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Yeast culture, compound lactobacillus preparation and preparation methods thereof

A technology of yeast culture and compound lactic acid bacteria, which is applied in the field of lactic acid bacteria preparations, can solve the problems of unqualified hygiene indicators and failure to provide active ingredients in animal organisms, and achieve the effects of promoting food intake, improving the gastrointestinal environment, and reducing the use

Pending Publication Date: 2020-11-10
XIAMEN HUIYING ANIMAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These carriers cannot provide active ingredients to the animal body, and most of the health indicators are unqualified

Method used

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  • Yeast culture, compound lactobacillus preparation and preparation methods thereof
  • Yeast culture, compound lactobacillus preparation and preparation methods thereof
  • Yeast culture, compound lactobacillus preparation and preparation methods thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: lactic acid bacteria antibacterial test

[0034]The antibacterial test adopts the tube-and-dish method, fermenting and cultivating the respective lactic acid bacteria for 48 hours, passing the bacterial solution at 10,000 r / min, centrifuging for 10 minutes, taking the supernatant, and placing it in a 4°C refrigerator for later use. Take 1mL of pathogenic bacteria suspension and add it to 100mL MRS agar medium at 50°C and shake well (the final bacterial concentration is 10 5 cfu mL -1 ), pour it into a petri dish (thickness is about 3mm), put the Oxford cup on the flat plate after cooling and solidification, add 0.20mL supernatant to each Oxford cup, place it at 37°C for 24h, and observe whether there is inhibition Bacterial circles were formed, and the diameter of the inhibition zone was measured with a vernier caliper.

[0035] Table 1 Lactic acid bacteria inhibit the result table of pathogenic bacteria (24h)

[0036]

[0037] Inhibition zone diamet...

Embodiment 2

[0039] Embodiment 2 A kind of preparation of yeast culture and compound lactic acid bacteria preparation

[0040] (1) Activation of original strains: Inoculate Saccharomyces cerevisiae and Candida utilis into sterilized YPD slant medium for aerobic culture until colonies grow to obtain respective activated slant strains; YPD slant medium contains Glucose 20g, peptone 20g, yeast extract 10g, chloramphenicol 0.1g, agar powder 20g and distilled water 1L, pH natural;

[0041] (2) Preparation of primary seed liquid: Inoculate the Saccharomyces cerevisiae and Candida utilis slant strains obtained in step (1) into sterilized liquid YPD medium, and cultivate them aerobically at 33°C for 24h to obtain Yeast original liquid; liquid YPD medium contains 20g of glucose, 20g of peptone, 10g of yeast extract, 0.1g of chloramphenicol, 1L of distilled water, and the pH is natural;

[0042] (3) Preparation of secondary seed liquid: the original bacterial liquid of Saccharomyces cerevisiae and ...

Embodiment 3

[0049] Application of embodiment 3 compound lactic acid bacteria preparation in weaned piglets

[0050] Select about 80 weaned piglets about 30 days old, with similar body weight and healthy. They were randomly divided into 4 groups, 20 in each group, male and female in half. Among them, 3 groups were added the sample products of Example 1, Comparative Example 1 and Comparative Example 2 in the normal diet at a ratio of 1%, and one group was only fed the normal diet as a blank control group. The 4 groups were managed in the same way, and the test days were 30 days, and relevant data such as body weight, feed intake and diarrhea rate were observed and recorded.

[0051] Table 2 Test piglet production performance data

[0052]

[0053]

[0054] Note: No piglets died during the experiment

[0055] According to the data analysis in Table 2, it can be known that the feed-to-meat ratio of piglets can be improved and the diarrhea rate can be obviously reduced in Example 1. ...

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Abstract

The invention discloses a yeast culture. The yeast culture is prepared as follows: after saccharomyces cerevisiae and candida utilis are subjected to liquid-state fermentation culture, inoculation forsolid-state fermentation is carried out, and then drying, crushing and packaging are performed. The invention also discloses a compound lactobacillus preparation prepared by compounding the yeast culture as a carrier with lactobacillus plantarum, bacillus coagulans and lactobacillus rhamnosus, and the three lactobacillus powder raw materials are all subjected to thallus coating protection treatment. The compound lactobacillus preparation can be used for reducing or replacing antibiotics, can not only inhibit the growth and propagation of pathogenic bacteria in intestinal tracts of animals soas to reduce or prevent bacterial diseases, but also promote the digestion and absorption of nutrient substances of feed, improve the utilization rate of the feed, promote ingestion and enhance the immunity so as to promote growth of the animals and improve the production performance.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation technology and lactic acid bacteria preparation, in particular to a solid-state fermentation product of yeast and a method for preparing a probiotic preparation by using the product as a carrier to compound lactic acid bacteria. Background technique [0002] Most of the prevention or treatment of bacterial diseases in farmed animals uses chemical agents and antibiotics. These drugs not only pollute the environment, but also may cause drug residues and cause drug resistance of pathogenic microorganisms. In the breeding industry, with the widespread use of antibiotics and chemical fungicides, a series of disadvantages have gradually been brought about, such as the emergence of bacterial resistance, drug residues, damage to the normal intestinal flora, and damage to the environment. According to the Announcement No. 194 of the Ministry of Agriculture and Rural Affairs, antibiotics will...

Claims

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Application Information

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IPC IPC(8): A23K10/16A23K10/18A23K40/30C12N1/18C12N1/16C12N1/20C12N11/10C12N11/04C12R1/865C12R1/72C12R1/25C12R1/07C12R1/225
CPCA23K10/16A23K10/18A23K40/30C12N1/18C12N1/16C12N1/20C12N11/10C12N11/04
Inventor 林松泉林章秀乔欣君曾海燕黄榕
Owner XIAMEN HUIYING ANIMAL TECH
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