Citrus medica sarcodactylis fermentation extracting solution as well as preparation method and application thereof
A technology of extract and bergamot, applied in the field of bergamot fermentation extract and its preparation, can solve the problems of organic solvent residue, inactivation of active ingredients, increase of production cost, etc., and achieve low production cost, enhanced release, and improved extraction and dissolution Effect
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[0017] The invention provides a method for preparing a bergamot fermentation extract, comprising the following steps: inoculating bergamot freeze-dried powder with fermentation bacteria and performing solid fermentation; performing ultrasonic extraction on the obtained fermented product to obtain a bergamot fermentation extract; the fermentation bacteria include Lactobacillus plantarum and Streptococcus thermophilus.
[0018] In the invention, the bergamot freeze-dried powder is inoculated with fermentation bacteria and then fermented. In the present invention, the particle size of the bergamot freeze-dried powder is preferably 80-300 mesh, more preferably 200 mesh, which can make the fermentation reaction more complete and improve the reaction efficiency. The initial fermentation system of the present invention preferably also includes peptone and potassium dihydrogen phosphate, the quality of the peptone is preferably 0.5% to 5% of the mass of bergamot freeze-dried powder, m...
Embodiment 1
[0027] 5 g of bergamot freeze-dried powder, the amount of peptone added is 1% of the mass of bergamot freeze-dried powder, the amount of potassium dihydrogen phosphate added is 0.15% of the mass of bergamot freeze-dried powder, and the water content of the initial system is 20wt.%. Put it in an autoclave to sterilize, sterilize at 112°C for 15 minutes, take it out, and place it in an ultra-clean workbench to cool for later use. Add live bacteria to 10 7CFU / ml fermented bacteria seed culture solution, the ratio of live bacteria of Lactobacillus plantarum and Streptococcus thermophilus in the fermented bacteria seed culture solution is 1:1, based on the volume of the initial fermentation system, the volume percentage of the inoculum is 45 %, placed in a 40°C incubator and cultivated for 5 days; after the fermentation, the fermented product was ultrasonically extracted with water, and the volume ratio of the fermented product to water was 1g:75ml; the extraction time was 80min, t...
Embodiment 2
[0030] The preparation process is the same as in Example 1, except that: 5 g of bergamot freeze-dried powder in the initial system, the amount of peptone added is 1% of the mass of bergamot freeze-dried powder, and the amount of potassium dihydrogen phosphate added is 0.15% of the mass of bergamot freeze-dried powder ; The water content of the initial system is 60wt.%; 7 CFU / ml, the live bacteria ratio of Lactobacillus plantarum and Streptococcus thermophilus in the fermented bacteria seed culture solution is 9:1; the fermentation time is 5 days, and the fermentation temperature is 30°C; the mass of fermented product and the volume of water in ultrasonic extraction The ratio is 1g:75ml; the extraction time is 80min, the extraction temperature is 45°C, and the extraction power is 300W.
[0031] The appearance of the fermented bergamot extract obtained in this embodiment is light yellow, the pH value is 3.2~4.8, the viscosity is 50~300cP, the mass percentage of soluble solids is...
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Abstract
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