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Composition with barrier repairing and anti-aging effects as well as preparation method and application thereof

A composition and anti-aging technology, applied in the directions of medical preparations containing active ingredients, preparations for skin care, pharmaceutical formulas, etc., can solve the problem of not being able to adjust the mineral elements in the epidermis to enhance the barrier function of the epidermis, etc.

Active Publication Date: 2021-04-30
COSMAX CHINA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the defect that the anti-aging composition in the prior art can only be used for skin repair after aging, but cannot regulate the mineral elements in the epidermis to enhance the barrier function of the epidermis, The present invention provides a composition with the effect of "maintaining internal stability and external repairing", that is, regulating the mineral elements in the epidermis to enhance the barrier function of the epidermis and repairing the skin damage caused by external stimuli, and has the effect of prevention and repair

Method used

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  • Composition with barrier repairing and anti-aging effects as well as preparation method and application thereof
  • Composition with barrier repairing and anti-aging effects as well as preparation method and application thereof
  • Composition with barrier repairing and anti-aging effects as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0049] Cytotoxicity test:

[0050] MTT assay is an experimental method widely used in the detection of cytotoxicity or cell proliferation by measuring the number of living cells. The detection principle is that succinate dehydrogenase in the mitochondria of living cells can make the water-soluble yellow salt MTT (3- (4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide) is reduced to water-insoluble blue formazan and deposited in cells, while dead cells have no such function. The generated crystalline formazan is generally added to DMSO (dimethyl sulfoxide), and the absorbance is measured after dissolution. The specific experimental method is in a 96-well plate according to 1X10 4 Inoculate 100 μl of DMEM medium containing 10% bovine serum and 100 μl of keratinocytes (HaCaT) at a density of one per well, and replace with serum-free medium after 24 hours of culture. Different concentrations of the composition were added to the serum-free medium for treatment and cultured f...

experiment example 2

[0057] Antioxidant evaluation test:

[0058] DPPH, also known as 1,1-diphenyl-2-trinitrophenylhydrazine, is a very stable nitrogen-centered free radical. Its anhydrous ethanol solution is purple. When adding a free radical scavenger, the number of free radicals The absorbance becomes smaller and the color of the solution becomes lighter, which can evaluate the ability to scavenge free radicals.

[0059] Measure 40 μl of each composition with a pipette, then add 160 μl of DPPH ethanol solution (200 μg / ml) to dilute and mix evenly. After mixing, react at room temperature for 30 minutes in the dark, and then measure the absorbance A at 517nm. Three groups of parallel experiments were set up in each group to calculate the average value, and the calculation method was as follows:

[0060] Clearance %=[1-(A 1 -A 2 ) / A 0 ×100

[0061] In the formula: A 0 For not adding the absorbance of the DPPH solution of each composition;

[0062] A 1 is the absorbance after adding each co...

experiment example 3

[0073] Keratinocyte gene expression assay:

[0074] Filaggrin (FLG) is an important condition for the terminal differentiation of keratinocytes and plays an important role in the differentiation and formation of the epidermis. Caspase14 (CASP14, aspartase proteolytic enzyme) catalyzes the synthesis of FLG. The combination of the two is the composition of the skin. Important condition for surface barriers. Therefore, the two can also be used as indicators to evaluate the functional status of the skin barrier.

[0075] The real-time fluorescent quantitative PCR method is used to detect the regulation of the composition on the expression level of the target gene.

[0076] Set up 6 groups of other components on the 6-well plate respectively, the media with different compositions contained, wherein: negative control (not containing composition NT); positive control (containing hyaluronic acid HA of 0.01% mass concentration); In the medium containing the composition obtained in Ex...

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Abstract

The invention provides a composition with barrier repairing and anti-aging effects as well as a preparation method and an application thereof. The composition is prepared from the following components: rosa multiflora flower water, a rosa multiflora flower extract and a hydrolyzed red alga extract in a mass ratio of (10-90): (0.5-3): (1-6). The effect of the composition mixed according to a certain proportion is better than that of a single component with the same concentration. The composition with barrier repairing and anti-aging effects provided by the invention takes internal stability maintenance and external repair as a starting point, and prevents and improves the aging symptoms of the skin from the dimensions of moisturizing, oxidation resistance, brightening, tightening and the like. The composition provided by the invention not only retains the unique fragrance of rosa multiflora, but also realizes the reutilization of rosa multiflora flower residues, and conforms to the concept of greenness, naturalness and sustainability.

Description

technical field [0001] The invention belongs to the field of cosmetics, and in particular relates to a composition with barrier repairing and anti-aging effects, a preparation method and application thereof. Background technique [0002] Modern people are facing multiple pressures, such as fast-paced life, irregular work and rest, environmental pollution, etc., which induce young people aged 20-25 to "decline before they are old" and begin to show signs of aging, such as fine lines, dry lines, dark spots, etc. Heaviness, dark circles, slightly loose contour lines, etc. Improper skin care for young people can also cause premature aging. [0003] Most of the raw materials of anti-aging compositions on the market focus on the repair of damaged skin and treat the symptoms rather than the root cause. As the outermost structure of the human body, the skin is the first barrier for the human body to resist external damage, and its importance is self-evident. The stratum corneum o...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61K8/9717A61Q19/08A61Q19/00
CPCA61K8/9789A61K8/9717A61Q19/08A61Q19/00A61K2800/5922A61K2800/805
Inventor 周秋娜金荣熙周春亚金炫延尹锺赫金延埈
Owner COSMAX CHINA INC
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