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Polymer sponge microsphere and preparation method thereof

A polymer and sponge technology, applied in pharmaceutical formulations, drug delivery, medical science, etc., can solve the problems of difficulty in obtaining uniform size, polymer sponge microspheres are difficult to remove toxicity, time-consuming, etc., and achieve the ability to promote cell proliferation. , good adhesion and proliferation characteristics, efficient preparation method

Pending Publication Date: 2022-03-22
安徽医科大学附属口腔医院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a polymer sponge microsphere and its preparation method, which solves the problem that it is difficult to obtain controllable microspheres with uniform size and high porosity in the traditional way, and the operation is complicated and time-consuming. In addition, the cross-linking process is chemical cross-linking, and the cross-linking agents used are all highly toxic chemical reagents. It is difficult to completely remove the toxicity of the obtained polymer sponge microspheres by washing.

Method used

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  • Polymer sponge microsphere and preparation method thereof
  • Polymer sponge microsphere and preparation method thereof
  • Polymer sponge microsphere and preparation method thereof

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preparation example Construction

[0030] Such as Figure 1-7 As shown, the present invention provides a kind of technical scheme: a kind of polymer sponge microsphere and preparation method thereof, this polymer sponge microsphere and preparation method thereof comprise the following steps:

[0031] S1. The emulsified polymer droplets are frozen in situ by the freezing liquid, the size of the polymer droplets is 100-1000 microns, and the polymer is one of chitosan, silk protein, collagen, sodium alginate and gelatin or several, the mass fraction of the polymer solution is 0.20-5%, based on the weight of pure water, preferably 0.5%, 1% and 2%, the example is 1%, the solution frozen in situ is toluene, xylene, One or two kinds of solutions such as chloroform;

[0032] S2, followed by mixing with the fixative solution and performing physical crosslinking, the fixative contains sodium hydroxide of Alcohol solution, alcohol solution of calcium chloride, alcohol solution of potassium hydroxide, alcohol solution of...

Embodiment 1

[0035] Embodiment 1: the preparation of porous chitosan sponge microsphere

[0036] Weigh 1g of chitosan solution into 100ml of 1% acetic acid aqueous solution to prepare a 1% chitosan solution with a mass fraction, and draw 8ml of prepared chitosan solution using a 10ml syringe. Directly connected to the inner tube (inner diameter of 60 microns) on the microfluidic device, and set the extrusion speed to 0.008ml / min, use a 50ml syringe to extract 40ml of 3% (V / V) Span-80 cyclohexane solution As the extrusion phase, it was connected to the outer tube (with an inner diameter of 310 μm) on the microfluidic device, and the extrusion speed was set to 0.4 ml / min. The extruded droplets are frozen into solid microspheres in toluene pre-cooled by liquid nitrogen (-40°C ~ -20°C), and 0.1mol / L NaOH ethanol solution is prepared as a solution containing a fixative, and the fixative is pre- Cool to -10°C, mix and cross-link the obtained frozen solid chitosan microspheres with a fixative so...

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Abstract

The invention relates to the technical field of biological tissue engineering, and discloses a polymer sponge microsphere and a preparation method thereof.The preparation method of the polymer sponge microsphere comprises the following steps that S1, emulsified polymer liquid drops are subjected to in-situ freezing through freezing liquid; s2, mixing with a solution of a fixing agent, and carrying out physical crosslinking; s3, recovering the room temperature, and unfreezing the emulsion to obtain polymer sponge microspheres; the preparation method disclosed by the invention is efficient and energy-saving, the obtained polymer sponge microspheres have uniform size and pore, shape memory and injectable performance, a cross-linking agent for chemical cross-linking with cytotoxicity is not introduced in the preparation process by adopting physical cross-linking, and a freeze-drying process is also omitted; the surface and the interior of the polymer sponge microsphere have relatively good porosity and pore traffic, so that the polymer sponge microsphere shows good adhesion proliferation characteristics and cell proliferation promoting performance in an in-vitro cell culture test.

Description

technical field [0001] The invention relates to the technical field of biological tissue engineering, in particular to a polymer sponge microsphere and a preparation method thereof. Background technique [0002] Biological tissue provides a three-dimensional growth environment for cells through the network structure of extracellular matrix nanofibers, and affects cell adhesion, proliferation and differentiation. The classic tissue engineering technique (top-down method) is to inoculate seed cells on prefabricated porous biodegradable polymer scaffold materials, which provide space support for the development of new tissue, when used for larger bone tissue repair During reconstruction, often due to insufficient supply of oxygen and nutrients and accumulation of metabolic waste, the physiological process of cells is affected and apoptosis is prone to occur, thus affecting the repair effect of tissue defects. The recently proposed bottom-up method based on microcell carriers c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/28C08L5/08A61L27/20A61L27/50A61L27/56
CPCC08J9/28A61L27/20A61L27/50A61L27/56C08J2305/08A61L2400/16A61L2400/06C08L5/08
Inventor 王默涵何家才杨璐张静怡丛运红顾哲源李贝贝
Owner 安徽医科大学附属口腔医院
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