Immune quantitative detection device, detection method and application of cardiovascular disease markers

A technology of immune quantification and detection method, which is applied in measuring devices, disease diagnosis, biological testing, etc., and can solve the problems that immune detection technology cannot be realized at the same time

Pending Publication Date: 2021-03-12
绍兴梅奥心磁医疗科技有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] High sensitivity, rapidity, miniaturization, full quantification, and automation are the current development trends of clin...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Immune quantitative detection device, detection method and application of cardiovascular disease markers
  • Immune quantitative detection device, detection method and application of cardiovascular disease markers
  • Immune quantitative detection device, detection method and application of cardiovascular disease markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Embodiment 1: the preparation of filter of the present invention

[0072] Experimental materials: commercially available microfilters, NHS activated agarose gel particles (ACRO BIOSYSTEMS), anti-human fibrinogen polyclonal antibody (Genagates Inc.), sodium bicarbonate, hydrochloric acid, ethanolamine.

[0073] Experimental method: Take a commercially available microfilter and take out the filter element; take 5 mg of anti-human fibrinogen polyclonal antibody plus 0.2M sodium bicarbonate solution, pH 8.3, and dissolve in 2ml; take 1ml of NHS-activated agarose gel particles, and use 20ml of 1mM hydrochloric acid, wash by suction filtration three times; mix the anti-human fibrinogen polyclonal antibody solution with the treated NHS-activated agarose gel particles, and shake for 4 hours at 4°C; the NHS-activated agarose gel after washing reaction gel particles, then add 10mM ethanolamine, 0.2M sodium carbonate solution, pH8.0, shake at room temperature for 4 hours to block ...

Embodiment 2

[0074] Embodiment 2: Comparison of detection performance between the present invention and existing chemiluminescence detection technology

[0075] Experimental materials: anti-human fibrinogen polyclonal antibody filter, horseradish peroxidase-labeled anti-human fibrinogen monoclonal antibody, magnetic particles, luminol, p-iodophenol, carbamide peroxide, chemiluminescence detector, human Fibrinogen solution.

[0076] Experimental method: Take human fibrinogen solution of known concentration, dilute it with PBS solution to prepare 1ug / ml human fibrinogen solution. The experimental method will use the present invention and the current chemiluminescence detection technology to observe the influence of different binding reaction times on the amount of luminescence. Observe the binding reaction time points 1, 2, 4, 10, 20, 30, 45, 60 minutes.

[0077] For the current chemiluminescence detection technology group, add 100ul of magnetic particles labeled with anti-human fibrinogen...

Embodiment 3

[0080] Embodiment 3: Comparison of the present invention with the detection results of the current chemiluminescence detection technology

[0081] Experimental materials: anti-human fibrinogen polyclonal antibody filter, horseradish peroxidase-labeled anti-human fibrinogen monoclonal antibody, magnetic particles, luminol, p-iodophenol, carbamide peroxide, chemiluminescence detector, human Fibrinogen solution, healthy human plasma.

[0082] Experimental method: take a human fibrinogen standard solution of known concentration, dilute it with PBS solution to prepare 10, 30, 70, 100, 300, 700ng / ml human fibrinogen solution; take another healthy person's plasma, and use PBS for 10000 times Dilution; experimental method adopts the present invention and existing chemiluminescence detection technology to detect human fibrinogen solution and draws standard curve, then measures healthy human fibrinogen, and calculates fibrinogen concentration with standard curve; Get 42 test tubes, be d...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides an immune quantitative detection device for cardiovascular disease markers, which is characterized by comprising a mobile phase containing device (2), a filter (1), a detectionphase containing device (3) and a detector (4), wherein the mobile phase containing device (2) is connected with the filter (1) through a pipeline (8); the filter (1) comprises an inlet (5), a filterelement (6) and an outlet (7); the detection phase containing device (3) is connected with the detector (4) through a pipeline (9); and the filter element (6) is made of a substance which can be coupled by using a cardiovascular disease marker specific conjugate. The invention further provides an immune quantitative detection method and an immune quantitative detection amplification method of thecardiovascular disease marker and related application. According to the invention, the individual controllable degree of immune quantitative detection is improved, and the detection efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of immunoquantitative detection, in particular to an immunoquantitative detection device for cardiovascular disease markers, an immunoquantitative detection method, an immunoquantitative amplification detection method and its application. Background technique [0002] Immunological detection technology is an experimental method designed by applying the principles of immunology to determine antigens, antibodies, immune cells and chemical components. Samples for analytical, food and industrial analysis. Commonly used immunological detection techniques include immunoturbidimetric technology, solid-phase enzyme immunoassay technology, chemiluminescent detection technology, immunofluorescence labeling technology, flow cytometry, colloidal gold technology, etc. [0003] Among them, the immune turbidity technique, also known as the immune turbidity method, refers to the specific binding of soluble antigens and ant...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/68G01N33/573
CPCG01N33/6893G01N33/573G01N2333/4712G01N2333/9123G01N2800/32
Inventor 刘凤鸣张玉霄陈越猛张新龙张煊浩
Owner 绍兴梅奥心磁医疗科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap