Intelligent macromolecular amphoteric lipid material as well as synthesis method and application thereof in complex drug delivery preparation

An intelligent polymer and lipid material technology, applied in the field of intelligent liposomes, can solve the problems of inability to release drugs and function, and improve the effect of treating diseases

Inactive Publication Date: 2020-08-11
XIAN LIBANG PHARMA
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although we have successfully encapsulated various drugs in liposomes and sent them to lesions and diseased cells, most conventional liposomes cannot respond to changes in the microenvironment at the cellular level, and liposomes sta

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Intelligent macromolecular amphoteric lipid material as well as synthesis method and application thereof in complex drug delivery preparation
  • Intelligent macromolecular amphoteric lipid material as well as synthesis method and application thereof in complex drug delivery preparation
  • Intelligent macromolecular amphoteric lipid material as well as synthesis method and application thereof in complex drug delivery preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1.1 Liposome preparation

[0035]Prepare large unilamellar liposomes according to the extrusion method published by Hope et al. Take an appropriate amount of phospholipid mixture (lecithin / cholesterol, 55:45 molar ratio) and isotope-labeled cholesteryl ester [3H]-cholesteryl hexadecyl ether (radioactive (radioactivity is 1.33Ci / 4mol)) was co-dissolved in chloroform / methanol, added nitrogen gas in the test tube to dry to form a uniform mixed phospholipid film, and dried overnight in vacuum. Add HBS buffer (20 mmol / L Hepes, 150 mmol / L NaCl, pH 7.5) or HBS buffer containing calcein (100 mM) into the test tube containing the phospholipid membrane, shake rapidly to hydrate and dissolve the phospholipid membrane. The obtained multilamellar vesicles (MLVs) were quick-frozen in liquid nitrogen and then hydrolyzed in 55°C water for 5 rounds. Lamellar liposomes were repeatedly extruded through a double-layer 100 nm pore membrane (Nucleopore) for 10 times to obtain the required l...

Embodiment 2

[0056] 2.1 Drugs and reagents

[0057] Fluorescently labeled phospholipids N-(7-nitro2,1,3-benzoxadiazol-4-yl) phosphatidy lethanolamine (NBD-PE), rhodamine phosphatidy lethanolamine (Rh-PE ), distearoylphosphatidylcholine (DSPC), dipalmitoyl phosphatidylcholine (DPPC) and dimiristoyl phosphatidylg Lycerol (DMPG) was purchased from (Avanti polar lipids company in the United States), lecithin (EPC, 98% purity) and cholesterol (Chol, 98% purity) were provided by Xi’an Libang Pharmaceutical Co., Ltd., and MH medium was a product of Gibco Company in the United States. Poly(2-ethylacrylic acid) (molecular weight: 20,000) was provided by Fu Jingguo, a researcher at the Institute of Chemistry, Henan Academy of Sciences, and the rest of the reagents were of domestic analytical grade.

[0058] 2.2 Drugs and reagents

[0059] Extruder (Canada Lipex Biomembranes Company), LS-50B Fluorescence Spectrophotometer (U.S. Perkin-Elmer Company), Nicomp 370 Laser Scattering Particle Size Analyze...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides an intelligent macromolecular amphoteric lipid material as well as a synthesis method and an application thereof in a complex drug delivery preparation, belongs to the technicalfield of intelligent liposomes, and aims to solve the problems that different microenvironment changes, such as vascular permeability increase, local acidity increase and local temperature rise and the like, are generated at disease parts of a human body. The novel material disclosed by the invention can change the characteristics of macromolecular configuration along with the changes of the microenvironment of a human body, and the purpose of rapidly releasing drugs at lesion parts is achieved by utilizing the influence of the deformed macromolecular characteristics on the interference capability of a cell phospholipid bilayer membrane, so that the effects of intelligently administering drugs and improving the treatment diseases are achieved.

Description

technical field [0001] The invention relates to the technical field of intelligent liposomes, in particular to a synthesis method of an intelligent polymer amphoteric lipid material and its application in complex drug delivery preparations. Background technique [0002] A large number of animal and clinical experimental data have demonstrated that liposome-encapsulated anticancer drugs, antibiotics and antifungal compounds can improve drug efficacy. The advantage of liposome preparations is that they can change drug metabolism in vivo and organ distribution of drugs, which can significantly reduce the side effects of drugs [Deng YJ, 2007]. In particular, it should be pointed out that liposomes can be selectively enriched in new tumors, infection and inflammation sites, so drugs can be targeted to the lesion together with liposomes [Chonn and Cullis, 1995]. [0003] Although we have successfully encapsulated various drugs in liposomes and sent them to lesions and diseased ce...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K9/127A61K47/32A61K47/24A61K47/28
CPCA61K9/1271A61K47/24A61K47/28A61K47/32
Inventor 郑岩
Owner XIAN LIBANG PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap