Magnetic renewable antibacterial agent and preparation method thereof
An antibacterial agent and magnetic technology, applied in the field of magnetic regenerable antibacterial agent and its preparation, can solve the problems of antibacterial agent loss, difficulty, easy loss, etc., and achieve the effect of solving difficult processing
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Embodiment 1
[0029] Preparation of graphene oxide.
[0030] The 250mL check bottle was put into a freezing tank whose system temperature was 0°C, and 27.6mL of concentrated sulfuric acid (98wt%H 2 SO 4 ), keep the temperature below 5°C, slowly add 1.2g graphite powder while stirring, continue stirring for 1.5h, then slowly add 3.6g KMnO 4 After stirring for 10 minutes, the solution was purple-green. The reaction temperature was controlled below 20°C and the reaction was continued for 1 hour. Then, the reaction flask was placed in an oil bath at 35°C for 3 hours, and 55 mL of deionized water was added slowly, and the temperature was raised to 95°C for continuous stirring. 15min, then add 140mL of water to stop the reaction. 6mL hydrogen peroxide (30wt%H 2 o 2 ) into the reaction system, observe the color change of the solution, stop dripping hydrogen peroxide when the solution just turns yellow, pour off the supernatant, rinse twice with 2wt% HCl solution, and then wash three times with...
Embodiment 2
[0032] Fe 3 o 4 Preparation of surface reversible addition-fragmentation chain transfer agents.
[0033] Fe 3 o 4 Nanoparticles are prepared by the method of Ding, Y.L., etc. Mater.Sci.Eng.C.48,487-498 (2015), and then prepared on Fe by the method of Makhluf, S.B., etc. Small.4,1453-1458 (2008). 3 o 4 Amino groups were introduced into the surface of nanoparticles to obtain aminated Fe 3 o 4 Nanoparticles (amino group content 16.06 mmol / g). Add 4-cyano-4-(thiobenzoyl)valeric acid (0.1118g, 0.4mmol) and 1-hydroxybenzotriazole (0.0405g, 0.3mmol) into a 150mL branched-necked flask, pump nitrogen After three times, N 2 Under protection, 30 mL of dichloromethane was added with a disposable syringe, and the aminated Fe dissolved in 30 mL of dichloromethane 3 o 4 Nanoparticles (0.0125g, 0.2mmol) were added to the reaction flask, and after stirring at room temperature for 30min, 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride dissolved in 10mL of dichloromethane (0...
Embodiment 3
[0035] Fe 3 o 4 Preparation of grafted poly-4-vinylbenzaldehyde hybrids.
[0036] The synthetic Fe of above-mentioned embodiment 2 3 o 4 Surface reversible addition-fragmentation chain transfer agent (0.09g, 0.1mmol) and 4-vinylbenzaldehyde (3.83mL, 30mmol) were dissolved in 30mL dioxane, and azobisisobutyronitrile (0.0821g, 0.5 mmol) as an initiator, reacted in an oil bath at 70°C under the protection of nitrogen for 12 hours, put it in an ice-water bath to terminate the reaction, precipitated and washed the reaction product with diethyl ether, and after continuous precipitation and washing for 3 times, freeze-dried the purified product and dried it under vacuum at 35°C Dry to constant weight to obtain solid powdery Fe 3 o 4 The grafted poly-4-vinylbenzaldehyde hybrid was 3.09 g, and the yield was 76.2%.
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