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Edible fungus cultivation material additive and application thereof

A technology for cultivation materials and edible fungi, which is applied in the field of additives for cultivation materials of edible fungi, which can solve the problems of serious diseases and low yield, achieve the effects of promoting growth, improving yield and quality, and inhibiting the growth of harmful fungi

Active Publication Date: 2021-02-02
BIOLOGY INST OF HEBEI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of severe disease and low yield in the existing cultivation process of Agaricus bisporus, the present invention provides an edible fungus cultivation material additive and its application

Method used

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  • Edible fungus cultivation material additive and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Preparation of liquid strains of beneficial microorganisms

[0042] ①Preparation of liquid strains of Lactobacillus plantarum

[0043] Inoculate the activated Lactobacillus plantarum into the MRS medium, and culture it statically at 37°C for 3 days to obtain the liquid strain of Lactobacillus plantarum; the Latin name of the Lactobacillus plantarum is Lactobacillus plantarum, and the depository unit is China Microorganism Culture Collection Committee Ordinary Microbiology Center (abbreviated as CGMCC), the inventor purchased the strain through the strain collection center.

[0044] ②Preparation of liquid strains of Rhodopseudomonas palustris

[0045] Inoculate the activated Rhodopseudomonas palustris into the medium of Rhodopseudomonas paluscans, and culture it under light at 30°C for 5 days to obtain the liquid strain of Rhodopseudomonas palustris, wherein 1L of the medium of Rhodopseudomonas paluscans Including: 1g of ammonium chloride, 1g of sodium bicarbonate,...

Embodiment 2

[0063] (1) With embodiment 1.

[0064] (2) Preparation of biocontrol microbial liquid strains

[0065] ① Preparation of Bacillus amyloliquefaciens BA-KA4 liquid strain

[0066] Inoculate the activated Bacillus amyloliquefaciens BA-KA4 into LB medium, and shake culture at 32°C for 3 days to obtain the liquid strain of Bacillus amyloliquefaciens BA-KA4; the strain preservation number of the Bacillus amyloliquefaciens BA-KA4 It is CGMCC No.12190, the Latin name is Bacillus amyloliquefaciens, and it is the strain disclosed in the patent CN106479934A, the patent name is "Antagonistic strain of Botrytis cinerea and its screening method and application", and it is preserved in China Microorganism Culture Collection Management Committee General Microbiology center.

[0067] ②Preparation of liquid strains of Streptomyces microgreens

[0068] Inoculate the activated Streptomyces chrysanthemum into LB medium, shake culture at 30°C for 3 days to obtain a liquid strain of Streptomyces c...

Embodiment 3

[0081] (1) and (2) are the same as embodiment 1.

[0082] (3) Preparation of nutrient embeddings

[0083] After the cottonseed hulls are crushed into 1-2mm particles, first add 5% (w / v) lime water with the same mass as the cottonseed hulls and stir evenly, then add 50% (w / v) wood pulp with the same mass as the cottonseed hulls and stir Evenly, dry with a hot air dryer to obtain nutrient embeddings.

[0084] (4) Preparation of Edible Fungi Cultivation Material Additive

[0085] 1. Five kinds of beneficial microorganism liquid strains obtained in ①-5. above (1) are mixed in equal volumes and then inoculated into solid fermentation medium A with an inoculum size of 5% (dry components in solid fermentation medium A are measured by mass). Proportioning comprises: 40% of chicken manure, 25% of straw, 20% of corn cob, 10% of bran, 5% of soybean powder and 1% of lime; the mass content of water in the solid fermentation medium is 65%), heap Make piles with a width of 1.5 meters, a h...

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Abstract

The invention relates to the technical field of edible fungus cultivation, and particularly discloses an edible fungus cultivation material additive and application thereof. The edible fungus cultivation material additive comprises a biocontrol microbial fermentation material, a beneficial microbial fermentation material and a nutrient embedding material; the biocontrol microbial fermentation material and the beneficial microbial fermentation material are obtained by fermenting and converting a solid fermentation culture medium through biocontrol microorganisms and beneficial microorganisms respectively; and the edible fungus cultivation material additive is obtained by mixing the biocontrol microbial fermentation material, the beneficial microbial fermentation material and the nutrient embedding material, wherein the dry matter mass content of reducing sugar is less than or equal to 0.3%. The edible fungus cultivation material additive provided by the invention is added into edible fungus cultivation materials such as agaricus bisporus, agaricus subrufescens and straw mushrooms, so that the growth of harmful fungi can be inhibited, the mycelial growth of edible fungi is promoted,slow-release nutrition is provided, the quality of the edible fungi is improved, and the yield and the biological efficiency of the edible fungi are remarkably improved.

Description

technical field [0001] The invention relates to the technical field of edible fungus cultivation, in particular to an edible fungus cultivation material additive and its application. Background technique [0002] Agaricus bisporus is a fungus of the family Agaricaceae of the Basidiomycetes Agaricaceae. It has been cultivated as an edible fungus for more than 300 years and is the world's largest commercially cultivated mushroom species. The traditional cultivation of Agaricus bisporus mainly uses fermentation of rice straw, wheat straw, cow dung, chicken manure, etc. It is an important edible fungus to realize the resource utilization of agricultural waste. The cultivation of Agaricus bisporus in my country also has a history of several decades, but the cultivation volume is still far lower than that of traditional oyster mushrooms, shiitake mushrooms and other edible fungi. In addition to the climate, the reasons for the low output of Agaricus bisporus in my country are als...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/20A01G18/00C12N1/20C12N1/16C12R1/07C12R1/25C12R1/125C12R1/74C12R1/465C12R1/01
CPCA01G18/20A01G18/00C12N1/20C12N1/16
Inventor 张根伟刘振国李书生刘昆昂张玲珲马宏尹淑丽
Owner BIOLOGY INST OF HEBEI ACAD OF SCI
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