Urechis unicinctus polypeptide with antioxidant activity and application thereof
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A technology of anti-oxidative activity and single-ring thorn, which is applied in the field of biomedicine to achieve the effect of reducing the release of LDH, reducing the increase of ROS, and ensuring the integrity
Inactive Publication Date: 2021-03-12
ZHEJIANG OCEAN UNIV
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However, at present, there is no record or report in the prior art about the preparation of antioxidants by polypeptides derived from E.
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Embodiment 1
[0042] Example 1 Effect of the monocyclic echinococcoid polypeptide of the present invention on blood pressure regulatory factors in vascular endothelial cells
[0046] Human umbilical vein endothelial cells (HUVEC) were provided by the Cell Bank of Shanghai Institute of Biological Sciences, Chinese Academy of Sciences.
[0047] 1.2 Main reagents see Table 1
[0048] Table 1 Main Reagents
[0049]
[0050] 1.3 Experimental equipment see Table 2
[0051] Table 2 Experimental Instruments
[0052]
[0053] 2 Experimental methods
[0054] 2.1 Peptide inhibition mode
[0055] According to the methods reported in previous studies (see [Forghani B, Zarei M, Ebrahimpour A, et al. Purification and characterization of angiotensin converting enzyme-inhibitory peptides derived from Stichopus horrens: Stability study against the ACE ...
Embodiment 2
[0119] Example 2 The repairing effect of the monocyclic spinypin polypeptide of the present invention on HUVEC cell damage caused by oxidative stress
[0120] The experimental materials, reagents and instruments used in this example are the same as those in Example 1.
[0121] 1. Experimental method
[0122] 1.1 Determination of superoxide anion scavenging ability
[0123] Prepare F4 and F5 into a series of concentration gradients, add reagents according to Table 6, bathe in water at 25°C for 5 minutes, and measure absorbance at 560nm.
[0124] Table 6 Determination of superoxide anion scavenging rate
[0125]
[0126] The formula for determining the clearance rate is as follows:
[0127] 1.2 Construction of oxidative damage model
[0128] Human umbilical vein endothelial cells (HUVEC) in the logarithmic growth phase were seeded in 96-well culture plates. 180 μL per well, cell number 1.5×10 4 pcs / well, placed at 37°C, 5% CO 2 Incubate for 24 h in an incubator. Div...
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Abstract
The invention provides urechis unicinctus polypeptide with antioxidant activity and application thereof. The urechis unicinctus polypeptide comprises a first polypeptide and / or a second polypeptide, wherein the amino acid sequence of the first polypeptide is as shown in SEQ ID NO. 1; and the amino acid sequence of the second polypeptide is as shown in SEQ ID NO. 2. The urechis unicinctus polypeptide can remove a large number of super-oxide anions generated when Ang II is combined with a receptor of Ang II and reduce the damage degree of endothelial cells, so that hypertension is reduced. Moreover, the urechis unicinctus polypeptide has a remarkable protection effect on HUVEC cells damaged by H2O2 and can promote release of a vasodilatation factor NO, so that endothelial cells are protectedto a certain extent, the SOD content and MDA content in oxidative damage cells can be increased, the CAT activity in the oxidative damage cells is improved, the release of LDH in oxidative damage cells is reduced, and the rise of ROS in the cells caused by oxidative damage is reduced, so that the integrity of cell membranes is ensured, and the damage to the cells is reduced.
Description
technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a monocyclic echinacea polypeptide with antioxidant activity and its application. Background technique [0002] Free radicals refer to a class of substances that are widely distributed in the human body and exist in free form. Free genes carry unpaired electrons and are unstable, and are prone to nucleophilic and electrophilic reactions in the body, causing damage to biological macromolecules (nucleic acid, protein, lipid, etc.) in the body. A class of free radicals containing reduced oxygen produced by normal metabolism in the human body or excessively produced by body damage is collectively called reactive oxygen species (ROS). [0003] ROS play an important signaling role in cells, such as regulating intracellular Ca 2+ Secondly, ROS are active reaction intermediates, which can trigger a series of rapid chain reactions and cause damage to lipids, proteins, ca...
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