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A kind of blood lipid adsorbent and preparation method thereof

An adsorbent and blood lipid technology, applied in the field of blood lipid adsorbent and its preparation, can solve the problems of low reaction efficiency, inability to obtain blood lipid adsorbent, low utilization rate of ligands, etc., so as to improve adsorption efficiency, improve catalytic ring opening and coupling efficiency. The effect of improving the efficiency of coupling reaction and coupling reaction

Active Publication Date: 2022-06-10
WUHAN RUIFA MEDICAL DEVICES CO LTD
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Problems solved by technology

However, in the actual coupling reaction, the utilization rate of the ligand dextran sulfate is less than 1%, and the effective blood lipid adsorbent cannot be obtained when the reacted ligand solution is reused. It can be seen that the reaction efficiency of the coupling reaction Low and low ligand utilization
[0005] In view of this, it is necessary to design an improved method for the preparation of lipid adsorbents to solve the problems of low reaction efficiency and low utilization of ligands during the preparation of adsorbents.

Method used

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  • A kind of blood lipid adsorbent and preparation method thereof
  • A kind of blood lipid adsorbent and preparation method thereof

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preparation example Construction

[0026] The invention provides a preparation method of a blood lipid adsorbent, comprising the following steps:

[0027] S1. Activating the adsorption carrier to obtain an epoxy-containing activation carrier;

[0028] The epoxy-containing activation carrier includes, but is not limited to, one or more of epoxy-containing sepharose, cellulose gel and resin, preferably sepharose microspheres. For example, epichlorohydrin is used to activate the agarose gel microspheres to obtain epoxy group-containing agarose gel microspheres.

[0029] S2. adding the lipid adsorption ligand into the buffer to obtain a ligand solution;

[0030] The blood lipid adsorption ligand is a dextran sulfate solution or a heparin solution; the buffer is a glycine-sodium hydroxide buffer.

[0031] S3. The epoxy-containing activation carrier obtained in step S1 is mixed with a tertiary amine reagent, and then added to the ligand solution obtained in step S2, and the coupling reaction is carried out with mix...

Embodiment 1

[0040] A preparation method of a blood lipid adsorbent, comprising the following steps:

[0041] S1. Take a certain amount of agarose gel microspheres, add 1.0mol / L sodium hydroxide solution and epichlorohydrin in a ratio of 1g:1.5ml:450μl to the microspheres, mix well, and react at 40°C for 2 hours , washed and drained to obtain activated agarose gel microspheres;

[0042] S2. Dissolve dextran sulfate sodium salt in a glycine-sodium hydroxide buffer with a pH value of 10.6 at a ratio of 200mg: 1ml to prepare a dextran sulfate ligand solution;

[0043] S3. Mix the activated sepharose microspheres with dodecyl tertiary amine by 1g:0.6ml, and then press the ratio of activated sepharose microspheres to dextran sulfate ligand solution to be 1g:2.5ml , adding it into the dextran sulfate ligand solution, carrying out a coupling reaction at 50° C. for 8 hours, and then washing the adsorption carrier with purified water, brine, ethanol and purified water in turn to obtain the blood l...

Embodiment 2

[0045] A preparation method of a blood lipid adsorbent, compared with Example 1, is different in that the dodecyl tertiary amine in step S3 is replaced with hexadecyl tertiary amine. Others are basically the same as those in Embodiment 1, and are not repeated here.

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Abstract

The invention provides a blood lipid adsorbent and a preparation method thereof. In this method, the adsorption carrier is firstly activated to obtain an activated carrier containing epoxy groups; then the lipid adsorption ligand is added to glycine-sodium hydroxide buffer to obtain a ligand solution; the obtained epoxy group-containing activated carrier is The carrier is mixed with the tertiary amine reagent, then added to the ligand solution, mixed and stirred to carry out the coupling reaction, after the reaction is completed, the solid-liquid separation, cleaning and drying are carried out to obtain the blood lipid adsorbent. The present invention is by adding tertiary amine reagent in coupling reaction system, and tertiary amine reagent is first adsorbed on the surface of activated carrier containing epoxy group, then coupling reaction in glycine-sodium hydroxide buffer solution ligand solution, can improve The efficiency of the coupling reaction and the utilization rate of the ligand not only improve the adsorption efficiency of the adsorbent, but also reduce the production cost.

Description

technical field [0001] The invention relates to the technical field of preparation of medical materials, in particular to a blood lipid adsorbent and a preparation method thereof. Background technique [0002] Hyperlipidemia is a condition in which one or more lipids in the plasma are higher than normal due to abnormal fat metabolism or operation Lipoprotein is too low, which is called "dyslipidemia" in modern medicine. Hyperlipidemia is a common and frequently-occurring disease, and it is also the culprit of cardiovascular and cerebrovascular diseases. The damage to the body is hidden, gradual, progressive and systemic. Its direct damage is to accelerate systemic atherosclerosis. . Among them, low-density lipoprotein is a lipoprotein particle that carries cholesterol into peripheral tissue cells. When low-density lipoprotein is excessive, the cholesterol it carries will accumulate on the arterial wall, which is easy to cause atherosclerosis for a long time. Therefore, LD...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/24B01J20/30
CPCB01J20/24B01J20/22
Inventor 张媛张磊陈梦林沈乔皮云王业富
Owner WUHAN RUIFA MEDICAL DEVICES CO LTD
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