Schizophyllan liposome as well as preparation method and application thereof

A technology of Schizophyllum polysaccharide and liposome, which can be used in pharmaceutical formulations, cosmetic preparations, cosmetic preparations, etc., can solve the problems of limited retention, moisturizing or anti-aging effects, and single function, and achieve whitening and anti-aging. Good, uniform and stable particle size distribution, uniform particle size distribution effect

Active Publication Date: 2021-02-05
GUANGDONG MARUBI BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, CN107595724A discloses a skin care base, which includes the following components: deionized water, modified schizophyllan, 1,3-butanediol, phenoxyethanol, Pulmora antarctica extract and chicory root extract, the The skin care base can alleviate the technical problem of the single function of the skin care products in the prior art, and has the functions of anti-aging, moisturizing and repairing at the same time
[0004] However, as a high-molecular and water-soluble natural product, schizophyllan cannot penetrate and stay in the skin well when it is added to cosmetics in a free state, so its moisturizing or anti-aging effects are limited.

Method used

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  • Schizophyllan liposome as well as preparation method and application thereof
  • Schizophyllan liposome as well as preparation method and application thereof
  • Schizophyllan liposome as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] The present embodiment provides a kind of schizophyllan liposome, and its preparation method is as follows:

[0045] (1) Lecithin and cholesterol are dissolved in a mixed solvent of ether / methanol with a volume ratio of 2:1 at a mass ratio of 1:0.25, and the organic solvent is removed by rotary evaporation in a constant temperature water bath at 40°C under reduced pressure to form a uniform lipid film;

[0046] (2) Combine the lipid film obtained in step (1) with the schizophyllan stock solution, surfactant (polysorbate-80 and polyglycerol-10 myristate in a mass ratio of 4:1), pH =7.2 The phosphate buffer is mixed, hydrated until the lipid film is detached to form a milky white liquid, and the crude suspension of schizophyllan liposomes is obtained; wherein the mass ratio of schizophyllan stock solution to lecithin is 0.2:1; The mass ratio of the schizophyllan stock solution to the surfactant is 6:1; the mass ratio of the schizophyllan stock solution to the buffer solut...

Embodiment 2

[0049] The present embodiment provides a kind of schizophyllan liposome, and its preparation method is as follows:

[0050] (1) dissolving lecithin and cholesterol in dichloromethane with a mass ratio of 1:0.1, and removing the organic solvent by rotary evaporation in a constant temperature water bath under reduced pressure at 40°C to form a uniform lipid film;

[0051] (2) The lipid film obtained in step (1) is mixed with the stock solution of schizophyllan, a surfactant (a combination of polysorbate-80 and polyglycerol-10 myristate in a mass ratio of 5:1), pH =7.2 The phosphate buffer solution is mixed, hydrated until the lipid film is detached to form a milky white liquid, and the crude suspension of schizophyllan liposomes is obtained; wherein the mass ratio of schizophyllan stock solution to lecithin is 0.05:1; The mass ratio of the schizophyllan stock solution to the surfactant is 10:1; the mass ratio of the schizophyllan stock solution to the buffer solution is 1:10;

...

Embodiment 3

[0054] The present embodiment provides a kind of schizophyllan liposome, and its preparation method is as follows:

[0055] (1) dissolving lecithin and cholesterol in a mixed solvent of ether / ethanol with a volume ratio of 1:1 at a mass ratio of 1:0.35, and removing the organic solvent by rotary evaporation in a constant temperature water bath at 40° C. to form a uniform lipid film;

[0056] (2) Combine the lipid film obtained in step (1) with the stock solution of schizophyllan, surfactant (polysorbate-80 and glycerol stearate in a mass ratio of 3:1), pH=7.2 Phosphate buffer is mixed and hydrated until the lipid film is detached to form a milky white liquid to obtain a crude suspension of schizophyllan liposomes; wherein the mass ratio of schizophyllan stock solution to lecithin is 0.25:1; schizophyllan stock solution The mass ratio of the surfactant to the surfactant is 5:1; the mass ratio of the schizophyllan stock solution to the buffer solution is 1:20;

[0057] (3) The ...

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Abstract

The invention relates to a schizophyllan liposome as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) dissolving a lipid substance in anorganic solvent, and removing the organic solvent to form a lipid membrane; (2) mixing the lipid membrane with schizophyllan, a surfactant and a buffer solution, and performing hydration until the lipid membrane is separated to form a milk white liquid, so as to obtain a crude suspension of schizophyllan lipidosome; and (3) carrying out ultrasonic treatment on the crude suspension of the schizophyllan liposome, and performing filtering with a filter membrane to obtain the schizophyllan liposome. The schizophyllan serving as a macromolecular and water-soluble natural product is added into cosmetics and cannot well permeate into and stay in the skin in a free state, so that the moisturizing or anti-aging effect of the schizophyllan is limited. The schizophyllan lipidosome is wrapped in lipidosome to prepare a the schizophyllan lipidosome product which is more beneficial to penetration and absorption by skin and retention in the skin, so that the utilization rate of the schizophyllan lipidosome product by skin is remarkably improved, and better moisturizing and anti-aging effects are achieved.

Description

technical field [0001] The invention belongs to the technical field of cosmetics, and relates to a schizophyllan liposome and its preparation method and application, in particular to a schizophyllan liposome with small particle size, uniform distribution and high encapsulation efficiency and its preparation methods and applications. Background technique [0002] Schizophyllan is a water-soluble polysaccharide extracted from the fruiting body, mycelium or fermentation broth of Schizophyllum fungus. It has various physiological activities such as inhibiting tumors, antibacterial and anti-inflammatory, anti-radiation, and improving body immunity. The research on schizophyllan mainly focuses on the biological characteristics, edible and medicinal value, cultivation domestication and submerged fermentation of schizophyllum. [0003] It also has moisturizing and anti-aging effects, and can be added into cosmetics as an active ingredient. There are reports about its application in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/73A61K8/14A61K8/55A61K8/63A61Q19/00A61Q19/02A61Q19/08
CPCA61K8/73A61K8/14A61K8/553A61K8/63A61Q19/00A61Q19/02A61Q19/08A61K2800/56Y02A50/30
Inventor 胡露裴运林聂艳峰郭朝万蒲艳王娟王丽华
Owner GUANGDONG MARUBI BIOLOGICAL TECH CO LTD
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