Trichoderma longibrachiatum and application thereof
A technology of Trichoderma longibrachia and engineering bacteria, which is applied in the direction of application, fungicides, fungi, etc., can solve problems such as time-consuming and labor-intensive pollution, environmental pollution, and millet pollution, and achieve good application prospects
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Embodiment 1
[0042] Example 1 Separation and Identification of Long branches of Moth T23
[0043] 1. Preparation of medium
[0044] (1) PDA culture medium configuration:
[0045] 200 grams of geofwheat slice, boil 800 ml of distilled water, boiling about 20 minutes after boiling, the yarn is filtered, and 20 g of glucose is added, and the mixing is mixed and allowed to adjust to 1 liter, add 15 grams of agar powder, and heat melting to the triangle Bottle, after the sealing of 121 ° C for 20 minutes in a high pressure pot.
[0046] (2) PDB culture medium:
[0047] 200 grams of geofwheat slice, boil 800 ml of distilled water, boiling about 20 minutes after boiling, filtered through the gauze, add 20 grams of glucose, stir well, uniformly add water to 1 liters, dispensed into the triangular bottle, 121 ° C in a high pressure pan after the sealing Sterilize for 20 minutes.
[0048] (3) LB solid medium: 10 grams of trypsin in a liter culture medium, 10 grams of sodium chloride, 5 grams of yeast ex...
Embodiment 2
[0056] Example 2 Long branched Moth T23 inhibited Valley Terracia Growth and Reissue Rabbit
[0057] The long branus T23 and the respective supply strain were activated on the PDA plate culture medium. When the colony would be long, the diameter of 5 mm was taken with a pore, and the Trichoderma T23 germacterial plate and Each of the test val val pathogenic fungi inoculated at both ends of the culture dish, between 6 cm, each of the test valley pathogens Alone in PDA plate as a control, start recording growth rate from the second day of vaccination until the control bacteria lost a petri dish, experiment Repeated 3 times, see Figure 4 - Figure 8 It can be seen that there is a different degree of inhibition of the T23 strains of the Pubus and Pecusa T23. Figure 4 It is cultured for the confrontation of Long Branch T23 and the Critumacteria, A: Comparison, B: Texas of Music Music Musica on the Critum Fungi; Figure 5 For the confrontation of Long branus T23 and Suqua Pleids, A: Contr...
Embodiment 3
[0063] Example 3 Long branches T23 fermentation liquid inhibited the growth of ceremonic bacteria
[0064] Coquin T23, Coyoma Pamptology and Cyttospecatroplasses were cultured at 28 ° C for 5 days on solid PDA culture medium, and the surface of the petri dish was flushed with spores with sterilized distilled water. The concentration of 1 × 10 6 A / mL spore suspension. Glutellaria grows on sorghum culture medium, eluzes sorghum preparation concentration is 1 × 10 6 A / mL spore suspension. The long branched xercosporina suspension was inoculated into the PDB liquid medium in a PDB liquid medium, and the filtrate was collected after 28 ° C 150 rpm / min, and the filament was removed by the double gauze, and the impurities were removed by filter paper. After bacteria filtration, mixed with equal volume valley disease, milf spots and milf curlosis, with PDB solutions, respectively, etc., respectively, etc., respectively, etc., Suqua Pamptology and Corcinate bacteria The liquid is mix...
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