The invention provides a method for determining oil sources by using sulfur isotopes in monomer sulfur-containing compounds of crude oil. The method comprises the following steps: crude oil is dissolved in ethanol, dichloromethane or dichloroethane, an alkylating reagent is added, and filtrate is obtained after a reaction; ethanol, dichloromethane or dichloroethane are removed from the filtrate, and oily substances are obtained; the oily substances and an alkane solvent of C5-C7 are mixed, and separation is carried out for deposition; the deposition is dissolved in dichloromethane, trichloromethane or acetonitrile, a first dealkylating reagent is added, and a solution A is obtained; extraction is carried out, and a thiophene compound and a solution B are obtained; a second dealkylating reagent is added into the solution B for carrying out a second dealkylation reaction, and a solution C is obtained; extraction is carried out, and a thioether compound is obtained; sulfur isotopic distribution of monomers in the thiophene compound and the thioether compound as well as sulfur isotopic distribution of sulfate in an oil-generating rock stratum system are determined; two sulfur isotopic distributions are compared, and the stratum whose isotopic distribution is most close to is the oil source layer.