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Fermentation medium for producing demethylated aureomycin

A technology of demethylaureomycin and fermentation medium, which is applied in the direction of microorganism-based methods, fermentation, and growth stimulation by adding compounds, can solve the problems of high price, difficult application of medium, and increase of fermentation operation cost, etc., and achieves The effect of increasing content

Active Publication Date: 2020-08-21
HEBEI SHENGXUE DACHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

From an economic point of view, the price of D-methionine is more expensive than that of bulk raw materials, which will greatly increase the operating cost of fermentation
Chinese patent CN201310575478.2 discloses a medium and a culture method for producing demethylaureomycin. The demethylaureomycin fermentation cycle is 180h, and the fermentation titer is 13000mg / L, but its demethylaureomycin fermentation process It is a three-stage fermentation, and the medium contains fish oil and other animal-derived raw materials. According to the requirements of veterinary drug GMP, the medium is difficult to be applied in industry

Method used

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  • Fermentation medium for producing demethylated aureomycin
  • Fermentation medium for producing demethylated aureomycin
  • Fermentation medium for producing demethylated aureomycin

Examples

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example 1

[0015] a Preparation of fermentation medium: Weigh 28g of cornstarch, 0.2g of molasses, 14g of high-temperature soybean powder, 10g of corn oil, 6g of corn steep liquor, 2.2g of calcium carbonate, 0.8g of calcium chloride, 0.48g of ammonium sulfate, and 0.06 potassium dihydrogen phosphate g, L-lysine salt 2g, a-amylase 0.002g. First gelatinize the starch, add amylase, cool for later use, then dissolve the other ingredients in purified water, and finally combine the two parts of the liquid, set the volume to 400ml, adjust the pH to 5.5, and evenly distribute it into ten 250ml shaker flasks sterilized at 121°C for 30 minutes, and cooled to room temperature for use.

[0016] b Take 2ml of mature seed culture solution and insert it into ten 250ml shake flasks equipped with 40ml of fermentation medium, culture temperature is 28 degrees, shaker speed is 200rpm, culture period is 168 hours. After the fermentation is terminated, use high pressure liquid chromatography to measure the ...

example 2

[0019] a Preparation of fermentation medium: Weigh 28g of cornstarch, 0.4g of molasses, 14g of high-temperature soybean powder, 10g of corn oil, 6g of corn steep liquor, 2.2g of calcium carbonate, 1.2g of calcium chloride, 0.48g of ammonium sulfate, and 0.06 potassium dihydrogen phosphate g, L-lysine salt 2g, a-amylase 0.002g. First gelatinize the starch, add amylase, cool for later use, then dissolve the other ingredients in purified water, and finally combine the two parts of the liquid, set the volume to 400ml, adjust the pH to 5.5, and evenly distribute it into ten 250ml shaker flasks sterilized at 121°C for 30 minutes, and cooled to room temperature for use.

[0020] b Take 2ml of mature seed culture solution and insert it into ten 250ml shake flasks equipped with 40ml of fermentation medium, culture temperature is 28 degrees, shaker speed is 200rpm, culture period is 168 hours. After the fermentation is terminated, use high pressure liquid chromatography to measure the ...

example 3

[0024] a Preparation of fermentation medium: Weigh 28g of corn starch, 0.6g of molasses, 14g of high-temperature soybean powder, 10g of corn oil, 6g of corn steep liquor, 2.2g of calcium carbonate, 1.6g of calcium chloride, 0.48g of ammonium sulfate, and 0.06 potassium dihydrogen phosphate g, L-lysine salt 2g, a-amylase 0.002g. First gelatinize the starch, add amylase, cool for later use, then dissolve the other ingredients in purified water, and finally combine the two parts of the liquid, set the volume to 400ml, adjust the pH to 5.5, and evenly distribute it into ten 250ml shaker flasks sterilized at 121°C for 30 minutes, and cooled to room temperature for use.

[0025] b Take 2ml of mature seed culture solution and insert it into ten 250ml shake flasks equipped with 40ml of fermentation medium, culture temperature is 28 degrees, shaker speed is 200rpm, culture period is 168 hours. After the fermentation is terminated, use high pressure liquid chromatography to measure the...

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Abstract

The invention belongs to the technical field of fermentation culture, and particularly relates to a fermentation medium for producing demethylated aureomycin, the fermentation medium contains a carbonsource, a nitrogen source and a phosphorus source, molasses is also added as a source for providing biotin and pantothenic acid, and calcium chloride is also added to replace sodium chloride as an inorganic salt source. According to the method, the titer of demethylated aureomycin can be increased, the content of impurity demethylated tetracycline can be reduced, and the method is efficient, simple and low in cost.

Description

technical field [0001] The invention belongs to the technical field of fermentation culture, and in particular relates to a fermentation medium for producing desmethylchlortetracycline. Background technique [0002] Demethylchlortetracycline is a tetracyclic antibiotic, the English name is demethylchlortetracycline, which is characterized by stronger action than tetracycline and oxytetracycline, and more stable than aureomycin. As a member of the tetracyclic antibiotics, the antibacterial mechanism of demethylchlortetracycline is that it can specifically bind to the A position of the 30S subunit of the bacterial ribosome, thereby preventing the connection of the aminoacyl-tRNA to this position, thereby making the bacterial protein Delayed or impossible to craft. It is clinically used for pneumonia, urinary tract infection, gonorrhea, bacillary dysentery and scarlet fever in children. In addition to its clinical use, demethylchlortetracycline is also a precursor for the syn...

Claims

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Application Information

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IPC IPC(8): C12P29/00C12N1/20C12N1/38C12R1/485
CPCC12P29/00C12N1/20C12N1/38
Inventor 韩冰孙翠玲刘聪洁王绘砖徐珍袁昉
Owner HEBEI SHENGXUE DACHENG PHARMA
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