A multifunctional injectable hydrogel for photothermal therapy of tumors and bone tissue repair and its preparation method

A photothermal therapy, bone tissue technology, applied in tissue regeneration, pharmaceutical formulations, prostheses, etc., can solve the problems of insufficient photothermal therapy effect, affecting the long-term curative effect of tumors, low photothermal agent enrichment rate, etc. Good photothermotherapy effect, good new osteoinductivity, good effect of thermosensitive properties

Active Publication Date: 2021-10-15
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, photothermal therapy for the treatment of bone tumors is still in further research, and some significant problems need to be resolved urgently
For example, as an important photothermal conversion material for photothermal therapy of tumors, nano-photothermal agents have a significant impact on the effect of photothermal therapy, and the enrichment rate of photothermal agents is low when delivered to tumor sites through intravenous injection. to photothermal therapeutic effect
Although in situ injection of nano photothermal agent can enhance the local photothermal treatment temperature, due to human metabolism and blood circulation, the residence time of photothermal agent in the tumor site is short, which greatly affects the long-term curative effect of the tumor.

Method used

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  • A multifunctional injectable hydrogel for photothermal therapy of tumors and bone tissue repair and its preparation method
  • A multifunctional injectable hydrogel for photothermal therapy of tumors and bone tissue repair and its preparation method
  • A multifunctional injectable hydrogel for photothermal therapy of tumors and bone tissue repair and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039]1) Dissolve hydrated glucose into deionized water, and configure it into a 0.5mol / L glucose solution after fully dissolving;

[0040] 2) Put 200mL of glucose solution in a 300mL autoclave, react at 170°C for 6h, wash the obtained reaction products with deionized water and absolute ethanol three times, collect them by centrifugation, and place them at 50°C Vacuum drying for 24 hours to obtain micro-nano carbon spheres;

[0041] 3) dissolving chitosan in 1% acetic acid solution, fully stirring, standing for 12 hours, removing undissolved impurities and air bubbles, and obtaining a chitosan solution with a concentration of 3%;

[0042] 4) adding the micro-nano carbon spheres obtained above into the chitosan solution, wherein 1.25 mg of carbon spheres were added to each milliliter of the chitosan solution, and after ultrasonic vibration and magnetic stirring, the solution was uniformly mixed to obtain solution A;

[0043] 5) Dissolve β-sodium glycerophosphate and sodium hya...

Embodiment 2

[0046] 1) Dissolve sucrose into deionized water, and after fully dissolving, configure it into a 1mol / L sucrose solution;

[0047] 2) Put 200mL of sucrose solution in a 300mL autoclave, react at 180°C for 8h, wash the obtained reaction products with deionized water and absolute ethanol three times, collect them by centrifugation, and place them at 50°C Vacuum drying for 24 hours to obtain micro-nano carbon spheres;

[0048] 3) dissolving chitosan in 1.5% acetic acid solution, fully stirring, standing for 6 hours, removing undissolved impurities and air bubbles, and obtaining a chitosan solution with a concentration of 5%;

[0049] 4) adding the micro-nano carbon spheres obtained above into the chitosan solution, wherein 0.2 mg of carbon spheres are added to each milliliter of the chitosan solution, and after ultrasonic vibration and magnetic stirring, the solution is uniformly mixed to obtain solution A;

[0050] 5) Dissolve β-sodium glycerophosphate and sodium hyaluronate in...

Embodiment 3

[0053] 1) Dissolve fructose in deionized water, and after fully dissolving, prepare a 2mol / L fructose solution;

[0054] 2) Put 200mL of sucrose solution in a 300mL autoclave, react at 190°C for 10h, wash the obtained reaction products with deionized water and absolute ethanol three times, collect them by centrifugation, and place them at 80°C Vacuum drying for 24 hours to obtain micro-nano carbon spheres;

[0055] 3) dissolving chitosan in 2% acetic acid solution, fully stirring, standing for 12 hours, removing undissolved impurities and air bubbles, to obtain a 1% chitosan solution;

[0056] 4) adding the micro-nano carbon spheres obtained above into the chitosan solution, wherein 8 mg of carbon spheres were added to each milliliter of the chitosan solution, and subjected to ultrasonic vibration and magnetic stirring to make the solution evenly mixed to obtain solution A;

[0057] 5) Dissolve β-sodium glycerophosphate and sodium hyaluronate in deionized water at the same ti...

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Abstract

The invention discloses a multifunctional injectable hydrogel for tumor photothermal therapy and bone tissue repair and a preparation method thereof, belonging to the field of biomedical materials. The steps are as follows: firstly, micro-nano carbon spheres are synthesized by a hydrothermal carbonization method. Next, add the micro-nano carbon spheres into the chitosan solution and stir evenly to obtain solution A. Again, dissolve sodium β-glycerophosphate and sodium hyaluronate into deionized water at the same time, and stir thoroughly to obtain solution B. Finally, in an ice-bath environment, solution B was added to solution A, stirred evenly, and sterilized by ultraviolet light for 24 hours to obtain an injectable composite hydrogel. The invention has good biocompatibility; it is liquid at room temperature, can be directly injected into bone tumor lesions, and can quickly form a gel in situ at a body temperature of 37°C, realizing effective fixation of photothermal agents and bone defects The rapid filling of bone tumor cells can be achieved through local photothermal therapy, and at the same time, it can effectively promote the repair of bone tissue and realize the comprehensive treatment of bone tumors.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and more specifically relates to a multifunctional injectable hydrogel for photothermal treatment of tumors and bone tissue repair and a preparation method thereof. Background technique [0002] Bone tumors are tumors that develop in the bone or the tissues surrounding it, causing abnormal growth of tissue in the body. Malignant bone tumors have a high incidence and are often difficult to cure. It is worth noting that most malignant tumors such as lung cancer, prostate cancer, breast cancer, and kidney cancer are prone to cancer metastasis during their development, and bone tissue is the main metastatic site of the above tumors, resulting in the formation of metastatic bone tumors. Bone tumors are usually accompanied by serious complications such as bone pain, osteolysis, fracture, and bone deformity. These complications bring great suffering to patients, reduce the quality of life, and caus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/20A61L27/50A61L27/52A61L27/54C08J3/075C08L5/04C08L5/08C08K5/52C08K7/18
CPCA61L27/20A61L27/50A61L27/52A61L27/54A61L2300/108A61L2300/416A61L2400/06A61L2400/12A61L2430/02C08J3/075C08J2305/04C08J2305/08C08J2405/04C08J2405/08C08K5/52C08K7/18C08L5/08
Inventor 吴承伟魏成雄金鑫张伟马建立
Owner DALIAN UNIV OF TECH
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