Edible antioxidant and preparation method and application thereof
A technology of antioxidant and liquefaction treatment, which is applied in the fields of food ingredients as antioxidants, application, and preservation of food ingredients as antimicrobials, etc. The effect of using resources
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[0024] The embodiment of the present invention provides a kind of preparation method of edible antioxidant, it comprises the following steps:
[0025] S1. Mixing the grain powder with water to obtain a slurry;
[0026] S2. The slurry is subjected to protease enzymolysis treatment, amylase liquefaction treatment and glucoamylase enzymolysis treatment to obtain an enzymatic hydrolysis solution;
[0027] S3, inoculating Propionibacterium in the enzymolysis solution for cultivation and fermentation to obtain a fermentation solution;
[0028] S4, removing propionate in the fermented liquid to obtain edible antioxidants.
[0029] The preparation method of the edible antioxidant provided by the embodiment of the present invention has the following advantages:
[0030] First of all, the method uses natural grain powder as the fermentation raw material, which contains various elements and compounds required for the growth and metabolism of Propionibacterium, and does not require addi...
Embodiment 1
[0052] A preparation method of edible antioxidant, comprising the steps of:
[0053] (11) flour and water are mixed according to the mass ratio of 12.7:100 to obtain slurry;
[0054] (12) Adjust the pH value of the slurry to 11.0, insert alkaline protease, control the temperature at 50° C., and stop the reaction when the pH drops below 7.0 and within half an hour to no longer drop; adjust the pH of the slurry to 6.0, add high-temperature amylase, rapidly raise the temperature of the slurry to 90°C and liquefy for 20 minutes, test the slurry with dilute iodine solution until the blue color is no longer visible; adjust the pH of the slurry to 4.5, add 200 U / g of glucoamylase, and react After 15 hours, the reaction ends when the reducing sugar reaches 80% of the total sugar content; the obtained slurry is added to a 2-ton fermenter, and autoclaved at 121°C for 30 minutes;
[0055] (13) Propionibacterium acidipropionici is stored in a glycerol tube and stored in a -80°C refrigerato...
Embodiment 2
[0061] A preparation method of edible antioxidant, comprising the steps of:
[0062] (21) The mixture of rice flour, corn flour and tapioca flour is mixed with water according to the mass ratio of 6:100, while adding 0.2% yeast extract accounting for the total mass of the mixture of rice flour, corn flour and cassava flour, after mixing , get slurry;
[0063] (22) Adjust the pH value of the slurry to 9.0, insert neutral protease, control the temperature at 50°C, and stop the reaction when the pH drops below 6.0 and the pH does not drop within half an hour; adjust the pH of the slurry to 6.0, add high-temperature amylase, rapidly raise the temperature of the slurry to 90°C for liquefaction for 20 minutes, test the slurry with dilute iodine solution until the blue color is no longer visible; adjust the pH of the slurry to 4.5, add 100 U / g of glucoamylase, and react 24 hours, when the reducing sugar reaches 80% of the total sugar content, the reaction ends; the obtained slurry i...
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