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Preparation and application of injectable bone repair material capable of activating endogenous TGF beta1 in situ

An injection type, bone repair technology, applied in the direction of prosthesis, drug delivery, tissue regeneration, etc., can solve problems affecting the process of bone repair

Active Publication Date: 2022-04-22
PEKING UNIV SCHOOL OF STOMATOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the non-physiological pH environment can also significantly affect the process of bone repair

Method used

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  • Preparation and application of injectable bone repair material capable of activating endogenous TGF beta1 in situ
  • Preparation and application of injectable bone repair material capable of activating endogenous TGF beta1 in situ
  • Preparation and application of injectable bone repair material capable of activating endogenous TGF beta1 in situ

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Example 1 Preparation of Injectable Bone Repair Material That Can Activate Endogenous TGFβ1 in Situ

[0039] The hydrogel material is divided into two parts: matrix and gelatin microspheres. The gelatin microspheres are prepared in a microfluidic manner. In order to ensure the injectability of the final hydrogel material, the diameter of the prepared gelatin microspheres needs to be controlled at about 120 μm.

[0040] (1) Preparation of Type A gelatin microspheres: The core component of the microfluidic system is the microfluidic chip. The chip is designed with SolidworksCAD software and then CNC-cut. It is made of PMMA resin with a thickness of 3 mm and an internal channel width of 150 μm. The water phase was prepared with 5% Type A gelatin solution, the oil phase was mineral oil containing 20wt% surfactant Span80, and the injection speeds of the oil phase and the water phase were 18.8ul / min and 3.08ul / min, respectively. The collection device was placed in a water ...

Embodiment 2

[0045] Example 2 Application of Injectable Bone Repair Materials That Can Activate Endogenous TGFβ1 in Situ

[0046] Firstly, a SD rat skull defect model was constructed. Anesthetized by intraperitoneal injection of 2% pentobarbital sodium, fixed the head of the rat, prepared the skin, disinfected with 2% povidone iodine, cut the skin and muscular layer 5-8mm in the middle of the cranial suture, and peeled off the periosteum to expose the bone surface. A circular bone defect was made on both sides of the cranial suture with a trephine drill with an outer diameter of 5 mm, and pH7 PEI-modified gelatin-based microsphere hydrogel (pH7 Mgel) and pH10 PEI-modified gelatin-based microsphere hydrogel were injected into the defect area (ph10 Mgel), the muscle layer was sutured with 5-0 absorbable suture, and the skin layer was sutured with 5-0 silk suture. After the operation, the rats were placed on a 37°C heat preservation pad until the rats regained consciousness, and they were fe...

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Abstract

An injectable bone repair material capable of activating endogenous TGF beta1 in situ is formed by compounding a water-containing matrix and microspheres, the microspheres are TypeA gelatin microspheres which are prepared by adopting a microfluidic method and have uniform particle sizes, and the water-containing matrix is formed by dispersing laponite XLG and polyethyleneimine (PEI) modified gelatin molecules into a water phase. Alkaline bicarbonate or neutral phosphate is adsorbed into gelatin microspheres by a diffusion loading method, and then the gelatin microspheres are mixed with a water-containing matrix to prepare the injectable bone repair material.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to the preparation and application of an injectable bone repair material capable of activating endogenous TGFβ1 in situ. Background technique [0002] A long-term goal of oral and maxillofacial regenerative medicine is to treat oral and maxillofacial bone tissue defects and loss of function due to trauma, tumor resection, periodontal disease, alveolar ridge resorption, treatment-related osteonecrosis of the jaw, and congenital malformations and aesthetic deformities. When the injury exceeds the critical bone defect size, the bone tissue cannot heal spontaneously. Currently, autologous bone grafting remains the "gold standard" for bone defect repair. However, the risks of pain, infection, nerve damage, and loss of function associated with bone harvesting have limited its application. Allogeneic bone or xenogeneic bone transplantation is often accompanied by problems such as imm...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/02A61L27/50A61L27/58
CPCA61L27/222A61L27/025A61L27/50A61L27/58A61L2400/06A61L2430/02C08L89/00
Inventor 周永胜牛宇霆杨振杨洋
Owner PEKING UNIV SCHOOL OF STOMATOLOGY
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