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A double-crosslinked multifunctional hydrogel dressing and its preparation and use

A double cross-linking, hydrogel technology, applied in the field of biological applications, can solve the problem of minimal wound healing effect on wound infection, achieve excellent bacteriostatic effect, not easy to rupture, and reduce the effect of β-folding

Active Publication Date: 2022-04-12
WENZHOU INST UNIV OF CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the existing silk fibroin gel is used as a skin dressing, it only acts as a carrier, and it has little effect on the wound healing of wound infection.

Method used

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  • A double-crosslinked multifunctional hydrogel dressing and its preparation and use
  • A double-crosslinked multifunctional hydrogel dressing and its preparation and use
  • A double-crosslinked multifunctional hydrogel dressing and its preparation and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: preparation SF / Hb / Ga hydrogel

[0027] Step 1: Cut silkworm cocoons into small pieces, boil them in 0.02 M sodium carbonate solution at 98°C for 1 h, repeat the degumming process twice, soak and wash in double distilled water for 5 times; the degummed silk fibroin is dissolved at 65°C in 9.3 M lithium bromide solution for 2 hours, adjust silk fibroin to 10% mass percent concentration; dialyze with 1M borate buffer solution with pH=9 for 1 day, continue dialysis with double distilled water for 3 days, and concentrate at low temperature to obtain 10% silk fibroin aqueous solution.

[0028] Step 2, take the silk fibroin aqueous solution that the mass percentage concentration is 10%, add the Ga(NO3) of 0.5mg / mL 3, described silk fibroin aqueous solution and Ga(NO3) 3 The volume ratio is 10:1, forming SF / Ga prefabricated gel;

[0029] Step 3. Take the hemoglobin aqueous solution with a mass percentage concentration of 2%, mix it with the SF / Ga prefabricated ge...

Embodiment 2

[0036] Embodiment 2: SF / Hb / Ga hydrogel, SF / Hb hydrogel and SF / Ga hydrogel antibacterial experiment

[0037] Take 1mL of SF aqueous solution, SF / Hb / Ga hydrogel, SF / Hb hydrogel and SF / Ga hydrogel respectively, add 5mL of Pseudomonas aeruginosa bacteria in logarithmic growth phase, and culture overnight on a shaking table at 37 °C. Take the upper layer of bacterial liquid to smear the plate, count the number of colonies after 6h, and the results are as follows: figure 2 As shown, the SF / Ga hydrogel contains gallium ions, which can play an antibacterial effect, the antibacterial effect of the pure SF / Hb hydrogel is not significant, and the antibacterial effect of the SF / Hb / Ga double cross-linked hydrogel The effect is obviously better than that of SF / Ga physically cross-linked gel. SF / Hb / Ga double cross-linked hydrogel has a synergistic effect on the antibacterial performance of gallium ions and hemoglobin under the double cross-linking effect of gallium ions and hemoglobin.

Embodiment 3

[0038] Embodiment 3: SF / Hb / Ga hydrogel, SF / Hb hydrogel and SF / Ga hydrogel mechanical property experiment

[0039] The same volume of SF / Hb / Ga hydrogel, SF / Hb hydrogel and SF / Ga hydrogel were taken respectively to test the mechanical experiment through the rheometer. The mechanical test results are as follows: image 3As shown in the figure; it can be seen that the gel film obtained by the present invention has good elastic modulus (G') and viscous modulus (G"), and the mechanical properties of the double cross-linked SF / Hb / Ga hydrogel are significantly better than that of a single Physically cross-linked SF / Ga hydrogel and enzymatically cross-linked SF / Hb hydrogel make the SF / Hb / Ga hydrogel more durable and less prone to breakage when used as a skin dressing.

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Abstract

The invention relates to a double-crosslinked multifunctional hydrogel dressing and its preparation and application. The hydrogel mainly includes three components: silk fibroin, hemoglobin, and gallium nitrate, and the gallium nitrate contains trivalent metal ions. It can form a physical cross-linked pregel with negatively charged SF; hemoglobin has peroxidase properties, which can catalyze the tyrosine of silk fibroin to form an enzyme cross-linked hydrogel, and the prepared hydrogel is in the presence of hemoglobin and gallium ions The synergistic effect has excellent antibacterial effect, and through double cross-linking, the hydrogel has good mechanical properties, and has outstanding application prospects in the direction of infected wound healing dressings.

Description

technical field [0001] The invention relates to the technical field of biological applications, in particular to a double-crosslinked multifunctional hydrogel dressing and its preparation and use. Background technique [0002] In recent years, the research and application of silk has extended from the traditional textile field to high-tech fields, such as optoelectronics and biomedical materials, especially as biomedical materials have made important progress. Silk fibroin is obtained by degumming silk. It is a natural structural protein without physiological activity. It is mainly composed of three simple amino acids: glycine, alanine and serine, which account for about 85% of the total protein. The degummed silk has good biocompatibility and mechanical properties, and has been widely used as skin dressings. [0003] Chronic wounds, most commonly including venous or arterial ulcers, pressure sores, and diabetic ulcers, are unable to restore anatomical and functional integr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L26/00C08J3/075C08J3/24C08L89/00C08K3/28C07K14/435C07K1/14
CPCA61L26/008A61L26/0047A61L26/0004A61L26/0066A61L26/0061C08J3/075C08J3/246C07K14/43586A61L2300/102A61L2300/404A61L2300/252A61L2300/45C08J2389/00C08J2489/00C08K3/28C08L89/00
Inventor 钱宇娜沈建良
Owner WENZHOU INST UNIV OF CHINESE ACAD OF SCI