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A synbiotic and feed for improving the intestinal health and immunity of turbot

A technology of turbot and synbiotics, applied to animal feed, animal feed, bacteria used in food preparation, etc., can solve problems such as failure to achieve expected results, lack of scientific basis for compatibility, waste of resources, etc., to improve fish body Level of immune response, promotion of gut health, effects of quality assurance

Active Publication Date: 2022-05-13
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For biostime products, many product combinations do not have a scientific basis, not only failing to achieve the desired effect, but also causing a waste of resources

Method used

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  • A synbiotic and feed for improving the intestinal health and immunity of turbot
  • A synbiotic and feed for improving the intestinal health and immunity of turbot
  • A synbiotic and feed for improving the intestinal health and immunity of turbot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] First, the protective effect of stachyose on the intestinal health of turbot fed with high levels of cottonseed meal was evaluated through breeding experiments, and the appropriate dosage of stachyose in the feed was determined, combined with high-throughput sequencing analysis of intestinal flora As a result, the probiotic strains suitable for collocation with stachyose were screened out.

[0028] The specific operation method is as follows: prepare 4 kinds of feeds with equal nitrogen and equal fat, the content of crude protein is 52%, and the content of crude fat is 12%. In the fishmeal group, fishmeal was used as the main protein source, and cottonseed meal was used to replace 40% of the fishmeal protein to make the cottonmeal group, and stachyose was added to the feed base of the cottonmeal group to make 1.4% stachyose group and 2.8% stachyose group Group. The stachyose (purity ≥ 80.0%) used in this experiment was purchased from China Institute of Food and Ferment...

Embodiment 2

[0059] The preparation of embodiment 2 turbot synbiotics:

[0060] After mixing 2.5% stachyose with other raw materials, it is extruded and dried to make hard pellet feed. Cultivate the bacteria in MRS medium overnight, take 1ml of the bacteria solution and centrifuge at 3500g for 5min, resuspend twice in PBS, calculate the OD value with a microplate reader, and calculate the concentration of the bacteria according to the pre-drawn bacteria number-OD value curve , according to 1×10 8 CFU / g concentration, spray it into the feed containing stachyose and mix thoroughly.

[0061] The stachyose was purchased from China National Research Institute of Food and Fermentation industries Co., Ltd, and was extracted from Caddis worm with a purity of ≥80%.

[0062] The Lactobacillus casei was purchased from China Center of Industrial Culture Collection (CICC for short), and the strain preservation number is CICC 6117.

Embodiment 3

[0063] Example 3 The application of synbiotics to improve the immune response ability and intestinal health of turbot

[0064] 2.5% stachyose, 1×10 8 CFU / g Lactobacillus casei and at the same time add 2.5% stachyose and 1×10 8 CFU / g Lactobacillus casei (synbiotic group), the control group is the basal feed without adding stachyose and Lactobacillus casei, and is made into 4 groups of feeds with equal nitrogen and energy, in which stachyose and the basal feed of the control group are added The formula is shown in Table 5, and Lactobacillus casei is added in the basal feed and the stachyose-containing feed respectively (two groups are not listed). Feed raw materials are ultrafinely pulverized and passed through a 60-mesh sieve, and the raw materials are mixed evenly according to the proportion and quantified. The feed with a particle size of 3mm is extruded by a twin-screw extruder, dried in a ventilated oven at 55°C for about 12 hours, and then stored in Store in -20°C refrig...

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Abstract

The invention relates to a synbiotic and feed for improving the intestinal health and body immunity of turbot, belonging to the technical field of aquaculture feed probiotic additives. The synbiotic is composed of stachyose and Lactobacillus casei. The present invention also provides a turbot feed containing the synbiotic, wherein the mass ratio of stachyose in the synbiotic to the turbot feed is 2.0-3.0%, and the Lactobacillus casei in the synbiotic is in the turbot feed. The concentration in flounder feed is 1×10 7 ‑1×10 9 CFU / g. The feed can significantly increase the abundance of a specific probiotic strain Lactobacillus casei in the intestine, improve the intestinal morphology and structural integrity, and improve the immune response ability of turbot, thereby improving the intestinal health and disease resistance of cultured turbot. .

Description

technical field [0001] The invention belongs to the technical field of aquaculture feed additives, and in particular relates to a synbiotic additive and feed for promoting intestinal health and immune response level of turbot. The synbiotic is formed by combining stachyose and Lactobacillus casei . Background technique [0002] In recent years, the contribution of aquaculture to world food production has increased significantly, and the advancement of farming technology and the introduction of new species have stimulated the rapid development of aquaculture. However, due to the limitation of aquaculture resources such as water environment and land, high-density aquaculture is still an inevitable trend. In addition, due to the extreme shortage of fishmeal resources, plant protein sources with stable sources and low prices are widely used in aquafeeds, and their application is usually easy to cause enteritis in farmed fish, especially carnivorous farmed fish. Intestinal trac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K20/163A23K10/18A23K50/80
CPCA23K20/163A23K10/18A23K50/80A23V2400/169Y02A40/818
Inventor 张彦娇戴济鸿张文兵艾庆辉麦康森
Owner OCEAN UNIV OF CHINA
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