Bifidobacterium pseudocatenulatum capable of relieving colitis and application thereof

A technology for bifidobacteria, colitis, applied in bifidobacteria, applications, bacteria used in food preparation, etc., can solve problems such as affecting the success of treatment, achieve weight loss, reduce disease activity index, broad market Foreground effect

Active Publication Date: 2020-12-22
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The currently used therapeutic drugs are: sulfasalazine salicylic acid preparations such as Edissa, mesalamine, etc.; corticosteroids commonly used are prednisone or dexamethasone, but long-term use of the above drugs may lead to hypertension, diabetes , osteoporosis and other diseases, thereby affecting the success of treatment

Method used

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  • Bifidobacterium pseudocatenulatum capable of relieving colitis and application thereof
  • Bifidobacterium pseudocatenulatum capable of relieving colitis and application thereof
  • Bifidobacterium pseudocatenulatum capable of relieving colitis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] Example 1: Screening, Identification, Culture, Observation and Preservation of Bifidobacterium pseudocatenulatum MY40C

[0092] 1. Screening

[0093] Take 1g of feces samples from healthy infants in Wuxi area, spread them in MRS solid medium (containing 10 μg / mL mupirocin antibiotic) after serial dilution, culture them in an anaerobic environment at 37°C for 72 hours, observe and record the colonies Morphology: Pick the colony with wet surface, bulge, and white yellowish color, streak it on the MRS solid medium, carry out purification culture under anaerobic conditions at 37°C, repeat this operation 3 times, and obtain a single colony after purification Pick a single colony and streak it on the MRS solid medium, culture it anaerobically at 37°C for 36 hours, and carry out Gram staining to the gained colony (Gram staining method refers to the textbook "Industrial Microbial Breeding" author: Zhuge Jian), Record the morphology of the colonies, and investigate the physiolo...

Embodiment 2

[0108] Embodiment 2: the preparation of pseudo small chain Bifidobacterium (Bifidobacterium pseudocatenulatum) MY40C bacterial suspension

[0109] (1) Dip the bacteria solution of Bifidobacterium pseudocatenulatum MY40C from the glycerol tube, streak on the MRS solid medium, culture at 37°C for 48 hours in an anaerobic environment, and obtain a single colony; pick a single colony for inoculation In the MRS liquid culture medium, culture at 37° C. for 48 hours under anaerobic environment for activation culture, repeat this operation 3 times, and obtain the activated bacterial liquid.

[0110] (2) Inoculate the activated bacterial liquid obtained in step (1) into the MRS liquid medium according to the inoculum size of 2% (v / v), cultivate at 37°C for 24 hours to obtain a fermentation liquid, and collect the bacteria by centrifugation of the fermentation liquid body, resuspend the cell body with physiological saline, and adjust the number of viable cells to 5×10 9 CFU / mL, made in...

Embodiment 3

[0111] Embodiment 3: Pseudo-small chain Bifidobacterium (Bifidobacterium pseudocatenulatum) MY40C alleviates the symptoms of colitis mice induced by DSS

[0112] Proceed as follows:

[0113] (1) Preparation of 2.5% DSS solution: Dextran sodium sulfate (DSS) was prepared into a 2.5% (w / v) DSS solution with sterilized tap water.

[0114] (2) 24 8-week-old healthy male C57BL / 6J mice were randomly divided into 3 groups, and the 3 groups were respectively named: blank control group (Control), model group (DSS), pseudo small chain Bifidobacterium ( Bifidobacterium pseudocatenulatum) MY40C intervention group (MY40C+DSS); 8 mice were used in each group, and the experimental scheme and treatment methods of mice in each group are shown in Table 4.

[0115] (3) Treat the blank control group (Control), the modeling group (DSS), and the Bifidobacterium pseudocatenulatum MY40C intervention group (MY40C+DSS);

[0116] Among them, the treatment method of the Bifidobacterium pseudocatenulatu...

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Abstract

The invention discloses bifidobacterium pseudocatenulatum capable of relieving colitis and application thereof, and belongs to the technical field of biology. The invention provides bifidobacterium pseudocatenulatum MY40C which has immunoregulation capacity and can improve an intestinal tract barrier and relieve colitis. By adopting the bifidobacterium pseudocatenulatum provided by the invention,the disease activity index of a mouse during DSS induced colitis can be remarkably reduced, and the weight loss and the colonic shortening can be relieved. Compared with a mouse in a model group, themouse in a bifidobacterium pseudocatenulatum MY40C intervention group has the advantages that the levels of MPO and COX-2 in colonic tissues are obviously reduced, and the tissue injury is obviously relieved.

Description

technical field [0001] The invention relates to a strain of pseudosmall chain bifidobacteria capable of relieving colitis and application thereof, belonging to the field of biotechnology. Background technique [0002] Ulcerative colitis is a diffuse inflammatory response characterized by intracrypt abscesses and infiltration of neutrophils, eosinophils, and plasma cells that attack the colonic lining. The most common symptoms of UC patients are diarrhea, rectal bleeding, tenesmus, mucus discharge, and abdominal pain. [0003] The etiology of ulcerative colitis is still unknown, and it is currently considered to be the result of the interaction between host response caused by foreign substances, gene and immune influence. [0004] Bloody diarrhea is the most common early symptom of ulcerative colitis. Other symptoms include abdominal pain, blood in the stool, weight loss, tenesmus, and vomiting in sequence. Occasionally the main manifestations are arthritis, iridocyclitis, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/745A61P1/00A23L33/135C12R1/01
CPCA61K35/745A61P1/00A23L33/135A23V2002/00C12R2001/01C12N1/205A23V2400/535A23V2200/32A23V2200/3204
Inventor 杨波陈卫陈洋陈海琴高鹤赵建新张灏
Owner JIANGNAN UNIV
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