Bone defect repair material based on modified perovskite quantum dots and preparation method of bone defect repair material

Abstract

The invention discloses a bone defect repair material based on modified perovskite quantum dots and a preparation method of the bone defect repair material. The bone defect repair material comprises the following raw materials in percentage by mass of 20-30% of hydroxyapatite, 10-20% of octacalcium phosphate, 0.5-2% of bone morphogenetic protein, 1-5% of collagen, 0.5-2% of chondroitin sulfate, 3-5% of carboxymethyl chitosan and the balance of a modified perovskite quantum dot solution. The preparation method comprises the following steps of S101, adding bone morphogenetic protein, collagen and chondroitin sulfate into the modified perovskite quantum dot solution, and stirring to react for 0.5-1 hour to obtain a dispersion solution A; S102, adding hydroxyapatite and octacalcium phosphate into the dispersion solution A, soaking and adsorbing for 3-6 hours, and uniformly stirring to obtain dispersion slurry B; and S103, adding carboxymethyl chitosan into the dispersion slurry B, and stirring at 45-60 DEG C to react for 2-6 hours. Perovskite quantum dots are introduced into the bone defect repair material for the first time, and stem cell behavior changes are monitored through perovskite quantum dot mediated stem cell transfection and fluorescence; and meanwhile, the stem cells are induced to differentiate towards the directions of osteoblasts and chondroblasts.

Description

technical field [0001] The invention belongs to the field of medical materials, in particular to a bone defect repair material based on modified perovskite quantum dots and a preparation method thereof. Background technique [0002] A bone defect is a bone deficiency caused during a pathological process or during surgery, which disrupts the structural integrity of the bone. Clinically, trauma, tumors, osteomyelitis, various congenital diseases, and surgical operations may all cause bone defect symptoms. Due to the existence of bone defects, bone nonunion, delayed union or nonunion, and local dysfunction are often caused. At present, the methods for treating bone defects include autologous bone and allogeneic bone transplantation, tissue engineering technology, gene therapy rejection method, and physical factor therapy. However, autologous bone transplantation will cause secondary trauma, and allogeneic bone transplantation may cause immune rejection. Engineering technology...

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Problems solved by technology

However, due to its strong ionicity, high surface energy and metastable structure, perovskite quantum dots are highly sensitive to the environment, and are easy to cause phase transition, agglomeration or even degradation under the action of polar solvents, water and oxygen, thereby causing fluorescence quenching. off, especially with Sn 2+ As a B-site element, it is easily oxidized to Sn in humid air 4+ , exacerbating the main defects of halogen vacancies and interstitial metals, further accelerating the collapse of the perovskite structure

Due to the poor stability of perovskite quantum dots, its practical application is greatly limited

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