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Method for producing polymer used for cell culture base film, and cell culture vessel

A manufacturing method and cell culture technology, which are applied in the methods of supporting/immobilizing microorganisms, culturing devices for tissue cells/viruses, biochemical equipment and methods, etc., can solve the problem of difficult to control the ratio of hydrolysis, difficult to control molecular weight, molecular weight distribution, Difficulty in controlling molecular weight, etc.

Pending Publication Date: 2021-03-26
NISSAN CHEM IND LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in the case of producing a PDMAEMA-derived polymer having a cationic 2-N,N-dimethylaminoethyl group and an anionic carboxyl group using the above-mentioned conventional PDMAEMA production method, there is the following problem: DMAEMA whose side chain has a cationic 2-N,N-dimethylaminoethyl group through an ester group is irradiated with ultraviolet rays to undergo radical polymerization, and an anionic DMAEMA needs to be generated by hydrolyzing a part of the ester group. Carboxyl group requires more steps for production, and it is difficult to control the rate of hydrolysis, and it is difficult to control the molecular weight
[0009] However, in the case of using the above-mentioned production method, it is still difficult to control the ratio of the cationic functional group to the anionic functional group, and it is difficult to control the molecular weight and molecular weight distribution.

Method used

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  • Method for producing polymer used for cell culture base film, and cell culture vessel
  • Method for producing polymer used for cell culture base film, and cell culture vessel
  • Method for producing polymer used for cell culture base film, and cell culture vessel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0198] Hereinafter, the present invention will be described in more detail by giving examples, but the present invention is not limited to these examples.

[0199]

[0200] The weight average molecular weights shown in the following synthesis examples are based on the results of gel filtration chromatography (Gel Filtration Chromatography) (hereinafter, abbreviated as GFC).

[0201] (measurement conditions)

[0202] ・Apparatus: HLC-8320GPC (manufactured by Tosoh Co., Ltd.)

[0203] ·GFC column: TSKgel G6000+3000PWXL-CP

[0204] ·Flow rate: 1.0ml / min

[0205] Eluent: water / organic mixed solvent containing salt

[0206] ·Column temperature: 40℃

[0207] Detector: RI

[0208] ・Injection concentration: 0.05% by mass of polymer solid content

[0209] ·Injection volume: 100μL

[0210] Standard curve: cubic fitting curve

[0211] ・Standard sample: polyethylene oxide (manufactured by Agilent) x 10 types

Synthetic example 1

[0212] Production of a polymer used as a basement membrane-forming agent for cell culture by thermal polymerization (1)

[0213] To 9.00 g of 2-(dimethylamino)ethyl methacrylate (manufactured by Tokyo Chemical Industry Co., Ltd.), 27.04 g of tetrahydrofuran was added and fully dissolved. Then, 0.01 g of dimethyl 1,1'-azobis(1-cyclohexanecarboxylate) (VE-073, manufactured by FUJIFILM Wako Pure Chemical Corporation) was added to the tetrahydrofuran solution while keeping the temperature at 20°C or lower. Add the mixed solution containing all the above substances that is fully stirred until uniform to a three-necked flask with a condenser tube, and raise the temperature to the reflux temperature while stirring under nitrogen flow. A polymer was obtained as a reaction product by heating and stirring while maintaining the above-mentioned environment for 24 hours. The reaction product was reprecipitated in hexane as a poor solvent, and the precipitate was collected by filtration a...

Synthetic example 2

[0215] Production of a polymer used as a basement membrane-forming agent for cell culture by thermal polymerization (2)

[0216] To 9.00 g of 2-(dimethylamino)ethyl methacrylate (manufactured by Tokyo Chemical Industry Co., Ltd.), 37.10 g of tetrahydrofuran was added and fully dissolved. Then, 0.02 g of dimethyl 1,1′-azobis(1-cyclohexanecarboxylate) (VE-073, manufactured by FUJIFILM Wako Pure Chemical Corporation) and methyl Acrylic acid (manufactured by Tokyo Chemical Industry Co., Ltd.) 0.26 g. Add the mixed solution containing all the above substances that is fully stirred until uniform to a three-necked flask with a condenser tube, and raise the temperature to the reflux temperature while stirring under nitrogen flow. A polymer was obtained as a reaction product by heating and stirring while maintaining the above-mentioned environment for 24 hours. The reaction product was reprecipitated in hexane as a poor solvent, and the precipitate was collected by filtration and dr...

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Abstract

Provided are: a method for producing a polymer used for a cell culture base film; a cell culture base film-forming agent; a base film; and a method for producing a cell culture vessel. The method forproducing a polymer used for a cell culture base film comprises: a preparation step for preparing a mixture containing a monomer represented by formula (I) [in the formula, Ua1 and Ua2, each independently, represent a hydrogen atom or a C1-5 linear or branched alkyl group, Ra1 represents a hydrogen atom or a C1-5 linear or branched alkyl group, and Ra2 represents a C1-5 linear or branched alkylenegroup], a radical polymerization initiator, and an organic solvent; and a polymerization step for heating and stirring the mixture and polymerizing the monomer to prepare a polymer.

Description

technical field [0001] The present invention relates to a method for producing a polymer used as a basement membrane for cell culture, and to production of a cell culture container having the basement membrane. Background technique [0002] Poly(2-N,N-dimethylaminoethyl methacrylate) (PDMAEMA), a polymer of 2-N,N-dimethylaminoethyl methacrylate (DMAEMA), has a turbidity of about 32°C. Dot temperature-responsive polymers are known. [0003] In the conventional method for producing PDMAEMA, a mixture containing DMAEMA is prepared (preparation step), and the mixture is irradiated with ultraviolet rays (irradiation step) to radically polymerize DMAEMA, thereby producing PDMAEMA. [0004] Here, there is also a case where a part of the ester group containing a cationic 2-N,N-dimethylaminoethyl group bonded to the side chain of PDMAEMA is converted into an anionic carboxyl group by hydrolysis, and the physical properties are changed. Demand for polymers derived from PDMAEMA. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/07C08F2/06C08F220/06C08F220/20C08F220/34C12M1/00C12N1/00C12M3/00
CPCC08F2/06C12M23/20C12M25/06C08F220/36C09D133/14C08F220/34C08F220/06C08F222/20C08F230/02C08F2/44C08J5/18C12M25/02C12N1/00
Inventor 铃木康平广井佳臣安部菜月
Owner NISSAN CHEM IND LTD