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Microgel assembly scaffold for tissue regeneration and repair, and preparation method thereof

A tissue regeneration and microgel technology, applied in the direction of prosthesis, pharmaceutical formulation, pharmaceutical science, etc., can solve the problems of weak interaction force, poor reproducibility, large surgical wound, etc., to avoid displacement and mild reaction conditions. Effect

Active Publication Date: 2021-06-04
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical bond assembly scaffolds are very stable, generally do not have injectable properties, and the surgical wound is large during implantation; physical bond assembly scaffold microgels have weak interaction, strong fluidity, and poor mechanical properties; cell-cell interaction The microgel assembly time of the action-assembled scaffold is long and there are many influencing factors, resulting in poor reproducibility; the external force-assembled scaffold is difficult to maintain its shape after the external force is removed

Method used

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  • Microgel assembly scaffold for tissue regeneration and repair, and preparation method thereof
  • Microgel assembly scaffold for tissue regeneration and repair, and preparation method thereof
  • Microgel assembly scaffold for tissue regeneration and repair, and preparation method thereof

Examples

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preparation example Construction

[0039] The preparation of the hyaluronic acid modified by the carbon-carbon double bond used below comprises the following steps:

[0040] Weigh 2g of hyaluronic acid with a molecular weight of 100,000 and dissolve it in 200ml of deionized water at 40°C. After returning to room temperature, 4ml of methacrylic anhydride was added, and the pH was stabilized at 8 with 1mol / L NaOH solution. After 2 hours of reaction, it was stirred overnight at 4°C. The solution was dialyzed with deionized water for 3 days by using a dialysis bag with a molecular weight of 8000-12000, and then freeze-dried to obtain hyaluronic acid modified by a carbon-carbon double bond.

[0041] The preparation of the hyaluronic acid modified by dopamine used below comprises the following steps:

[0042] (1) Take 21.34g of 2-(N-morpholine)ethanesulfonic acid solid and dissolve it in 1L of deionized water to obtain MES buffer;

[0043] (2) Weigh 1 g of hyaluronic acid with a molecular weight of 100,000 and dissol...

Embodiment 1

[0055] (1) Add 30mg of carbon-carbon double bond and phenylboronic acid double-modified gelatin and 50mg of carbon-carbon double bond-modified hyaluronic acid into 2ml of PBS solution, heat up to 40°C and stir to dissolve, then add 0.5mol / L NaOH to adjust The pH is 7, after stirring, add 5 mg of photoinitiator phenyl-2,4,6-trimethylbenzoyl lithium phosphonate (Lap), and stir to obtain the aqueous dispersed phase;

[0056] (2) Dilute the 2% Fluo-Surf 7500 solution ten times with fluorinated oil HFE7500 to a concentration of 0.2wt%, as the oil phase continuous phase;

[0057] (3) The two phases are introduced into the T-shaped microfluidic chip. Adjust and control the flow rate of the dispersed phase to be 0.4ml / h, 1ml / h and 1.6ml / h respectively, and the flow rate of the stationary continuous phase to be 6ml / h, and obtain three kinds of microemulsion droplets with different sizes and diameters of water-in-oil by shearing. The prepared droplets were collected in 0.2wt% fluorinat...

Embodiment 2

[0062] (1) Add 40mg of hyaluronic acid modified with carbon-carbon double bonds and phenylboronic acid, and 40mg of collagen modified with carbon-carbon double bonds into 2ml of PBS solution, heat up to 40°C and stir to dissolve, then add 0.5mol / L NaOH to adjust the pH 7, after stirring, add 3 mg of photoinitiator phenyl-2,4,6-trimethylbenzoyl lithium phosphonate (Lap), and then mix with the cells to make an aqueous dispersion with a cell density of 1.5 million / mL ;

[0063] (2) Dilute 2wt% fluorinated oil HFE7500 with fluorinated oil ten times to a concentration of 0.2wt%, as the oil phase continuous phase;

[0064] (3) The two phases are introduced into the cross-shaped microfluidic chip. Among them, the flow rates of the dispersed phase and the continuous phase are adjusted and controlled to be 0.5ml / h and 4ml / h respectively, and water-in-oil single emulsion droplets are obtained by shearing. The prepared droplets were collected in 0.2wt% fluorinated oil HFE7500, and then...

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Abstract

The invention discloses a microgel assembly scaffold for tissue regeneration and repair, and a preparation method thereof. The scaffold has injectability and can be used for minimally invasive treatment of tissue defects and promotion of tissue regeneration and repair. The preparation method of the microgel assembly scaffold comprises the following steps: respectively synthesizing a carbon-carbon double bond and phenylboronic acid dual-modified polymer material and a carbon-carbon double bond modified polymer material; preparing microgel liquid drops by adopting a microfluidic technology, and initiating free radical polymerization by blue light or ultraviolet light to realize internal crosslinking of microgel; and preparing dopamine modified polymer molecules, and assembling the microgel into the scaffold by utilizing a dynamic bonding effect constructed by phenylboronic acid and dopamine. The scaffold has porosity, self-healing property and cell compatibility, can be injected at physiological temperature, is easy to adhere to surrounding tissues, and shows a good tissue regeneration and repair effect.

Description

technical field [0001] The invention belongs to the technical fields of biomedical materials, tissue engineering and regenerative medicine, and in particular relates to a microgel assembly bracket for tissue regeneration and repair and a preparation method thereof. [0002] technical background [0003] Every year, millions of patients suffer from lesions or injuries of their own tissues. Autologous or allogeneic tissue transplantation can help patients with tissue defects to a certain extent, but the shortage of donor sources and long-term immune rejection after transplantation largely limit the application of this technology. Tissue engineering combines active factors, cells and scaffold materials to prepare engineered tissues for the treatment of tissue defects, which has broad application prospects. Hydrogels have good mechanical properties and biocompatibility, and are a large class of scaffold materials commonly used in tissue engineering. However, traditional hydroge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/50A61L27/52A61L27/56
CPCA61L27/26A61L27/50A61L27/52A61L27/56A61L2300/412A61L2400/06C08L5/08C08L89/00
Inventor 曹晓东董华冯琦李定果
Owner SOUTH CHINA UNIV OF TECH
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