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A Strain of Streptomyces oryzae and Its Application in Controlling Wheat Root Rot and Stem Rot

A technology of Streptomyces oryzae and biocontrol agents, applied in application, microorganism-based methods, bacteria, etc., can solve problems such as enhanced drug resistance of pathogenic bacteria, ineffective chemical control methods, and environmental pollution.

Active Publication Date: 2022-02-01
CHINA UNIV OF GEOSCIENCES (BEIJING)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the disease is a soil-borne disease, it is difficult to use chemical control methods to be effective; and the use of chemical agents will also cause the enhancement of pathogenic bacteria resistance and the resulting environmental pollution

Method used

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  • A Strain of Streptomyces oryzae and Its Application in Controlling Wheat Root Rot and Stem Rot
  • A Strain of Streptomyces oryzae and Its Application in Controlling Wheat Root Rot and Stem Rot
  • A Strain of Streptomyces oryzae and Its Application in Controlling Wheat Root Rot and Stem Rot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1, the screening of antagonizing Umbilaria graminis, Fusarium graminearum and Pseudofusarium graminearum

[0017] 1. Isolation and storage of actinomycetes

[0018] Collect a 5-10cm sample of the sediment surface layer of the Qilihai Lagoon in Beidaihe, weigh 10g of the sediment, put it into a triangular flask filled with 90ml of sterile water, shake it for 30min at 30°C and 150rpm, and obtain a dilution of 10 -1 bacterial suspension. Take 1ml of the above-mentioned sediment bacterial suspension, add it to a test tube filled with 9ml sterile water, vortex and mix well, and obtain a dilution of 10 -2 Bacterial suspension; do 10-fold gradient dilution to 10 by the above method -4 . Take 10 respectively -1 to 10 -4 20 μl of the bacterial suspension was evenly spread on Gaoshi No. 1 medium plate (soluble starch 20g / L, KNO 3 1g / L, NaCl 0.5g / L, K 2 HPO 4 ·3H 2 O 0.5g / L, MgSO 4 ·7H 2 O0.5g / L, FeSO 4 ·7H 2 O 0.5g / L, agar 15g / L, pH 7.2~7.4; add K with ...

Embodiment 2

[0021] Physiological and biochemical characteristics and species identification of embodiment 2, Q1C-5 bacterial strain

[0022] 1. Physiological and biochemical characteristics of the strain

[0023] Colony color and shape observation method. Take the preserved Q1C-5 strain, and use the plate streaking method to streak continuously on the Gaoshi No. 1 plate and the PSA plate respectively, and culture the streaked plate upside down in a 30°C incubator until a single colony appears.

[0024] Strain enzyme production detection method. Lipase detection medium: beef extract 3g / L, peptone 10g / L, tributyrin 6ml / L, NaCl 10g / L, agar 20g / L. Cellulose detection medium; carboxymethylcellulose 5g / L, NaNO 3 1g / L, K 2 HPO 4 2g / L, KCl 1g / L, MgSO 4 .7H 2 O 0.5g / L, yeast powder 2g / L, glucose 1g / L, NaCl 10g / L, agar 20g / L. Protease detection medium: skimmed milk powder 15g / L, yeast powder 2g / L, NaCl 10g / L, agar 20g / L. Amylase detection medium: starch 5g / L, yeast powder 2g / L, (NH 4 ) ...

Embodiment 3

[0034] Embodiment 3, Streptomycesmisionensis Q1C-5CGMCC NO.18008 on wheat root rot Inhibitory Physiological Characteristics of Helminthroides, Fusarium graminearum and Pseudomonas graminearum

[0035] 1. Physiological characteristics of Q1C-5 inhibiting wheat pathogenic fungi in confrontation culture

[0036] According to the method described in the second step in Example 1, Streptomyces misionensis Q1C-5 was cultured in confrontation with Umbilium solani, Fusarium graminearum and Fusarium graminearum respectively, and then cultivated at 30°C until When the leading edge mycelium of pathogenic fungi grows to 3cm on the side not connected with actinomycetes, measure the colony radius of the pathogenic fungi connected with actinomycetes at this moment, and according to the formula: inhibition rate=[(3cm-pathogenic fungi colony growth radius) / 3cm]×100%, calculate the inhibitory rate of actinomycetes Q1C-5 to above three strains of pathogenic fungi; , and then observed under a...

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Abstract

The invention discloses an actinomycete capable of antagonizing the pathogenic bacteria of wheat root rot and stem-base rot and its application. The actinomycete is Streptomyces misionensis Q1C‑5 CGMCC NO.18008. It has an antagonistic effect on Bipolaris sorokiniana, Fusarium graminearum and Fusarium pseudograminearum, and can effectively inhibit the hyphal elongation and spore germination of pathogenic fungi. It is used for the control of wheat root rot caused by Helmintheroides rot, and wheat stem rot caused by Fusarium graminearum and Fusarium graminearum.

Description

technical field [0001] The invention relates to a strain of Streptomyces oryzae which has antagonism to wheat root rot, Fusarium graminearum and pseudofusarium graminearum and its application in preventing and treating wheat root rot and wheat stem rot. Background technique [0002] With the improvement of people's living standards, people pay more and more attention to the green agricultural development model, that is, the agricultural development model that protects the environment and ensures that agricultural products are green and pollution-free while promoting agricultural development. However, in the actual production process in my country at present, due to the continuous cropping of crops, some pathogenic bacteria break out year after year, forming diseased soil; in addition, due to the long-term application of chemical fertilizers and pesticides, the organic matter in the soil is reduced, and the soil microbial ecology is destroyed, making soil-borne diseases year a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/28A01P3/00C05F11/08C12R1/465
CPCC12N1/20A01N63/00C05F11/08C12R2001/465C12N1/205
Inventor 魏士平边佳琪
Owner CHINA UNIV OF GEOSCIENCES (BEIJING)
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