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Citrus medica sarcodactylis fermentation extracting solution as well as preparation method and application thereof

A technology of extract and bergamot, applied in the field of bergamot fermentation extract and its preparation, can solve the problems of organic solvent residue, inactivation of active ingredients, increase of production cost, etc., and achieve low production cost, enhanced release, and improved extraction and dissolution Effect

Active Publication Date: 2021-01-22
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The steam distillation method has simple equipment, low cost, and large output, but high-temperature distillation will inactivate some active ingredients; cold pressing is generally processed at an environment below 60°C, and the nutritional components are most completely preserved, but my country's cold pressing technology is not yet Immature and low extraction efficiency; organic solvent extraction is an extraction process carried out with the participation of organic solvents, there will be residual organic solvents in the extract, which is harmful to the human body and pollutes the environment; supercritical CO 2 The extraction method has a good extraction effect, but due to expensive equipment investment, the production cost is greatly increased

Method used

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  • Citrus medica sarcodactylis fermentation extracting solution as well as preparation method and application thereof
  • Citrus medica sarcodactylis fermentation extracting solution as well as preparation method and application thereof
  • Citrus medica sarcodactylis fermentation extracting solution as well as preparation method and application thereof

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preparation example Construction

[0017] The invention provides a method for preparing a bergamot fermentation extract, comprising the following steps: inoculating bergamot freeze-dried powder with fermentation bacteria and performing solid fermentation; performing ultrasonic extraction on the obtained fermented product to obtain a bergamot fermentation extract; the fermentation bacteria include Lactobacillus plantarum and Streptococcus thermophilus.

[0018] In the invention, the bergamot freeze-dried powder is inoculated with fermentation bacteria and then fermented. In the present invention, the particle size of the bergamot freeze-dried powder is preferably 80-300 mesh, more preferably 200 mesh, which can make the fermentation reaction more complete and improve the reaction efficiency. The initial fermentation system of the present invention preferably also includes peptone and potassium dihydrogen phosphate, the quality of the peptone is preferably 0.5% to 5% of the mass of bergamot freeze-dried powder, m...

Embodiment 1

[0027] 5 g of bergamot freeze-dried powder, the amount of peptone added is 1% of the mass of bergamot freeze-dried powder, the amount of potassium dihydrogen phosphate added is 0.15% of the mass of bergamot freeze-dried powder, and the water content of the initial system is 20wt.%. Put it in an autoclave to sterilize, sterilize at 112°C for 15 minutes, take it out, and place it in an ultra-clean workbench to cool for later use. Add live bacteria to 10 7CFU / ml fermented bacteria seed culture solution, the ratio of live bacteria of Lactobacillus plantarum and Streptococcus thermophilus in the fermented bacteria seed culture solution is 1:1, based on the volume of the initial fermentation system, the volume percentage of the inoculum is 45 %, placed in a 40°C incubator and cultivated for 5 days; after the fermentation, the fermented product was ultrasonically extracted with water, and the volume ratio of the fermented product to water was 1g:75ml; the extraction time was 80min, t...

Embodiment 2

[0030] The preparation process is the same as in Example 1, except that: 5 g of bergamot freeze-dried powder in the initial system, the amount of peptone added is 1% of the mass of bergamot freeze-dried powder, and the amount of potassium dihydrogen phosphate added is 0.15% of the mass of bergamot freeze-dried powder ; The water content of the initial system is 60wt.%; 7 CFU / ml, the live bacteria ratio of Lactobacillus plantarum and Streptococcus thermophilus in the fermented bacteria seed culture solution is 9:1; the fermentation time is 5 days, and the fermentation temperature is 30°C; the mass of fermented product and the volume of water in ultrasonic extraction The ratio is 1g:75ml; the extraction time is 80min, the extraction temperature is 45°C, and the extraction power is 300W.

[0031] The appearance of the fermented bergamot extract obtained in this embodiment is light yellow, the pH value is 3.2~4.8, the viscosity is 50~300cP, the mass percentage of soluble solids is...

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Abstract

The invention provides a citrus medica sarcodactylis fermentation extracting solution as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The preparation method of the citrus medica sarcodactylis fermentation extracting solution provided by the invention comprises the following steps of inoculating zymophyte into citrus medica sarcodactylis freeze-dried powder and then carrying out solid fermentation; and carrying out ultrasonic extraction on the obtained fermentation product to obtain the citrus medica sarcodactylis fermentation extractingsolution, wherein the zymophyte comprises lactobacillus plantarum and streptococcus thermophilus. A solid fermentation mode is adopted for citrus medica sarcodactylis, and compared with a common liquid fermentation method, the method has the advantages of being simple in process, environmentally friendly, free of waste liquid discharge, low in cost and the like, and therefore the method has widepracticability and operability. Ultrasonic waves are combined for extraction, so that release, diffusion and permeation of substances in cells are enhanced, and extraction and dissolution of effectivecomponents in plant cells are improved. Water is used as a solvent, so that the extracting solution is harmless to a human body and the environment and is safe and reliable; and raw material sourcesare wide, special equipment is not needed, and production cost is low.

Description

technical field [0001] The invention belongs to the technical field of cosmetics, and in particular relates to a bergamot fermented extract and a preparation method and application thereof. Background technique [0002] The safety of chemically synthesized antioxidants and antibacterial preservatives has been questioned by people, especially in cosmetic products used in the face, people are always worried that they will cause a certain degree of damage to the skin. Bergamot (scientific name: Citrusmedica L.var.sarcodactylis Swingle) belongs to the evergreen small trees or shrubs of the Rutaceae citrus genus. The study found that bergamot essential oil is rich in polyphenols, and contains up to (202.05±9.38) mg / g flavonoids, and flavonoids have a strong scavenging effect on DPPH and OH free radicals, and have anti-hypoxia , anti-rheumatism, analgesia, regulating cardiovascular system, preventing chromosomal aberration, anti-cancer, anti-radiation, anti-inflammation, scavengi...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61Q19/00A61Q19/08
CPCA61K8/9789A61Q19/00A61Q19/08A61K2800/85A61K2800/82Y02A50/30
Inventor 王伟王肖肖刘慧敏刘清雷薛文娟
Owner SHANGHAI INST OF TECH
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