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A seawater spirulina-bacteria symbiosis system, construction method and application

A symbiotic system, spirulina technology, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of high cost, high cost and high cost of sludge treatment, and achieve good denitrification and phosphorus removal. The effect of improving metabolism and promoting bacterial growth

Active Publication Date: 2022-02-08
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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AI Technical Summary

Problems solved by technology

[0006] (1) At present, under the condition of high salinity, the biological treatment method generally applicable to urban sewage treatment greatly inhibits the microbial activity. The traditional activated sludge method cannot be directly used for the treatment of mariculture tail water, and can only It takes a long time to start up domesticated sludge; and due to the low carbon / nitrogen characteristics of mariculture tail water, it is necessary to add a large amount of carbon source to the culture system to efficiently treat tail water, which greatly increases the treatment of mariculture tail water cost
[0007] (2) At present, the biological treatment method generally applicable to urban sewage treatment removes phosphorus in the water body by discharging sludge, and the cost of subsequent sludge treatment is relatively high
If the addition of a large amount of carbon sources is not considered, it will be difficult to ensure the treatment efficiency of mariculture tail water, and it will be difficult to reflect the usefulness of domesticated sludge
On the other hand, the joint action of nitrifying bacteria and denitrifying bacteria can finally remove nitrogen from the water body in the form of nitrogen gas, while phosphorus can only be removed from the water body through the phosphorus-rich sludge formed by phosphorus accumulating bacteria. Phosphorus is removed from the water body in the form of sludge discharge, and the treatment of sludge takes a long time and high cost, which increases the treatment cost of mariculture tail water
[0009] The significance of solving the above problems and defects is: through the analysis of the difficulty of the above problems and defects, it is not difficult to see that there is a high difficulty in using the activated sludge method to treat the tail water of mariculture, and the high cost is the limit of the application of the activated sludge method. Therefore, there is an urgent need for a low-cost biological treatment method for mariculture tail water treatment, which can efficiently remove nitrogen and phosphorus nutrients from mariculture tail water without adding exogenous substances, and can The products of treating mariculture tail water are used in other fields, reducing treatment costs and developing downstream industries

Method used

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  • A seawater spirulina-bacteria symbiosis system, construction method and application
  • A seawater spirulina-bacteria symbiosis system, construction method and application
  • A seawater spirulina-bacteria symbiosis system, construction method and application

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Embodiment 1

[0060] In the present invention, seawater spirulina and Nitratireductor sp.strain ZYF759 are mixed and cultured to obtain a stable algae-bacteria symbiosis system. The inoculated biomass ratio of seawater spirulina and Nitratireductor sp.strain ZYF759 is 3:1;

[0061] Described seawater spirulina medium is seawater Zarrouk medium;

[0062] Described Nitratireductor sp.strain ZYF759 medium is 2216E liquid medium;

[0063] The mixed culture container is a 1000mL Erlenmeyer flask, and three parallel experiments are set for each group, and 500mL seawater Zarrouk medium is housed in the 1000mL Erlenmeyer flask;

[0064] The inoculation method is to centrifuge the seawater Spirulina culture medium and the Nitratireductor sp.strain ZYF759 culture medium separately, centrifuge at 5000 rpm for 5 minutes, discard the supernatant, resuspend and mix with fresh Zarrouk medium;

[0065] The temperature of the mixed culture was 30°C, and the light intensity was 60 μmol m -1 ·s -1 , the li...

Embodiment 2

[0071] In the present invention, seawater spirulina and Nitratireductor sp.strain ZYF759 are mixed and cultured to obtain a stable algae-bacteria symbiosis system. The inoculated biomass ratio of seawater spirulina and Nitratireductor sp.strain ZYF759 is 3:1;

[0072] Described seawater spirulina medium is seawater Zarrouk medium;

[0073] Described Nitratireductor sp.strain ZYF759 medium is 2216E liquid medium;

[0074] The mixed culture container is a 1000mL Erlenmeyer flask, and three parallel experiments are set for each group, and 500mL of simulated seawater aquaculture tail water is housed in the 1000mL Erlenmeyer flask;

[0075] The composition of the simulated mariculture tail water is: 132mg L -1 Glucose, 33.71mg·L -1 Ammonium chloride, 25.25mg·L -1 Potassium nitrate, 15.55mg·L -1 Potassium dihydrogen phosphate, 90mg·L -1 Magnesium sulfate, 14mg·L -1 Calcium chloride, 0.25mg·L -1 Cobalt chloride hexahydrate, 0.5mg L -1 Copper sulfate pentahydrate, 0.5mg L -1 ...

Embodiment 3

[0085]In the present invention, seawater spirulina and Microcellaalkaliphila, strain:JAM AC0309 are mixed and cultured to obtain a stable algae-bacteria symbiosis system, and the inoculated biomass ratio of seawater spirulina and Microcellaalkaliphila,strain:JAM AC0309 is 3:1;

[0086] Described seawater spirulina medium is seawater Zarrouk medium;

[0087] Described Microcellaalkaliphila, strain: JAM AC0309 medium is 2216E liquid medium;

[0088] The mixed culture container is a 1000mL Erlenmeyer flask, and three parallel experiments are set for each group, and 500mL seawater Zarrouk medium is housed in the 1000mL Erlenmeyer flask;

[0089] The inoculation method is to centrifuge the seawater Spirulina culture medium and Microcellaalkaliphila, strain:JAM AC0309 culture medium separately, centrifuge at 5000rpm for 5min, discard the supernatant, and use fresh Zarrouk medium to resuspend and inoculate;

[0090] The temperature of the mixed culture was 30°C, and the light intens...

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Abstract

The invention belongs to the technical field of mariculture tail water treatment, and discloses a seawater spirulina symbiosis system, construction method and application. The symbiosis system is co-cultivation of a single strain of seawater spirulina phycobacteria and seawater spirulina. It is self-isolated from seawater spirulina culture medium; the biomass ratio of seawater spirulina phycobacteria to seawater spirulina is 1:3 when inoculated. Inoculate a single strain of seawater spirulina phycobacterium and seawater spirulina according to the ratio of biomass 1:3; mixed culture in the medium; the culture conditions are culture temperature 30 ℃, light intensity 60 μmol m ‑1 ·s ‑1 , the light-to-dark ratio is 12:12, and the culture time is 10 days. The invention forms a stable symbiotic relationship between seawater spirulina-coalgae bacteria, and the addition of seawater spirulina can greatly promote the growth rate of seawater spirulina, accelerate the absorption of nutrients such as nitrogen and phosphorus in the water body, and indirectly accelerate the tail water of mariculture processing speed.

Description

technical field [0001] The invention belongs to the technical field of mariculture tail water treatment, and in particular relates to a seawater spirulina-interalgae symbiosis system, a construction method and an application. Background technique [0002] At present: my country is a big aquaculture country, and the output of marine aquaculture products accounts for a large proportion. Due to the complex composition and high salinity of mariculture tail water, it is difficult to treat it. Based on cost considerations, biological treatment has greater advantages than physical and chemical methods. Due to the inhibition of salinity on microorganisms, the traditional activated sludge method is not applicable. Therefore, there is an urgent need for an effective biological treatment method for mariculture tail water treatment. The co-treatment process of algae and bacteria has been proved to be effective in the treatment of aquatic product processing wastewater, seawater flushin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F103/20C02F103/22
CPCC12N1/20C02F3/341C02F2103/20C02F2103/005C02F2103/22
Inventor 崔鸿武汪鲁曲克明崔正国王斌丁任业
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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