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Application of fucoid oligosaccharide in the preparation of intestinal prebiotics

A technology of fucoidal oligosaccharides and prebiotics, applied in the biological field, can solve problems such as fucoidal oligosaccharides function research, achieve the effect of reducing membrane transport function, promoting the production of short-chain fatty acids, and improving the level of intestinal microbial flora

Active Publication Date: 2021-11-02
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the function of fucooligosaccharides hydrolyzed by capsular exopolysaccharide produced by Kosakonia sp. has not been studied

Method used

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  • Application of fucoid oligosaccharide in the preparation of intestinal prebiotics
  • Application of fucoid oligosaccharide in the preparation of intestinal prebiotics
  • Application of fucoid oligosaccharide in the preparation of intestinal prebiotics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, the preparation of microbial exopolysaccharide (EPS)

[0031] EPS is fermented and produced by Kosakonia sp.CCTCC M2018092 strain under fed-batch conditions. The specific steps are as follows: take Kosakonia sp.CCTCC M2018092 strain and culture it in a 250mL shake flask containing 30mL medium at 30°C and 200rpm for 20 hours , and then 30ml of bacterial culture solution was transferred to a 15L size fermenter at 30°C and 300rpm for pre-growth culture for 13 hours (air flow 1.5m 3 / h). Afterwards, get the bacterial liquid of 3L pre-growth and transfer in the 50L fermenter (containing 30L medium) and carry out fed-batch fermentation. Use peristaltic pump according to residual sugar amount to begin to add the glucose solution of 200g / L in batches with the speed of 0.9-3.8rpm after fermentation begins 13h. The ventilation volume of the fermenter is 1.5m 3 / h, the dissolved oxygen concentration is controlled above 10% by automatically adjusting the speed (300...

Embodiment 2

[0037] Embodiment 2, preparation of low molecular weight fucoidal oligosaccharides

[0038] The hydrolyzed polysaccharide prepared in Example 1 was dissolved in deionized water to prepare a polysaccharide aqueous solution with a mass fraction of 5%, and trifluoroacetic acid was added to make the final concentration 0.1M, and hydrolyzed in an oil bath at 100°C for 0.5h, 1h, 1.5h, After 2h and 3h, the obtained polysaccharide hydrolyzate was removed by using a 200Da nanofiltration membrane to remove trifluoroacetic acid, concentrated to a small volume by rotary evaporation at 40°C, and freeze-dried to obtain an oligosaccharide sample. The molecular weight was determined by high performance liquid chromatography. The chromatographic column was G3000PWXL. The column temperature is 40°C, and the mobile phase is 0.1M NaNO 3, the injection volume was 15 μl, and the results are shown in Table 1. The results show that the extraction rate of fucoidan oligosaccharides from trifluoroaceti...

Embodiment 3

[0044] Example 3, test tube simulated intestinal fermentation of fucoidal oligosaccharides

[0045] Take 1L of fermentation medium, the medium components are as follows: peptone 2g / L; yeast extract 2g / L; NaCl 0.1g / L; K 2 HPO 4 0.04g / L; KH 2 PO 4 0.04g / L; MgSO 4 ·7H 2 O 0.01g / L; CaCl 2 ·6H 2 O 0.01g / L; NaHCO 3 2g / L; Tween 80 2mL / L, add heme at 0.02g / L after sterilization; 10mL / L vitamin K 1 ; 0.5g / L bile salt; 0.5g / L cysteine ​​hydrochloride, adding fucoidal oligosaccharides by mass fraction 0.1%.

[0046] First put 6 test tubes into 25ml culture medium, inoculate with 10% fecal bacteria liquid, and pass through anaerobic gas for 5min (85%N 2 , 10% CO 2 , 5%H 2 ), and seal with paraffin liquid, take a sample every 12h, measure the pH and gas of the fermentation broth, the results are as follows image 3 shown. The results showed that the pH drop rate of the fermentation broth added with 0.1% fucoidal oligosaccharide was higher than that of the control group.

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Abstract

The invention discloses the application of fucoidal oligosaccharides in the preparation of intestinal prebiotics. The use of fucoidal oligosaccharides has the functions of promoting carbohydrate metabolism, sugar biosynthesis and metabolism, reducing membrane transport function, enhancing digestive system function or reducing disease infection. function, promote the production of short-chain fatty acids in the intestine, improve the level of intestinal microbial flora, and be used to prepare intestinal prebiotics for human or livestock and poultry animals, and have broad application prospects.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of fucoidal oligosaccharides in the preparation of intestinal prebiotics. Background technique [0002] Fucose is a rare L-configuration of 6-deoxyhexose, commonly found in microbial exopolysaccharides, brown algae and mammals. Fucose modification has been identified to be involved in many biological functions, including immune regulation and cancer. Sulfate-containing fucoidans, which are abundant in brown algae, have anticoagulant, antithrombotic, immunomodulatory, anticancer, and antiproliferative activities. However, the yield of fucoidan in plants or algae is low, and its composition varies with climate and seasons. The production of fucose-rich exopolysaccharides by microorganisms is considered to be a better alternative, given the advantages of higher growth rates and easier control of production conditions. In recent decades, the highest yield of fucose-con...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/702A61P1/00A61P1/14A23L33/125
CPCA23V2002/00A61K31/702A23L33/125A61P1/00A61P1/14A23V2200/32
Inventor 邹祥王振宇马巍李姗姗徐兴然
Owner SOUTHWEST UNIV
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