Application of nitrile hydratase in catalyzing hydration reaction of cyanopyrazine compound to generate amide pyrazine compound

A technology for catalyzing cyanopyrazine and nitrile hydratase, which is applied in the direction of enzymes, lyases, and carbon-oxygen lyases, can solve the problems of complex synthesis, low conversion rate and total yield, cumbersome process, etc., and achieve high conversion rate , no side effects, the effect of green environmental protection operation
CN111662938AActive Publication Date: 2020-09-15ZHEJIANG SANMEN HYGECON PHARMA CO LTD
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG SANMEN HYGECON PHARMA CO LTD
Publication Date
2020-09-15

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Abstract

The invention provides an application of nitrile hydratase in catalyzing a hydration reaction of a cyanopyrazine compound to generate an amide pyrazine compound, and belongs to the technical field ofbiochemical engineering. The nitrile hydratase is derived from Agrobacterium tumefaciens, Stappia aggregata IAM 12614 or Sinorhizobium meliloti. The cyanopyrazine compound is used as a substrate, in-vitro nitrile hydratase or a cell expressing the nitrile hydratase is used as a catalyst, the hydration reaction is carried out, and the amide pyrazine compound can be obtained. The applied raw material is high in conversion rate and free of side reaction, and the product is easy to separate and purify and is relatively high in purity; and compared with a traditional chemical catalysis process, theprocess is environmentally friendly and easy and convenient to operate, and the yield exceeds 99%.
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Description

technical field

[0001] The invention relates to the field of biochemical technology, in particular to the application of nitrile hydratase in catalyzing the hydration reaction of cyanopyrazine compounds to generate amide pyrazine compounds. Background technique

[0002] Amidepyrazine (or pyrazinamide) is an important anti-tuberculosis drug, which has a good effect on tuberculosis. In recent years, it has been found that amidopyrazine has a good bactericidal effect on recalcitrant bacteria, so it is widely used in clinic; and amidopyrazine can be used as an intermediate for the preparation of various antibacterial and antiviral drugs. For example, derivatives of amide pyrazine: 6-fluoro-3-hydroxy-2-amide pyrazine, also known as Favipiravir (English: Favipiravir, also known as Avigan or favilavir), is an antiviral drug, produced by Professor Koyasu Shiraki of the Faculty of Medicine of Toyama University in Japan and Toyama Chemical Industry (now Fujifilm Toyama Chemical), a s...

Claims

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