Preparation method of porous hollow calcium carbonate drug-loaded microspheres

A technology of drug-loaded microspheres and calcium carbonate, applied in the field of biomedical engineering, can solve problems such as poor controllability, small holes, and difficulty in entering porous CaCO, and achieve the effects of low cost, simple preparation process, and high drug-loading capacity.

Inactive Publication Date: 2020-11-27
TAIYUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, CaCO prepared using the above template 3 The controllability of the structure is poor, and the holes obtained are too small, and it is difficult for some protein drugs with large molecular weight to enter the porous CaCO 3 middle

Method used

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  • Preparation method of porous hollow calcium carbonate drug-loaded microspheres
  • Preparation method of porous hollow calcium carbonate drug-loaded microspheres
  • Preparation method of porous hollow calcium carbonate drug-loaded microspheres

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Add the yeast into 5 mL of liquid medium, put it into a constant temperature shaker set at 180 rpm and 37°C for 16 h, and wait for the bacteria to grow to 10 6After cfu / mL concentration, centrifuge at 3000 rpm for 5 min, remove the supernatant, take the precipitate and place it in 0.9% NaCl solution for resuspension; add 20 mL of polydiene dimethyl at a concentration of 1.0 g / mL to the above bacteria solution Ammonium chloride solution (PDDA) solution, shake in a constant temperature shaker at 37°C at 180 rpm for 15 minutes, then centrifuge at 6000 rpm for 5 minutes, then remove the supernatant, add 20 mL of polystyrene sulfonate with a concentration of 1.0 g / mL Shake the acid solution at 180 rpm for 15 min in a constant temperature shaker at 37°C, and then centrifuge at 6000 rpm for 5 min. Repeat this step 4 times. The strain cells treated above were transferred into a beaker, and 100 mL of 0.33 M Ca 2+ The solution was placed in a water bath under magnetic force at 3...

Embodiment 2

[0031] Add the above-mentioned yeasts into 5 mL of liquid medium, put them into a constant temperature shaker set at 180 rpm and 37°C for 16, and wait for the bacteria to grow to 10 6 After cfu / mL concentration, centrifuge at 3000 rpm for 5 min, remove the supernatant, take the precipitate and place it in 0.9% NaCl solution for resuspension; add 20 mL of polydiene dimethyl at a concentration of 2.0 g / mL to the above bacteria solution Ammonium chloride solution (PDDA) solution, shake in a constant temperature shaker at 37°C at 180 rpm for 15 min, then centrifuge at 6000 rpm for 5 min, then remove the supernatant, add 20 mL polystyrene sulfonate with a concentration of 2.0 g / mL Acid PSS solution, shake well and disperse, shake in a constant temperature shaker at 37°C at 180 rpm for 15 min, then centrifuge at 6000 rpm for 5 min, repeat this step 4-6 times. The strain cells treated above were transferred into a beaker, and 100 mL of 0.5 M Ca 2+ The solution was placed in a water...

Embodiment 3

[0033] Add the above-mentioned Escherichia coli to 5 mL of liquid medium, put it into a constant temperature shaker set at 180 rpm and 37 °C for 16 h, and wait for the bacteria to grow to 10 6 After cfu / mL concentration, centrifuge at 3000 rpm for 5 min, remove the supernatant, take the precipitate and place it in 0.9% NaCl solution for resuspension; add 20 mL of polydiene dimethyl at a concentration of 1.0 g / mL to the above bacteria solution Ammonium chloride solution (PDDA) solution, shake in a constant temperature shaker at 37°C at 180 rpm for 15 minutes, then centrifuge at 6000 rpm for 5 minutes, then remove the supernatant, add 20 mL of polystyrene sulfonate with a concentration of 1.0 g / mL Acid PSS solution, shake well and disperse, shake in a constant temperature shaker at 37°C at 180 rpm for 15 min, then centrifuge at 6000 rpm for 5 min, repeat this step 4-6 times. The above-mentioned treated strain cells were transferred to a beaker, and 100 mL of 0.33M Ca 2+ The so...

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Abstract

A preparation method of porous hollow calcium carbonate drug-loaded microspheres comprises the following steps of: (1) culturing of microbial cells: adding strains into a liquid culture medium, takingprecipitates, and putting the precipitates into a NaCl solution for resuspension for later use; (2) preparation of a strain surface layer-by-layer self-assembly coating: alternately adding the strains into a polydiene dimethyl ammonium chloride solution and a polystyrene sulfonic acid solution to obtain strain cells coated with polyelectrolyte on the surface; (3) calcium salt pretreatment: addingthe strain cells into a Ca<2+> solution, magnetically stirring and fully and uniformly mixing; (4) preparation of calcium carbonate shells: dropwise adding CO3<2-> solution with the same amount intothe solution, and magnetically stirring for 16 hours; and repeatedly cleaning with pure water, and then drying and grinding, thus obtaining nano calcium carbonate shells on the surfaces of the straincells. and (5) preparation of the porous hollow calcium carbonate microspheres: putting the nano calcium carbonate shell particles into a tubular furnace, and removing strain cells in the inner layerto obtain the hollow porous calcium carbonate microspheres. The method is low in cost and simple in preparation process.

Description

technical field [0001] The invention belongs to drug slow-release carrier, relates to a preparation method of porous hollow calcium carbonate drug-loaded microspheres, belongs to the field of biomedical engineering, and can be used as a drug slow-release carrier material in biological materials. Various oral drug carriers can be imparted with pH sensitivity and biodegradability. Background technique [0002] In recent decades, drug carriers, such as microcapsules, nanotubes, and hollow nanoparticles, have attracted considerable attention in tumor therapy because they can passively spill into tumor tissues and accumulate, thereby effectively reducing side effects and enhancing drug efficacy. Pharmacokinetic properties, prolonged circulation half-life, improved utilization, safety and efficacy of drugs. So far, such as liposomes, dendrimers, polymer nanoparticles, calcium phosphate, silicon oxide, zinc oxide and calcium carbonate (CaCO 3 ), which have been developed for drug...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01F11/18A61K9/16A61K31/704A61K47/02A61P35/00C12N1/20C12N1/18C12R1/19C12R1/445
CPCC01F11/18C01F11/185C12N1/20C12N1/18A61K9/1611A61K31/704A61P35/00C01P2004/03C01P2004/61C01P2004/34
Inventor 魏延苏慧黄棣胡银春赵丽琴
Owner TAIYUAN UNIV OF TECH
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