Bovine bone collagen peptide and production method thereof

A collagen peptide and production method technology, applied in the fields of peptide preparation, chemical instruments and methods, animal/human proteins, etc., can solve the problems of insufficient extraction rate of bovine bone collagen peptides, insufficient small molecule active polypeptides, etc. The effect of good impurity removal and concentration and low cost

Inactive Publication Date: 2021-04-30
王朝辉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The bovine bone collagen peptide extraction rate obtained by the above me

Method used

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  • Bovine bone collagen peptide and production method thereof

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Comparison scheme
Effect test

Embodiment 1

[0025]Method for producing a bone collagen peptide, including the following steps:

[0026](1) Prior to the treatment of raw materials: In addition to the impurities on the bones, then use the broken bone machine to smash to 2 cm bone blocks, and then cleaned the bones after pulverization, and crush to 50 mesh;

[0027](2) Purification of raw materials: The crushing of bovine bone powder is placed in a 0.1 mol / l NaOH solution of 18 times its mass, shaking at a temperature of 3 ° C for 3 hours, to remove non-collagen substance, after filtering with distilled water After washing the bones, the liquid ratio was placed in a concentration of 10% n-hexane, and the degreasing was shaken at a temperature of 3 ° C for 3 hours, and the fat was filtered. The distilled water was repeatedly washed with a bone-like-neutrality. After leaving the liquid, it was allowed to add 0.25 mol / L ethylenediamine tetra acetate salt concentrated solution, and the temperature was threatened by 3 ° C for 3 hours. ...

Embodiment 2

[0037]Method for producing a bone collagen peptide, including the following steps:

[0038](1) Prior to the treatment of raw materials: In addition to the impurities on the bones, then use the broken bone machine to smash to 2 cm bone blocks, and then cleaned the bones after pulverization, and crush to 50 mesh;

[0039](2) Purification of raw materials: The crushing of bovine bone powder is placed in a 0.1 mol / l NaOH solution of 20 times, and shakes at a temperature of 4 ° C for 3 hours to remove non-collagen substances. After filtration, it is repeated after filtration. After washing the bones, the liquid is placed in a concentration of 10% n-hexane with 1: 7, and the degreas of degreasing is 3 hours at the temperature of 4 ° C, to remove the fat, after filtering The distilled water was repeatedly washed with a bone-like neutrality. After leaving the liquid, it was allowed to add 0.25 mol / L ethylenediamine tetra-different sodium salt concentrated solution to the temperature of 4 ° C ...

Embodiment 3

[0049]Method for producing a bone collagen peptide, including the following steps:

[0050](1) Prior to the treatment of raw materials: In addition to the impurities on the bones, then use the broken bone machine to smash to 2 cm bone blocks, and then cleaned the bones after pulverization, and crush to 50 mesh;

[0051](2) Purification of raw materials: The crushing of bovine bone powder is placed in a 0.1 mol / l NaOH solution of 20 times, and shakes at a temperature of 5 ° C for 3 hours to remove non-collagen substances. After filtration, it is repeated after filtration. After washing the bones, the liquid is placed in a concentration of 10% n-hexane solution after proceeding, and the degreas of degreasing is 3 hours at the temperature of 5 ° C, to remove fat, after filtering Distilled water repeatedly washed the bone-like neutrality, and after proceeding from 1: 10, 0.25 mol / L ethylenediamine tetra acetate salt concentrated solution was added, and the temperature was 3 hours at the t...

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Abstract

The invention belongs to the field of collagen peptides, and relates to a bovine bone collagen peptide and a production method thereof, the production method comprises the following steps: (1) pretreating raw materials; (2) purifying the raw materials; (3) shaking and extracting an acetic acid solution; (4) performing primary enzymolysis; (5) performing secondary enzymolysis; (6) filtering with activated carbon; (7) filtering with an inorganic nanofiltration ceramic membrane; (8) concentrating; and (9) performing spray drying. The collagen peptide is prepared by fully utilizing bovine bone which is a bovine processing byproduct, the cost is low, and the collagen extraction rate is greater than 85%; the prepared yak bone collagen oligopeptide is white or faint yellow powder, the purity is greater than 90%, the molecular weight is less than 1000Da, and the yak bone collagen oligopeptide has no peculiar smell; after bovine bones are prepared into bone meal, non-collagen substances, fat and calcium in bovine bone paste can be basically separated and removed through purification of a NaOH solution, a normal hexane solution and an ethylenediamine tetraacetic acid disodium salt concentrated solution, and the prepared bioactive peptide product is not sticky and basically does not contain impurities such as fat and cholesterol.

Description

Technical field[0001]The present invention belongs to the field of collagen peptides, and in particular, the present invention relates to a bone neutral protein peptide and a production method thereof.Background technique[0002]Collagen is rich in glycine, proline, hydroxyproline, and other amino acids. Collagen is a polymer functional protein that is the main component of the skin, accounting for 80% of the skin leather layer, which constitutes a fine elastic network in the skin, securely locking moisture, supporting the skin. Collagen is a helical fibrous protein, which is screwed by three peptide chains, is also the most abundant protein in the human body, which is widely distributed in connective tissue, skin, bones, internal organs and muscle cavities, ligaments, sclera, etc. It is more than 30% of the total protein of the human body. It is rich in proline, hydroxyproline, etc., which is rich in the human body, is an important part of human cells, especially skin extracellular m...

Claims

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Application Information

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IPC IPC(8): C07K14/78C12P21/06C07K1/34C07K1/14
CPCC07K14/78C12P21/06
Inventor 王朝辉
Owner 王朝辉
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