Adhesive repair-promoting hemostatic sponge and preparation method thereof

A technology of hemostatic sponge and hydroxysuccinimide ester, which is applied in the field of biomedical materials, can solve the problems of weak tissue adhesion, low mechanical strength of sponge, weak cross-linked network structure, etc., and achieve the effect of ensuring swelling degree

Active Publication Date: 2020-12-01
SHANDONG BRANDEN MEDICAL DEVICE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this invention still has the problems of weak cross-linked network structure, low mechanical strength of the sponge, weak adhesion to tissues, and decreased function of promoting repair.

Method used

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  • Adhesive repair-promoting hemostatic sponge and preparation method thereof
  • Adhesive repair-promoting hemostatic sponge and preparation method thereof
  • Adhesive repair-promoting hemostatic sponge and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 The mass fraction of 20% N-maleoylated gelatin (60-90KDa, degree of substitution 85-95%), 0.75% exosomes, 3% acrylate PEG-N hydroxysuccinimide Add ester (3.4KDa) and 0.015% N, N'-methylenebisacrylamide into purified water, stir at 100-200rpm until completely dissolved, and continue stirring for 20min. Add 1.0% maleic anhydride, 20% acrylic acid and 0.65% 2-hydroxy-2-methyl-1-[4-(2-hydroxyethoxy)phenyl]-1-propanone at 100-200rpm Stir at 100-200rpm until completely dissolved, then add sodium hydroxide solution to neutralize until the neutralization degree is 50% under stirring at 100-200rpm, pre-polymerize for 30min under a UV lamp at a power of 150W, a wavelength of 365nm, and atomize and spray 0.75% acrylic acid A mixture of N-hydroxysuccinimide ester and 0.010% 2-hydroxy-2-methyl-1-[4-(2-hydroxyethoxy)phenyl]-1-propanone, at a power of 150W, The wavelength is 365nm. After 15 minutes of polymerization under ultraviolet light, pre-freeze at -50°C for 4 hours. ...

Embodiment 2

[0051] Example 2 The mass fraction is 15% N-maleoylated gelatin (50-80KDa, substitution degree 85-95%), 0.5% exosomes, 2% acrylate PEG-N hydroxysuccinimide Add ester (2KDa) and 0.02% N, N'-methylenebisacrylamide into purified water, stir at 100-200rpm until completely dissolved, and continue stirring for 20min. Add 1.25% maleic anhydride, 25% acrylic acid and 0.8% 2-hydroxy-2-methyl-1-[4-(2-hydroxyethoxy)phenyl]-1-propanone at 100-200rpm Stir at 100-200rpm until completely dissolved, add sodium hydroxide solution and disodium hydrogen phosphate solution to neutralize to 60% under stirring at 100-200rpm, pre-polymerize under UV lamp for 40min with a power of 150W, a wavelength of 365nm, fog Spray the mixture of 0.5% N-hydroxysuccinimide acrylate and 0.007% 2-hydroxy-2-methyl-1-[4-(2-hydroxyethoxy)phenyl]-1-acetone , at a power of 150W, a wavelength of 365nm, after 10 minutes of polymerization under a UV lamp, pre-freeze at -50°C for 4 hours, and then vacuumize after the end, a...

Embodiment 3

[0052] Example 3 N-maleoylated gelatin (70-100KDa, substitution degree 85-95%) with a mass fraction of 25%, 1.0% exosomes, 4% acrylate PEG-N hydroxysuccinimide Add ester (5KDa) and 0.01% N, N'-methylenebisacrylamide into purified water, stir at 100-200rpm until completely dissolved, and continue stirring for 20min. Add 0.75% maleic anhydride, 15% acrylic acid and 0.5% 2-hydroxy-2-methyl-1-[4-(2-hydroxyethoxy)phenyl]-1-propanone at 100-200rpm Stir at 100-200rpm until completely dissolved, add sodium hydroxide solution and sodium bicarbonate solution to neutralize to 40% under stirring at 100-200rpm, pre-polymerize under UV lamp for 20min under power of 150W, wavelength of 365nm, and atomize Spray a mixture of 1.0% N-hydroxysuccinimide acrylate and 0.013% 2-hydroxy-2-methyl-1-[4-(2-hydroxyethoxy)phenyl]-1-acetone, After polymerization at 150W, wavelength 365nm, under UV light for 20min, pre-freeze at -50°C for 4 hours, then vacuumize after completion, the vacuum degree should b...

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Abstract

The invention discloses an adhesive repair-promoting hemostatic sponge and a preparation method thereof. The hemostatic sponge is prepared according to the following steps: subjecting N-maleinized gelatin, exosome, acrylate-PEG-N-hydroxysuccinimide ester, N,N'-methylenebisacrylamide to full reaction, adding maleic anhydride, acrylic acid and a first part of photoinitiator, performing neutralizingwith a neutralizer, carrying out prepolymerization, adding a mixed solution of acrylic acid N-hydroxysuccinimide ester and a second part of photoinitiator, fully performing polymerizing, and carryingout programmed freeze-drying. The hemostatic sponge prepared by the invention not only can quickly absorb blood or tissue fluid to form gel, form mechanical compression on damaged blood vessels on the surface of a cavity and promote hemostasis, but also can be adhered to the surface of the cavity, so that displacement is prevented while hemostasis is further promoted. The hemostatic sponge disclosed by the invention can slowly release gelatin and exosome, and has a long-acting repair promoting function. Therefore, the hemostatic sponge has the effects of quickly and efficiently stopping bleeding, achieving adhering and promoting repair.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and relates to a hemostatic sponge capable of adhering and promoting repair and a preparation method thereof. The medical hemostatic sponge of the present application has the functions of being able to expand and quickly stop bleeding, and at the same time having the functions of being able to adhere, preventing displacement and promoting repair. Background technique [0002] In the first aid of sudden accidents, surgery and in war, 50% of the deaths are caused by massive bleeding. Some traditional hemostatic materials, such as hemostatic gauze, hemostatic bandage, hemostatic cotton yarn, etc., have limited hemostatic ability, and the hemostatic effect is not very satisfactory. Therefore, the development of highly efficient and quickly absorbable hemostatic materials and products, which can effectively stop bleeding within 1-2 minutes or even shorter after the bleeding occurs, has b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/10A61L24/00A61L24/04C08J9/28C08F285/00C08F220/36C08F2/48
CPCA61L24/0005A61L24/001A61L24/0036A61L24/046A61L24/104A61L2300/412A61L2300/602A61L2400/04C08F2/48C08F285/00C08J9/28C08J2201/0484C08J2351/00C08L71/02C08F220/36
Inventor 张海军袁坤山车超越张淑欣侯文博尹玉霞鲁守涛段翠海
Owner SHANDONG BRANDEN MEDICAL DEVICE
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