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A kind of core-shell structure microsphere with oxygen generating effect and preparation method thereof

A core-shell structure and microsphere technology is applied in the field of core-shell structure microspheres with oxygen production and preparation thereof, which can solve the problems of low oxygen release, limited oxygen production, and influence on the efficiency of oxygen production, etc. The effect of slowing down decomposition, avoiding secretion, and ensuring safe use

Active Publication Date: 2022-08-05
WUHAN TEXTILE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method is only applicable to tumor cells that contain a relatively high concentration of hydrogen peroxide. For normal tissues with a very low concentration of hydrogen peroxide, the efficiency of oxygen production will be significantly and directly affected.
Moreover, the oxygen production of self-oxygen nanoparticles prepared in this patent is limited by the hydrogen peroxide content in tumor cells, which leads to the problem of low oxygen release.

Method used

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  • A kind of core-shell structure microsphere with oxygen generating effect and preparation method thereof
  • A kind of core-shell structure microsphere with oxygen generating effect and preparation method thereof
  • A kind of core-shell structure microsphere with oxygen generating effect and preparation method thereof

Examples

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Effect test

preparation example Construction

[0037] The present invention provides a method for preparing core-shell microspheres with oxygen-generating effect. The schematic diagram of the preparation process is as follows: figure 1 shown, including the following steps:

[0038]S1, the polylactic acid-glycolic acid copolymer is dissolved in an organic solvent according to a preset concentration to form an oil phase;

[0039] S2, fully mixing hydrogen peroxide and polyvinylpyrrolidone to form a complex to obtain an aqueous phase;

[0040] S3, slowly adding the water phase obtained in step S2 to the oil phase obtained in step S1, and adding polyvinyl alcohol to obtain a primary emulsion after homogenization;

[0041] S4, adding the primary emulsion obtained in step S3 into the continuous phase containing polyvinyl alcohol and hydrogen peroxide to form a secondary emulsion;

[0042] S5. After fully stirring the secondary emulsion obtained in step S4, the organic solvent in step S1 is removed by evaporation, and then cent...

Embodiment 1

[0051] The present embodiment provides a method for preparing core-shell microspheres with oxygen generation, comprising the following steps:

[0052] S1. Dissolve polylactic acid-glycolic acid copolymer (PLGA) in dichloromethane (DCM) solution, and control the concentration of PLGA to be 12wt% to form an oil phase (O 1 ).

[0053] S2, the hydrogen peroxide (H 2 O 2 ) mixed with polyvinylpyrrolidone (PVP) in a molar ratio of 1:7.5, and stirred overnight at -4°C to form an aqueous phase (W 1 ); where H 2 O 2 Intensive mixing with PVP can form stable complexes.

[0054] S3. 0.3 mL of the water phase obtained in step S2 was added dropwise to 2 mL of the oil phase obtained in step S1, and then 2 mL of polyvinyl alcohol with a concentration of 1% was added as a stabilizer and emulsifier, using a high-speed homogenizer at 10,000 rpm Homogenize for 60 s at a rotating speed of / min to obtain a water-in-oil primary emulsion (W 1 / O 1 ).

[0055] S4, pour the whole primary emul...

Embodiment 2～4

[0059] Embodiments 2 to 4 respectively provide a preparation method of core-shell structure microspheres with oxygen production. Compared with embodiment 1, the difference is that the PVA concentration in the continuous phase in step S4 is changed, and the remaining steps are the same. It is consistent with Embodiment 1 and will not be repeated here. The PVA concentrations corresponding to Examples 2 to 4 were 0.6%, 0.8% and 1.2%, respectively.

[0060] The oxygen production of the core-shell structure microspheres with oxygen production prepared in Example 1 and Examples 2 to 4 is tested, and the specific steps include the following steps:

[0061] According to catalase: deionized water=1:100, a catalase solution with a concentration of 1% was prepared, and then the microspheres prepared in Examples 1 to 4 were put into the catalase solution for 0 min, The amount of oxygen produced was tested at 30min, 60min and 90min.

[0062] According to the above test results, the compa...

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Abstract

The invention provides a core-shell structure microsphere with oxygen generating effect and a preparation method thereof. A water-in-oil primary emulsion is formed by adding the aqueous phase formed by the complexation of hydrogen peroxide and polyvinylpyrrolidone into the oil phase containing polylactic acid-glycolic acid copolymer; In the phase, a water-in-oil-in-water secondary emulsion is formed, and after removing the solvent, centrifugation, washing and drying are performed to prepare core-shell microspheres with hydrogen peroxide as the core body and polylactic acid-glycolic acid copolymer as the shell layer. . Through the above method, the present invention can stably complex polyvinylpyrrolidone and hydrogen peroxide to slow down the decomposition of hydrogen peroxide; and utilize the core-shell structure of the microspheres to avoid direct contact between hydrogen peroxide and cells, while ensuring the safety of cells The hydrogen peroxide reacts under the catalysis of catalase in the body to generate oxygen and water to achieve the effect of oxygen production.

Description

technical field [0001] The invention relates to the technical field of oxygen-generating materials, in particular to a core-shell structure microsphere with oxygen-generating effect and a preparation method thereof. Background technique [0002] When the skin is damaged, the tissue is rapidly deprived of oxygen and ischemia. Under the condition of sufficient oxygen, oxygen will lead to the formation of new blood vessels, while hypoxia will lead to apoptosis and aggravated inflammation. Vicious circle. More importantly, when the damaged tissue senses its own hypoxia-related signals, it will automatically release the "hypoxia-inducible factor", which induces excessive secretion of collagen, excessive deposition and disorder of arrangement, and finally leads to abnormal wound healing. scar. The formation of scars often causes deformities, contractures and loss of motor function of the skin tissue. Long-term chronic scars also have the risk of inducing cancer, which not only ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/50A61K47/34A61K47/58A61K33/40A61P17/02
CPCA61K9/5031A61K47/58A61K33/40A61P17/02
Inventor 张强周水清闫书芹尤仁传李秀芳罗祖维徐安长
Owner WUHAN TEXTILE UNIV
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