Production method of iron-fortified whey protein milk

A technology of whey protein and milk, applied in dairy products, milk preparations, protein food processing, etc., can solve the problems of rancidity, bad taste, etc., achieve the effect of large iron supplementation, reduce gastric damage, and good stability

Pending Publication Date: 2020-09-11
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Iron ions in emulsified lipids catalyze the generation of free radicals leading to rancidity and unpleasant taste

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. The preparation method of whey protein iron nanoparticles: take whey protein isolate (WPI) as raw material, prepare the whey protein solution that mass concentration is 40mg / mL with distilled water, in the whey protein solution with 1:5 Mass ratio of FeSO added 4 , using HCl and NaOH to adjust the pH value to 6, magnetic stirring at 25 °C for 40 min for sufficient chelation reaction, centrifugation at 3800 r / min for 10 min to remove insoluble protein and iron, and retain the supernatant. Then the supernatant was centrifuged through a 10kDa ultrafiltration centrifuge tube at 5000r / min for 5min for ultrafiltration to remove free iron ions, and the retained part was divided into frozen trays with a thickness of 5mm, and placed in a pre-freezing temperature of - In the freezer at 80°C, after a pre-freezing time of 8 hours, it was transferred to a vacuum freeze dryer, the temperature was set at -40°C, and the freeze-drying time was 12 hours to obtain whey protein iron nan...

Embodiment 2

[0022] 1. The preparation method of whey protein iron nanoparticles: take whey protein isolate (WPI) as raw material, prepare the whey protein solution that mass concentration is 40mg / mL with distilled water, add 1:10 to the whey protein solution Mass ratio of FeSO added 4 , using HCl and NaOH to adjust the pH value to 7, magnetic stirring at 25 ° C for 60 min for sufficient chelation reaction, centrifugation at 3800 r / min for 10 min to remove insoluble protein and iron, and retain the supernatant. Then the supernatant was passed through a 10kDa ultrafiltration centrifuge tube at 5000r / min for 5min to carry out ultrafiltration to remove free iron ions, and the retained part was divided into freezing plates with a thickness of 10mm, and placed in a pre-freezing temperature of - In a freezer at 80°C, after a pre-freezing time of 10 hours, it was transferred to a vacuum freeze dryer, the temperature was set at -30°C, and the freeze-drying time was 12 hours to obtain whey protein ...

Embodiment 3

[0025] 1. The preparation method of whey protein iron nanoparticles: take whey protein isolate (WPI) as raw material, prepare the whey protein solution that mass concentration is 40mg / mL with distilled water, add 1:15 to the whey protein solution Mass ratio of FeSO added 4 , using HCl and NaOH to adjust the pH value to 6, magnetic stirring at 25 °C for 40 min for sufficient chelation reaction, centrifugation at 3800 r / min for 10 min to remove insoluble protein and iron, and retain the supernatant. Then the supernatant was centrifuged at 4000r / min for 5min through a 10kDa ultrafiltration centrifuge tube for ultrafiltration to remove free iron ions, and the retained part was divided into frozen trays with a thickness of 10mm, and placed in a pre-freezing temperature of - In the freezer at 70°C, after 12 hours of pre-freezing time, move to a vacuum freeze dryer, set the temperature at -30°C, and freeze-dry for 18 hours to obtain whey protein iron nanoparticles.

[0026] 2. Prepa...

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PUM

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Abstract

The invention relates to a production method of iron-fortified whey protein milk, and belongs to the technical field of health-care food. With whey protein isolate as a raw material, the whey proteinisolate and ferrous ions are chelated, and through operation of magnetic stirring, centrifugation, ultrafiltration, vacuum freeze drying and the like, whey protein and iron nanoparticles which are small in particle size, good in stability and easy to absorb are produced, and are added into milk to produce the iron-fortified whey protein milk. According to the production method of the iron-fortified whey protein milk, the whey protein isolate and the ferrous ions are used for producing the whey protein and iron nanoparticles so as to produce the iron-fortified whey protein milk, oxidation of fat in the milk can be prevented, organic combination of food and a medicine is achieved, the iron-fortified whey protein milk has a good iron supplement effect, absorption in the intestinal tract of ahuman body can be met, and gastric injuries are reduced. The process route is simple and direct, the equipment investment is little, and the feasibility of industrial popularization is good.

Description

technical field [0001] The invention belongs to the technical field of health food, and in particular relates to a formula for fortified milk with whey protein iron and a preparation method thereof. Whey protein isolate is used as a raw material to chelate with ferrous ions, and whey protein iron nanoparticles are prepared and added to milk A whey protein-iron-fortified milk was prepared in . Background technique [0002] Iron is an essential trace element for the human body, participates in the synthesis of hemoglobin and various enzymes, and plays an important role in various physiological processes such as oxygen transport, immune regulation, nucleic acid synthesis, and gene expression regulation. Iron deficiency can cause iron deficiency anemia, weakness, fatigue, glossitis, keratitis, hair loss, and itching, and may also cause cognitive impairment, reduced physical mobility, and miscarriage in children. Pregnant women and young children are the main groups of iron defi...

Claims

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Application Information

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IPC IPC(8): A23C9/152A23J3/00A23J3/08
CPCA23C9/1522A23C9/1526A23J3/08A23J3/00
Inventor 奚春宇刘于嘉康乃馨孙正伟
Owner JILIN UNIV
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