning to the field. , due to the severe smog in the northern region, the burning of straw will aggravate the problem of air pollution. Therefore, the government has regulated the burning of straw, and a large amount of straw can only be decomposed by returning to the field. The decomposition rate of straw and the cold conditions in the Northeast and northern regions make it impossible for a large amount of cellulose resources to be processed and utilized in a timely and effective manner

Method used

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  • Microbacterium waters mb338 and its application
  • Microbacterium waters mb338 and its application
  • Microbacterium waters mb338 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] 1. Screen microbial strains with the ability to degrade silicon:

[0055] 1.1 Collection of soil samples

[0056] Select representative soils from different provinces across the country for collection, such as sandy soil, clay soil, black soil, etc. The samples can come from different areas such as farmland, pasture land, forest soil, etc., especially for corn, rice, and wheat fields. The soil is sampled, and 15-20 grams of samples are collected at each point, and the source (province, county), year and month of collection, and the source of the soil (plant, sand, or other) are marked at the same time, and they are stored in 80% glycerin tubes. -80°C refrigerator, a total of 100 soil samples from various provinces and cities across the country were collected.

[0057] 1.2 Screening of microbial strains that degrade silicon

[0058] (1) Shake 0.50g soil sample in 15mL water, and dilute the mixture to 10 5 、10 4 、10 3 After that, 100 μL of each was spread on the R2A ...

Embodiment 2

[0085] Determination of the ability of bacterial strain MB338 to degrade silicon:

[0086] The bacterial strain MB338 grown on the R2A medium for 2 days was inoculated in a silicon-containing mineral medium, and Bacillus subtilis (strain 92068) and silicate bacteria (strain 3016) were set as controls. After culturing for 1-2 days, the determination Solve the diameter of the silicon ring, in mm.

[0087] Desiliconization ability = the diameter of the desilicon circle in millimeters + X;

[0088] X is a weighting coefficient, which is -2, -1, 0, 1, 2 according to the degree of transparency of the strain's silicon circle. (Note: X is a weighting coefficient, according to the degree of transparency of the strain's hydrolysis zone, correspondingly: -1, 0, 1, 2. The number 2 means that the hydrolysis zone is completely transparent; the number 1 means that the dissolution zone is translucent; 0 means that the dissolution zone It is opaque, but there are traces of hydrolysis on the ...

Embodiment 3

[0093] Determination of protein degradation ability of bacterial strain MB338:

[0094] Bacterial strain MB338 grown on the R2A medium for 2 days was inoculated in a protein-containing medium (yeast extract 0.50 g, peptone 0.50 g, tryptone 0.50 g, glucose 0.50 g, soluble starch 0.50 g, dipotassium hydrogen phosphate 0.30 g, sodium pyruvate 0.30g, magnesium sulfate heptahydrate 0.05g, skimmed milk powder 5g, agar powder 15.0g, water 1000mL), set Bacillus subtilis (bacterial strain 92068) as a control, after cultivating for 1-2d, measure the proteolytic circle diameter, in mm.

[0095] Proteolytic ability = the diameter of the proteolytic circle in millimeters + X

[0096] X is a weighting coefficient, which is -2, -1, 0, 1, 2 according to the degree of transparency of the proteosphere of the strain.

[0097]The assay result of bacterial strain MB338 degradation protein ability is as table 2 and Figure 4 Shown:

[0098] The ability of bacterial strains to degrade protein on...

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Abstract

The invention provides microbacteria MB338 and its application, and relates to the technical field of microbes. The preservation number of Exiguobacterium undae MB338 in the General Microorganism Center of China Committee for Culture Collection of Microorganisms is: CGMCC No.19489. Strain MB338 has the functions of degrading silicon, protein, cellulose and starch, and promoting decomposition.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a microbacterium in waters MB338 and its application. Background technique [0002] Mineral nutrition can affect the defense ability of crops against diseases, and is considered to be one of the important factors in the regulation of crop diseases. The regulation of mineral nutrients can reduce the occurrence of many crop diseases. Silicon has been recognized by the international soil science community as the fourth plant nutrient element after nitrogen, phosphorus, and potassium, and it is an essential nutrient for grasses and root crops. Silicon is the second largest element in the earth's crust, with a content of 27.6% in the earth's crust, accounting for about a quarter of the soil composition, but the vast majority of silicon exists in the form of silicate crystals or precipitation, which is ineffective and cannot be The direct absorption and utilization of plants must go ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F17/20C05F11/00C05G3/80C12R1/01
CPCC12N1/20C05F11/00C12R2001/01C12N1/205Y02W30/40C05F11/08
Inventor 郭一韬郭兴龙吴广利孟国明苑莹康耀卫
Owner HEBEI MONBAND WATER SOLUBLE FERTILIZER CO LTD
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