Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Pearl shell whitening factor, and preparation method and application thereof

A technology of factor and pearl, applied in the field of freeze-dried powder of pearl oyster whitening factor and its preparation

Pending Publication Date: 2020-10-30
OSMUN BIOLOGICAL
View PDF8 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The problem to be solved in this application is how to produce a product with endothelin inhibition through a large number of low-cost by-products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pearl shell whitening factor, and preparation method and application thereof
  • Pearl shell whitening factor, and preparation method and application thereof
  • Pearl shell whitening factor, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A preparation method of pearl oyster whitening factor, the method comprising the steps of:

[0032] Step 1) Clean the clam shells, remove epidermal dirt and algae, dry and pulverize them, and pass through a 200-mesh sieve to obtain small particle clam shell powder;

[0033] Step 2) Mix clam shell powder and 15% acetic acid solution in a mass ratio of 1:20, stir at 60°C and 200 rpm for 3 hours, and separate the solid to obtain crude protein;

[0034] Step 3) After cleaning the crude protein with deionized water (the TDS value in the cleaning solution is 150ppm), mix it with the buffer solution PBS with a pH value of 7.5 at a mass ratio of 1:5, and process the mixed solution through an ultra-high pressure of 100Mpa at 40°C for 30min Then, mix and react with trypsin under ultrasound at 60°C for 3 hours, and the mass volume ratio of trypsin to the mixture is 1:100g / mL;

[0035] Step 4) After stirring at 85°C for 30 minutes to inactivate the enzyme, press filter through a 0...

Embodiment 2

[0038] A preparation method of pearl oyster whitening factor, the method comprising the steps of:

[0039] Step 1) Clean the clam shells, remove epidermal dirt and algae, dry and pulverize them, and pass through a 200-mesh sieve to obtain small particle clam shell powder;

[0040] Step 2) Mix clam shell powder and 10% lactic acid solution in a mass ratio of 1:20, stir at 60°C and 200 rpm for 4 hours, and separate the solid to obtain crude protein;

[0041] Step 3) After cleaning the crude protein with deionized water (the TDS value in the cleaning solution is 100ppm), mix it with the pH value 7.5 buffer solution Tris-HCl at a mass ratio of 1:10, and pass the mixed solution through ultra-high pressure 100Mpa, 40°C After treatment for 30 minutes, mix and react with neutral protease at 60°C for 3 hours under ultrasound, and the mass volume ratio of neutral protease to the mixed solution is 1:100g / mL;

[0042]Step 4) Obtain the supernatant after stirring at 85°C for 35min to inac...

Embodiment 3

[0045] A preparation method of pearl oyster whitening factor, the method comprising the steps of:

[0046] Step 1) Clean the clam shells, remove epidermal dirt and algae, dry and pulverize them, and pass through a 200-mesh sieve to obtain small particle clam shell powder;

[0047] Step 2) Mix clam shell powder and citric acid solution with a mass fraction of 20% according to a mass ratio of 1:5, stir at 50°C at a speed of 100 rpm for 3 hours to fully decompose, and separate the solid to obtain crude protein;

[0048] Step 3) Wash the crude protein with deionized water until the TDS value in the cleaning solution is 120ppm, then mix it with a citric acid buffer solution with a pH value of 7.0 at a mass ratio of 1:15, and process the mixed solution through ultra-high pressure 200Mpa and 45°C After 40 minutes, mix and react with papain under ultrasound at 40°C for 7 hours. The mass-volume ratio of papain to the mixture is 1:100g / mL.

[0049] Step 4) Obtain the supernatant after ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method for a pearl shell whitening factor. The preparation method comprises the following steps: 1) crushing clamshells, and conducting screening to obtain small-particle clamshell powder; 2) mixing the clamshell powder with an aqueous weak acid solution, conducting stirring and decomposing, and separating out a solid, namely crude protein; 3) cleaning the crude protein with deionized water, mixing the cleaned crude protein with a buffer solution having a pH value of 6.0-7.5, treating the mixed solution for 20-40 minutes at an ultrahigh pressure of 100-200Mpa and a temperature of 40-80 DEG C, and subjecting the mixed solution and protease to mixing and reacting for 2-7 hours at a temperature of 40-60 DEG C under ultrasonic conditions; 4) carrying outstirring for 20-50 minutes at 85-100 DEG C for enzyme deactivation, and performing filter pressing through a filter membrane to obtain a sterile filtrate; 5) mixing a freeze-drying protective agent with the filtrate, conducting pre-freezing, and then performing freeze-drying in a vacuum freeze-drying bin for 12-20 hours so as to finally obtain freeze-dried powder with a moisture content of 2-5%. The invention also discloses application of the pearl shell whitening factor obtained by the method in preparation of an endothelin antagonist. The preparation method is simple in process, and the obtained pearl shell whitening factor is obvious in whitening effect.

Description

technical field [0001] The present application relates to a pearl oyster whitening factor and its preparation method, and the use of the pearl oyster whitening factor, in particular to a pearl oyster whitening factor freeze-dried powder and its preparation method, and the antagonism of the pearl oyster whitening factor on endothelin. Background technique [0002] Whitening is the most concerned skin care demand of Asians. According to the research on the mechanism of skin whitening, inhibiting endothelin to stimulate cells to secrete melanin and inhibiting tyrosinase activity are two key links in the whitening effect. Therefore, many research institutes at home and abroad carry out the search for endothelin antagonists. For example, the patent (application number: 200610030123.5, invention name: enzyme-mediated mixed peptide of natural protein with endothelin antagonism) lists the preparation of many related protein polypeptides. But this patent ignores a lot of low-value ha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P21/06C07K2/00A61K8/98A61K8/64A61Q19/02
CPCC12P21/06C07K2/00A61K8/987A61K8/64A61Q19/02A61K8/98C07K4/12
Inventor 王菁杨安全张丽华莫家欢谢敏
Owner OSMUN BIOLOGICAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com