A kind of preparation method of soybean 7s antioxidant selenopeptide

An anti-oxidation and soybean technology, which is applied in the preparation methods of peptides, chemical instruments and methods, and food ingredients as antioxidants, etc., can solve the problems of less selenium-containing components and lack of antioxidant activity, and achieve high selenium content and prevent oxidation. Related diseases, high degree of hydrolysis effect

Active Publication Date: 2022-04-12
石天朝(恩施)生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a preparation method of soybean 7S antioxidative selenopeptide. In order to solve the problems of less selenium-containing components and lack of antioxidant activity in the prior art, the double-enzyme enzymolysis process provided by the invention can improve the enzyme The amount of peptide released during the hydrolysis process, and the antioxidant activity of selenopeptides was significantly enhanced

Method used

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  • A kind of preparation method of soybean 7s antioxidant selenopeptide
  • A kind of preparation method of soybean 7s antioxidant selenopeptide
  • A kind of preparation method of soybean 7s antioxidant selenopeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1: the screening test of hydrolase

[0049] 1) Selenium-enriched fertilizer (0.1g Na 2 SeO 3 and 2.8g of sodium alginate dissolved in 1L of water) were sprayed on the leaves of soybeans at the full flowering stage. The fertilization cycle was four weeks, and fertilization was once every two days. After the soybeans matured, they were harvested and dried in the sun for later use.

[0050] 2) Take selenium-enriched soybeans, grind them through a 200-mesh sieve, take 30g of soybean powder and add 1000mL of petroleum ether, stir and extract at room temperature for 2 hours, let stand, remove petroleum ether after precipitation is complete, and obtain defatted soybean powder after precipitation and drying . Take 30g of defatted soybean powder, add 1000mL of distilled water, adjust the pH to 9.0 with 1mol / mL NaOH solution, centrifuge to get the supernatant after stirring for 2 hours, adjust the pH of the supernatant to 6.4 with 1mol / mL HCl solution, centrifuge aft...

Embodiment 2

[0054] Example 2: Addition order of protease

[0055] Take 3g of 7S protein, dissolve it in 100mL distilled water, and then carry out enzymatic hydrolysis in the order of neutral protease-alkaline protease and alkaline protease-neutral protease, the enzyme addition amount is 3% (w / w), and the enzymatic hydrolysis time 3h, enzymolysis temperature 50-60℃, boiling water bath 10min to terminate the reaction. The degree of hydrolysis of the enzymolyzate, the selenium content of the enzymolyzate, the DPPH clearance rate and the CAA EC were detected by the method of Example 1. 50 , the test results are shown in Table 1.

[0056] Table 1: Effect of enzymatic hydrolysis sequence on enzymatic hydrolysis effect

[0057] neutral-alkaline alkaline-neutral neutral alkaline Selenium content (μg / g) 19.721±0.892 a

[0058] The results showed that the degree of hydrolysis, selenium content and antioxidant activity of the product after double-enzyme hydrolysis were sig...

Embodiment 3

[0059] Embodiment 3: Enzymolysis condition optimization

[0060] 1) Effect of substrate concentration on enzymatic hydrolysis effect

[0061] Take 1-5g of 7S protein and add it to 100ml of distilled water, then add neutral protease and alkaline protease for enzymatic hydrolysis, the amount of enzyme added is 3% (w / w), the enzymatic hydrolysis time is 3h, and the enzymatic hydrolysis temperature is 50-60℃, The reaction was terminated in a boiling water bath for 10 min. Detect the degree of hydrolysis, DPPH clearance and CAAEC of the enzymolyzed solution by the method of Example 1 50 , the test results are as figure 2 shown.

[0062] The results showed that when the substrate concentration was 4%, the DPPH free radical scavenging rate and CAA EC of the hydrolyzate 50 reached the optimal value. Continuing to increase the substrate concentration, the hydrolysis effect becomes worse. Therefore, adding 4g of 7S protein to 100ml of water, that is, preparing a 4% protein soluti...

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Abstract

The invention discloses a preparation method of soybean 7S antioxidant selenopeptide, which comprises the following steps: (1) spraying selenium fertilizer on the leaves of soybeans from the full flowering stage, harvesting and drying soybeans after maturity to obtain selenium-enriched soybeans; 2) Grinding selenium-enriched soybeans and defatting to obtain defatted soybean powder, extracting 7S protein from the defatted soybean powder; (3) dissolving soybean 7S protein in water, then adding protease to the protein solution for enzymatic hydrolysis, inactivating and centrifuging to obtain supernatant; (4) filtering the supernatant with a hollow fiber ultrafiltration membrane, and freeze-drying the filtrate to obtain soybean 7S antioxidant selenopeptide. The soybean 7S antioxidant selenopeptide prepared by the invention has good antioxidant activity inside and outside the cell, and also has the advantages of high degree of hydrolysis and high selenium content.

Description

technical field [0001] The invention relates to a preparation method of soybean 7S antioxidant selenopeptide. Background technique [0002] Soybean is an important source of plant protein for humans and animal husbandry. Soybean protein is mainly composed of four components: 2S, 7S, 11S, and 15S. Among them, 7S protein accounts for about one-third of soybean protein. However, there are many studies on soybean 11S protein, but relatively few studies on soybean 7S protein. [0003] The trace element selenium is regarded as an important food-derived antioxidant. Its function of scavenging oxygen free radicals and preventing biofilm damage has been widely recognized. It functions as an oxidoreductase in most organisms, thereby protecting the body from oxidative damage. However, selenium levels in foods are relatively low. According to statistics released by the World Health Organization (WHO), about 1 billion people in the world suffer from selenium malnutrition. Studies ha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34A23L33/16A23L33/18A61K38/01A61P39/06
CPCC12P21/06A23L33/18A23L33/16A61K38/011A61P39/06A23V2002/00A23V2200/02A23V2250/1626A23V2250/55
Inventor 侯焘张婞何慧贾蕾
Owner 石天朝(恩施)生物科技有限公司
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