Preparation method and application of protein oligopeptide rich in desmosine and isodesmosine

A technology of isochain and oligopeptide, applied in the field of preparation of protein oligopeptide, can solve the problems of low bioavailability, affecting product quality, poor digestion and absorption, etc., so as to improve skin photoaging, improve product quality, easily absorbed effect

Pending Publication Date: 2020-11-10
安徽盛美诺生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fat content is high, the human body digests and absorbs poorly, and the bioavailability is not high
The currently reported elastin peptides prepared by enzymatic hydrolysis have been proved by experiments to improve the condition of skin and blood vessels, but these processes either use volatile organic solvents, or the enzymes used are non-food enzymes, which are difficult to convert
And elastin is not separated from collagen, glycoprotein, globulin, etc. Before such stable elastin is hydrolyzed, collagen, glycoprotein, globulin, etc. that coexist with it have been deeply hydrolyzed into oligopeptides, free amino acids, Even produce nitrogen-containing substances with small molecules, affecting product quality

Method used

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  • Preparation method and application of protein oligopeptide rich in desmosine and isodesmosine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1 is rich in the bovine arterin oligopeptide of DES and IDS

[0045] Take 2.03kg bovine aortic tube with muscle removed, break it into small pieces of about 0.5cm, add 4L 0.5% NaHCO 3 Solution, stirred and soaked for 1.5h, filtered and washed with water. Add 5L of water, adjust the pH value to 2.5, keep warm at 78°C for 2h, filter and wash with water. Add 4L of water to the filter residue, adjust the pH value to 6.8, add 0.1% papain, keep warm at 45°C for 2h, filter and wash with water. Add 6L of water to the remaining powder, and grind to 100 mesh in a colloid mill. Adjust the pH of the slurry to 8.0, add 6 g of alkaline protease and 1.5 g of trypsin, and perform enzymatic hydrolysis at a temperature of 55° C. and a pH of 7.8 for 6 hours. After the incubation, heat the enzymolysis solution to 85°C for 30 minutes to inactivate the enzyme, centrifuge at 4500 r / min for 20 minutes, and pass the supernatant through 10 μm and 0.45 μm filter membranes. The obta...

Embodiment 2

[0050] Embodiment 2 is rich in the bovine ligament protein oligopeptide of DES and IDS

[0051] Take 3.5kg of bovine neck ligament with muscle removed, break it into small pieces of about 0.5cm, add 7L 0.5% NaHCO 3 Solution, stirred and soaked for 0.5h, filtered and washed with water. Add 10L of water, adjust the pH value to 3.0, keep warm at 85°C for 2h, filter and wash with water. Add 15 L of water to the remaining powder, adjust the pH to neutral, and grind to 100 mesh with a colloid mill. Continue to adjust the pH value of the slurry to 8.3, add 12 g of alkaline protease and 3 g of trypsin, and incubate at a temperature of 60° C. and a pH of 8.0 for 5 hours. After the incubation, the enzymolysis solution was heated to 95°C for 15 minutes to inactivate the enzyme, centrifuged at 4500 r / min for 20 minutes, and the supernatant was passed through 10 μm and 0.45 μm filter membranes. The continuous constant volume diafiltration of the permeate passes through the nanofiltratio...

Embodiment 3

[0053] Embodiment 3 is rich in the porcine arterial protein oligopeptide of DES and IDS

[0054] Take 3.2kg of porcine arterial tube with muscle removed, break it into small pieces of about 0.5cm, add 7L of 0.5% NaHCO 3 Solution, stirred and soaked for 2h, filtered and washed with water. Add 7L of water, adjust the pH value to 2.2, keep warm at 85°C for 2h, filter and wash with water. Add 7L of water to the minced meat, adjust the pH to 7.2, add 6.5g of special enzyme for hydrolyzing collagen, keep warm at 60°C for 2h, filter and wash with water. 12L of water was added to the retentate, and the colloid mill was ground to 100 mesh. Adjust the pH of the slurry to 8.7, add 10 g of alkaline protease and 2.5 g of trypsin, and incubate at a temperature of 60° C. and a pH of 8.5 for 5 hours. After the incubation, the enzymatic solution was heated to 90°C for 15 minutes to inactivate the enzyme, centrifuged at 4500 r / min for 20 minutes, and the supernatant was passed through 10 μm ...

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Abstract

The invention relates to the technical field of polypeptide extraction, in particular to a preparation method and application of protein oligopeptide rich in desmosine and isodesmosine. The inventionprovides the preparation method of the protein oligopeptide. The method comprises the following steps of sequentially carrying out alkali liquor soaking and acid liquor soaking on to-be-extracted tissue, performing grinding until the granularity is 100-200 meshes, carrying out enzymolysis by using alkaline protease and pancreatin, carrying out enzyme deactivation and centrifugation to obtain supernatant, and carrying out fine filtration and nanofiltration to obtain a solution containing the protein oligopeptide, wherein the to-be-extracted tissue is the arterial canal, the ligament, the lung,the fascia, the cartilage or the eggshell membrane. The extracted protein oligopeptide is rich in desmosine and isodesmosine, and the polypeptide with the molecular weight less than 1,000 Da accountsfor 50%-85% of the total polypeptide mass. According to the method, alkali liquor and acid liquor are used for degreasing and impure protein removing, so that residues and pollution of an organic solvent are avoided, operation is easy, and the extraction efficiency is high.

Description

technical field [0001] The invention relates to the technical field of polypeptide extraction, in particular to a preparation method and application of protein oligopeptides rich in desmosin and isodesmosin. Background technique [0002] The skin is the largest organ of the human body, covering the whole body, with functions such as absorption, secretion, excretion, sensation, and participation in metabolism. It is also an important barrier and defense organ. Skin aging seriously affects people's health and beauty, and is a hot issue that people generally pay attention to and study. Among them, skin photoaging is the structural and functional changes caused by repeated exposure to ultraviolet rays for a long time. The main clinical manifestations of skin aging are rough skin, loose skin, sagging, and even tumors. The pathological manifestations are uneven thickness of the epidermis, flattening of the epidermis-dermis interface, telangiectasia, collagen fiber breakage and d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/18A23L33/175A23J3/34A23J3/04A23J1/04A23J1/02A61K8/64A61K38/01A61P17/18A61Q19/00
CPCA23L33/18A23L33/175A23J3/341A23J3/04A23J1/04A23J1/02A61K38/012A61K8/64A61Q19/00A61P17/18A23V2002/00A23V2200/30A23V2200/318A23V2250/06A23V2250/55A23V2300/14A23V2300/38
Inventor 杨红菊
Owner 安徽盛美诺生物技术有限公司
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