Kit for quantitatively detecting HBP by using magnetic micro-particle chemiluminiscence, preparation method and detection method thereof

Abstract

The invention relates to a kit for quantitatively detecting HBP by using magnetic micro-particle chemiluminiscence, a preparation method and a detection method thereof. The kit comprises an HBP calibrator, an HBP reagent 1, an HBP reagent 2, an HBP magnetic separation reagent and an HBP quality control material, wherein the HBP calibrator comprises six levels, and is composed of HBP antigens withdifferent concentrations and a Tris buffer solution containing bovine serum albumin, the HBP reagent 1 is prepared from biotin ester labeled HBP-Ab and a Tris buffer solution containing bovine serum albumin, the HBP reagent 2 comprises an alkaline phosphatase labeled HBP-Ab and a Tris buffer solution containing bovine serum albumin, the HBP magnetic separation reagent comprises streptavidin labeled magnetic micro particles and a Tris buffer solution containing bovine serum albumin, and the HBP quality control material comprises two quality control levels, and is composed of HBP antigens with different concentrations and a Tris buffer solution containing bovine serum albumin. The kit can be prepared at a low cost, and can realize accurate and high-precision quantitative determination of human heparin binding protein (HBP).

Description

technical field [0001] The invention relates to the technical field of immunological detection, in particular to a kit for quantitatively detecting human heparin-binding protein (HBP) using magnetic particle chemiluminescence, and a preparation method and detection method thereof. Background technique [0002] Human heparin-binding protein (HBP): also known as azurocidin or CAP37, is a granule protein derived from neutrophils, which was first isolated and identified by Shafer et al. in 1984. The structure of HBP consists of a single-chain protein of 222 amino acids, containing 8 cysteine ​​residues, with a glycosylation site on the 100th, 114th or 145th aspartic acid residue, similar in structure to neutrophils Cell elastin has 45% homology with it and 30%-37% homology with other granule-derived serine proteases. Approximately 74% of HBP is stored in azurophilic granules, 18% in secretory vesicles, and 8% on the plasma membrane. [0003] Release mechanism: HBP has been syn...

AI Technical Summary

Problems solved by technology

Immunofluorescence method uses fluorescent antibody to trace or check the corresponding antigen. Its characteristics are: strong specificity, high sensitivity, and fast speed; the main disadvantages are: the problem of non-specific staining has not been completely solved, and the objectivity of the result judgment is insufficient. , the technical procedures are still relatively complicated

Disadvantages: low sensitivity, poor analytical precision

[0009] At present, the application of magnetic particle chemiluminescence assay in human heparin-binding protein (HBP) immunoassay products has not yet been seen.

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