Amphiphilic polymer-mediated controllable assembly of gold nanoparticles and its preparation and application
An amphiphilic polymer, gold nanotechnology, applied in nanomedicine, nanotechnology, nanotechnology and other directions, can solve the problem of unknown delivery relationship and other problems, and achieve the effect of wide excitation wavelength range, low toxicity and good stability
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Embodiment 1
[0064] The amphiphilic polymer-mediated gold nanoassembly material is prepared by the following method:
[0065] At room temperature, add 18.75μL hexanethiol (8M) (solvent is water), 0.2g F127 and 10mL water into a 25mL three-necked flask, stir well (stirring 0.5h) to form micelles, and the hydrophobic The ligand and the hydrophobic block of F127 self-assemble through hydrophobic interaction; then add 1mL tetrachloroauric acid (50mM, solvent is water), stir vigorously at room temperature (stirring speed is 1000rpm) until colorless and form gold monovalent thiol After the complex, first adjust the pH of the solution to 11 with 0.1M sodium hydroxide, add a reducing agent to react (add 6.8 μL tetrakishydroxymethyl phosphorus chloride (50 mM) and stir the reaction with magnetic force at room temperature for 8 hours to form a luminescence position at 520 nm Amphiphilic polymer-mediated gold nanoassemblies (AuNAs-520); or add 10 μL dimethylamine borane (25mM) under magnetic stirring...
Embodiment 2
[0083] Take 100 μL of the synthesized amphiphilic polymer-mediated gold nanoassembly (solvent is water), add 100 μL of FBS 10% (v / v) and dilute to 1 mL, the control group directly dilutes the material (using diluted with water) to 1 mL, incubated in an incubator at 37°C for 30 minutes, and then measured their hydrated particle size with a particle size analyzer (Malvern Mastersizer). To identify their FBS adsorption capacity.
[0084] Figure 11 It is the hydration particle size diagram of the amphiphilic polymer-mediated gold nanoassembly and bovine serum albumin incubation in Example 2. Figure 11 It is the hydrated particle size of the amphiphilic polymer-mediated gold nanoassembly itself, and the hydrated particle size after incubation with FBS at 37°C for 30 minutes. In the figure, the hydrated particle size of AuNAs-520 is after incubation with FBS Increased from 30.8±5.5nm to 50.0±10.9nm, the hydrated particle size of AuNAs-610 increased from 29.6±3.4nm to 30.8±6.0nm ...
Embodiment 3
[0086] The present invention prepares amphiphilic polymer-mediated gold nano-assemblies (prepared in Example 1) for detecting the toxicity and uptake ability of MDA-MB-231 breast cancer cells: 10 μL of different concentrations prepared in Example 1 The nanomaterials were mixed with 90 μL DMEM medium, and the cells were cultured for 24 hours, and the toxicity of the amphiphilic polymer-mediated gold nanoassembly was studied at different concentrations through the MTT detection kit.
[0087] Mix 10 μL of the nanomaterial (5 mg / L) prepared in Example 1 with 490 μL of DMEM medium, and culture the cells for 1, 3, 6, 12, and 24 hours respectively, then suck out the culture medium, wash with DPBS three times, and add freshly prepared Aqua regia dissolves the gold nano-assemblies in the cells, and then uses inductively coupled plasma to measure the gold element content.
[0088] Figure 12 It is the analysis results of cytotoxicity experiments of amphiphilic polymer-mediated gold nan...
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