A kind of Lactobacillus brevis 8-2b and its application

A technology of Lactobacillus brevis and bacteria agent, applied in the field of microbiology, can solve the problems of high cost, nutrient loss, low efficiency, etc., and achieve the effect of good development and application prospects

Active Publication Date: 2022-07-19
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of physical and chemical methods to inhibit bacteria has the disadvantages of high cost, low efficiency, and nutrient loss. Therefore, the use of efficient, non-toxic and harmless microorganisms to control the growth and toxin production of Aspergillus carbon black in food raw materials and processing has become a research hotspot.

Method used

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  • A kind of Lactobacillus brevis 8-2b and its application
  • A kind of Lactobacillus brevis 8-2b and its application
  • A kind of Lactobacillus brevis 8-2b and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Isolation and identification of Lactobacillus brevis 8-2B

[0031] Bacteria were isolated from Bolongbao winery in Beijing, and the geographical area is 39.6° north latitude and 115.9° east longitude. Wine grape variety: Cabernet Sauvignon. In the ripening season of grapes, use 70% ethanol sterilized plastic bags to cover the picking baskets, and planting rows far away from both sides of the vineyard. Pick an odd row of grapes, select grapes with good growth potential and no disease, and quickly transport them to the laboratory for follow-up after picking. experiment. Cut 50 complete grapes with scissors at different positions on each bunch and place them in a 250mL sterile conical flask, add 100mL sterile water, shake at 180rpm for 1h to obtain a bacterial suspension, and dilute the bacterial suspension to 10 -5 cfu / mL, pipette 100uL of the diluent and spread it on a PDA plate, incubate at 28°C for 24h. The colonies with different shapes or colors on each ...

Embodiment 2

[0035] Example 2 The effect of Lactobacillus brevis 8-2B on the growth and toxigenicity of Aspergillus charcoal

[0036] The concentration of Lactobacillus brevis 8-2B obtained by diluting Example 1 screening to 10 4 , 10 5 , 10 6 , 10 7 cfu / mL, 100 μL was spread to PDA medium, and the control group (CK) was 100 μL of sterile water. take 10 7 5 μL of Aspergillus spore solution of cfu / mL was inoculated into the center of the PDA solid medium inoculated with Brevibacterium, and cultivated in the dark at 28 °C. The diameter of Aspergillus spp. was measured by the cross method every day to calculate the bacteriostatic rate. , 10 mL of 100% methanol was added to each petri dish to extract the OTA toxin, and the OTA production of Aspergillus nigricans was detected by HPLC. Lactobacillus brevis 8-2B inhibits the growth and toxin production of Aspergillus nigricans, see figure 2 ,Table 1. Each set of values ​​represents the mean of 3 replicates.

[0037] like figure 2 Shown (...

Embodiment 3

[0040] Example 3 The effect of Lactobacillus brevis 8-2B on spore germination and germ tube length of Aspergillus nigricans

[0041] The Lactobacillus brevis 8-2B screened in Example 1 was cultured in MRS liquid medium at 28°C for 48h, centrifuged to remove the supernatant, and the precipitate was washed twice with sterile PBS and then prepared into 10 4 , 10 5 , 10 6 and 10 7 cells / mL bacterial suspension for use. Aspergillus niger was inoculated on PDA medium and cultured in a constant temperature incubator at 28°C for 7 days. Rinse the mycelium on the surface of the petri dish with sterile water (containing 0.1% Tween80), then filter it with sterile gauze, use hemocytometry, and adjust the spore concentration to 10 7 cfu / mL. Take 1 mL of different concentrations of bacterial suspension (10 4 , 10 5 , 10 6 and 10 7 cfu / mL) was added to 20 mL of MRS liquid medium, and 1 mL of 10 7 cfu / mL of Aspergillus nigricans spore suspension. 28 ℃, 180rmp culture for 12h. A dr...

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Abstract

The invention relates to the field of microbiology, and specifically discloses a Lactobacillus brevis 8-2B and an application thereof. Lactobacillus brevis 8-2B of the present invention has a deposit number of CGMCC No.20330. The Lactobacillus brevis 8-2B of the present invention can efficiently inhibit the growth and toxin production of Aspergillus nigricans. It has obvious inhibitory effect on the spore germination and germ tube length of Aspergillus carbonifera. It has good development and application prospects in agricultural product preservation, microbial preparations and other antibacterial related fields, and provides an excellent basic strain for the further research and development of microbial-derived OTA inhibitors.

Description

technical field [0001] The invention relates to the field of microbiology, in particular to a Lactobacillus brevis 8-2B and its application. Background technique [0002] Ochratoxin A (OTA) is mainly a toxic secondary metabolite produced by fungi of the genus Aspergillus and Penicillium, and widely contaminates a variety of agricultural products, including grains, grapes, nuts, and spices. . Aspergillus carbonarius is the main source of OTA pollution in agricultural products and is widely distributed. As a natural pollutant, OTA is seriously polluted in all kinds of food and feed. [0003] The toxicity of OTA is second only to aflatoxin, which mainly endangers the kidneys of humans and animals, and is also toxic to the immune system. It also has teratogenic, carcinogenic, and mutagenic effects. At present, the methods for inhibiting the growth of Aspergillus carbonniger and Aspergillus ochra and the formation of OTA can be classified into three types: physical methods, ch...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P3/00A23L3/3571C12R1/24
CPCA01N63/20A23L3/3571A23V2002/00C12R2001/24C12N1/205A23V2400/121A23V2200/10
Inventor 李丽杨博磊刘阳耿海荣王刚张晨曦
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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