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Serum-free culture medium for large-scale production of virus vectors as well as preparation method and use of serum-free culture medium

A serum-free medium and virus production technology, applied in biochemical equipment and methods, virus/bacteriophage, culture process, etc., can solve the problem of low virus recovery rate, achieve easy storage, reduce burden and cost, and ensure growth status and the effect of toxicity

Inactive Publication Date: 2020-11-24
SHENZHEN PREGENE BIOPHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The main purpose of the present invention is to provide a serum-free culture medium for large-scale production of viral vectors, a preparation method and its use, so as to solve the technical problem of low recovery rate of virus cultured in traditional medium

Method used

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  • Serum-free culture medium for large-scale production of virus vectors as well as preparation method and use of serum-free culture medium
  • Serum-free culture medium for large-scale production of virus vectors as well as preparation method and use of serum-free culture medium
  • Serum-free culture medium for large-scale production of virus vectors as well as preparation method and use of serum-free culture medium

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preparation example Construction

[0027] The present invention also provides a method for preparing a serum-free medium for large-scale production of viral vectors as described above, which is characterized in that it comprises the following steps:

[0028] Weigh each component in proportion, dissolve in double distilled water, adjust the pH value, filter and sterilize to obtain the serum-free medium for large-scale production of virus vectors;

[0029] Save for later use.

[0030] Optionally, the range of the pH value is 7.0-7.4. For example but not limited to, the pH value of the serum-free medium for the large-scale production of viral vectors is 7.2.

[0031] Optionally, the specific condition of the preservation is: the serum-free culture for the large-scale production of the virus vector is preserved at 2-8°C. For example but not limited to, the serum-free culture for large-scale production of viral vectors is based on preservation at 4°C.

[0032] The present invention also provides an application of...

Embodiment 1

[0035] The preparation of the serum-free medium of embodiment 1 large-scale production virus vector:

[0036] S1. Weigh each component of the corresponding serum-free medium, each component is as follows: glycine 45mg / L, L-alanine 55mg / L, L-valine 95mg / L, L-leucine 230mg / L, L-isoleucine 125mg / L, L-methionine 55mg / L, L-proline 230mg / L, L-tryptophan 60mg / L, L-serine 410mg / L, L -Tyrosine 145mg / L, L-cysteine ​​25mg / L, L-phenylalanine 355mg / L, L-asparagine 35mg / L, L-aspartic acid 40mg / L, L-glutamine Aminoamide 1000mg / L, L-threonine 225mg / L, L-glutamic acid 80mg / L, L-lysine 245mg / L, L-arginine 75mg / L, L-histidine 60mg / L , vitamin D2 0.8mg / L, vitamin B2 0.08mg / L, vitamin B1 0.06mg / L, vitamin B6 0.4mg / L, vitamin A 0.6mg / L, vitamin E 0.035mg / L, vitamin B12 45mg / L, vitamin C12mg / L, choline chloride 3.5mg / L, folic acid 0.65mg / L, anhydrous magnesium sulfate 65mg / L, potassium chloride 500mg / L, sodium bicarbonate 1200mg / L, sodium chloride 8500mg / L, hydrogen phosphate Disodium 800mg / L, fe...

Embodiment 2

[0039] The preparation of the serum-free medium of embodiment 2 large-scale production virus vector:

[0040]S1. Weigh each component of the corresponding serum-free medium, each component is as follows: glycine 40mg / L, L-alanine 50mg / L, L-valine 90mg / L, L-leucine 200mg / L, L-isoleucine 100mg / L, L-methionine 50mg / L, L-proline 200mg / L, L-tryptophan 50mg / L, L-serine 400mg / L, L -Tyrosine 120mg / L, L-cysteine ​​20mg / L, L-phenylalanine 300mg / L, L-asparagine 30mg / L, L-aspartic acid 35mg / L, L-glutamine Aminoamide 800mg / L, L-threonine 200mg / L, L-glutamic acid 60mg / L, L-lysine 200mg / L, L-arginine 60mg / L, L-histidine 50mg / L , vitamin D2 0.6mg / L, vitamin B2 0.06mg / L, vitamin B1 0.04mg / L, vitamin B6 0.3mg / L, vitamin A0.5mg / L, vitamin E 0.030mg / L, vitamin B12 40mg / L, vitamin C10mg / L, choline chloride 3.0mg / L, folic acid 0.55mg / L, anhydrous magnesium sulfate 60mg / L, potassium chloride 480mg / L, sodium bicarbonate 1000mg / L, sodium chloride 8000mg / L, hydrogen phosphate Disodium 700mg / L, ferri...

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Abstract

The invention discloses a serum-free culture medium for large-scale production of virus vectors as well as a preparation method and use of the serum-free culture medium. According to the technical scheme of the invention, components of the serum-free culture medium are designed, so that the technical problem of low recovery rate of viruses cultured by virtue of a traditional culture medium is solved.

Description

technical field [0001] The invention relates to the technical field of biological products, in particular to a serum-free medium for large-scale production of viral vectors, a preparation method and use thereof. Background technique [0002] At present, cell culture technology has become a common method for industrial production of biologically active substances, vaccines, carriers, such as monoclonal antibodies, pharmaceutical proteins, genetic engineering drugs, etc. Cell culture technology is closely related to genetic engineering technology, enzyme engineering technology and fermentation engineering technology It plays a key and central role in the development of the entire biotechnology industry. In addition, the improvement of cell culture technology has promoted the development of protein expression and purification technology and virus culture technology. Judging from the current development trend, cell suspension culture and serum-free culture are the development d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073C12N7/00
CPCC12N5/0603C12N5/0686C12N7/00C12N2500/10C12N2500/12C12N2500/16C12N2500/20C12N2500/22C12N2500/30C12N2500/32C12N2500/33C12N2500/34C12N2500/38C12N2500/40C12N2500/44C12N2500/50C12N2500/90C12N2501/999
Inventor 李华秀梁锦华李海玲宋宗培栗红建包朝乐萌王玲
Owner SHENZHEN PREGENE BIOPHARMA CO LTD
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