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Technology for detecting sulfur stable isotope of root tip meristem culture garlic

A stable isotope and meristem technology, which is applied in the analysis of materials, material analysis by electromagnetic means, and measurement devices, can solve the problems of affecting the detection results and difficult to focus on the receiver, achieving good reproducibility, easy operation, The effect of high accuracy

Inactive Publication Date: 2021-04-30
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When switching from carbon and nitrogen isotope detection to sulfur isotope detection, due to the influence of the memory effect of the instrument, SO with a high mass-to-charge ratio 2 + Difficult to effectively focus on the receiver, affecting detection results

Method used

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  • Technology for detecting sulfur stable isotope of root tip meristem culture garlic
  • Technology for detecting sulfur stable isotope of root tip meristem culture garlic
  • Technology for detecting sulfur stable isotope of root tip meristem culture garlic

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A root tip meristem culture garlic sulfur stable isotope analysis technology, comprising the following steps:

[0023] a. After drying, grinding, and filtering the garlic bulb sample of the root tip meristem, accurately weigh 5.0 mg of the sample and put it into a tin cup and wrap it;

[0024] b. IAEA-S-1 (silver sulfide, δS34=-0.30‰), IAEA-S-3 (silver sulfide, δS34=-32.3‰), NBS-127 (barium sulfate, δS34=20.3‰) is the standard product.

[0025] c. Use an element analyzer-stable isotope mass spectrometer to detect the sample to be tested; the parameters of the element analyzer are: oxidation furnace 1000 ° C, chromatographic column 80 ° C, the catalyst in the quartz tube in the oxidation furnace is 25 grams of tungsten oxide and granular pure Copper 50 grams.

[0026] d. The high-energy ion source ionizes the sample to generate SO + ; The ion source parameters of the stable isotope mass spectrometer are: ion source core voltage 5.7KV, ion source lens voltage 163V.

...

Embodiment 2

[0034] A root tip meristem culture garlic sulfur stable isotope analysis technology, comprising the following steps:

[0035] a. The root tip meristem cultured garlic bulb samples were dried, ground, and sieved, and 1.0, 2.5, 4.0, 5.5, 7.0, 8.5, 10.0 mg samples were accurately weighed respectively, put into tin cups and wrapped.

[0036] b. IAEA-S-1 (silver sulfide, δS34=-0.30‰), IAEA-S-3 (silver sulfide, δS34=-32.3‰), NBS-127 (barium sulfate, δS34=20.3‰) is the standard product.

[0037] c. Use an element analyzer-stable isotope mass spectrometer to detect the sample to be tested; the parameters of the element analyzer are: oxidation furnace 1000 ° C, chromatographic column 80 ° C, the catalyst in the quartz tube in the oxidation furnace is 25 grams of tungsten oxide and granular pure Copper 50 grams.

[0038] d. The high-energy ion source ionizes the sample to generate SO + ; The ion source parameters of the stable isotope mass spectrometer are: ion source core voltage 5.7K...

Embodiment 3

[0046] comparative test

[0047]

[0048] In summary, the method of the present invention has the advantages of high precision, good reproducibility, easy operation and maintenance, and high analysis efficiency, and can be applied to the precise detection of sulfur isotopes in garlic samples cultured from root apical meristems.

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Abstract

The invention discloses a technology for detecting sulfur stable isotope of root tip meristem culture garlic in an SO+ mode, which comprises the following specific steps: drying, grinding and filtering a root tip meristem culture garlic bulb sample by using a screen, filling into a tin cup, wrapping, and detecting a sample to be detected by using an element analyzer-stable isotope mass spectrometer. According to the method, secondary ionization and efficient separation of high-energy electron flow to SO2+ are realized by utilizing parameters of the tuning ion source ion lens and the quadrupole mass analyzer, so that the requirement of rapid and accurate detection of the sulfur isotope ratio of the root apex meristem culture garlic is perfected, and the detection result is high in accuracy, good in reproducibility and easy to operate and maintain.

Description

[0001] The invention was funded by the 2021 Agricultural Achievement Transformation and Promotion Project of the Tianjin Agricultural Commission. technical field [0002] The invention belongs to the technical field of stable isotope analysis, and in particular relates to a method for detecting garlic sulfur stable isotope. Background technique [0003] Garlic is an important spicy vegetable variety on the table of our people, and has important edible and medicinal values. A number of studies have confirmed that a variety of biologically active substances contained in garlic, such as allicin and polysaccharides, have significant anti-virus, anti-cancer cell activity, anti-thrombosis, lowering blood lipids, lowering blood pressure, and alleviating atherosclerosis. [0004] Plant tissue culture refers to the cultivation of isolated plant organs, tissues, cells or protoplasts under sterile environment conditions, artificially controlling temperature, light, humidity, nutrition,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 尚云涛王振英刘晓颖高健范宝莉李海燕
Owner TIANJIN NORMAL UNIVERSITY
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