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Trichoderma longibrachiatum and application thereof

A technology of Trichoderma longibrachia and engineering bacteria, which is applied in the direction of application, fungicides, fungi, etc., can solve problems such as time-consuming and labor-intensive pollution, environmental pollution, and millet pollution, and achieve good application prospects

Active Publication Date: 2021-06-11
GRAIN RES INST HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the onset, it will pollute the millet and affect its variety and safety
There are many physiological races in many diseases of millet, such as cereal blast and rust, which are prone to variation in physiological races, resulting in the loss of disease resistance of millet, and the varieties bred in production have a lack of resistant varieties
The spraying of chemical pesticides on millet is likely to cause environmental pollution and pesticide residues, which is not conducive to the green production of millet
For a long time, the prevention and treatment of millet diseases has mainly relied on frequent spraying of pesticides, which is not only time-consuming and labor-intensive, but also pollutes the environment, causes pesticide residues and produces drug resistance, which does not meet the trend of people's demand for green food

Method used

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  • Trichoderma longibrachiatum and application thereof
  • Trichoderma longibrachiatum and application thereof
  • Trichoderma longibrachiatum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Separation and Identification of Long branches of Moth T23

[0043] 1. Preparation of medium

[0044] (1) PDA culture medium configuration:

[0045] 200 grams of geofwheat slice, boil 800 ml of distilled water, boiling about 20 minutes after boiling, the yarn is filtered, and 20 g of glucose is added, and the mixing is mixed and allowed to adjust to 1 liter, add 15 grams of agar powder, and heat melting to the triangle Bottle, after the sealing of 121 ° C for 20 minutes in a high pressure pot.

[0046] (2) PDB culture medium:

[0047] 200 grams of geofwheat slice, boil 800 ml of distilled water, boiling about 20 minutes after boiling, filtered through the gauze, add 20 grams of glucose, stir well, uniformly add water to 1 liters, dispensed into the triangular bottle, 121 ° C in a high pressure pan after the sealing Sterilize for 20 minutes.

[0048] (3) LB solid medium: 10 grams of trypsin in a liter culture medium, 10 grams of sodium chloride, 5 grams of yeast ex...

Embodiment 2

[0056] Example 2 Long branched Moth T23 inhibited Valley Terracia Growth and Reissue Rabbit

[0057] The long branus T23 and the respective supply strain were activated on the PDA plate culture medium. When the colony would be long, the diameter of 5 mm was taken with a pore, and the Trichoderma T23 germacterial plate and Each of the test val val pathogenic fungi inoculated at both ends of the culture dish, between 6 cm, each of the test valley pathogens Alone in PDA plate as a control, start recording growth rate from the second day of vaccination until the control bacteria lost a petri dish, experiment Repeated 3 times, see Figure 4 - Figure 8 It can be seen that there is a different degree of inhibition of the T23 strains of the Pubus and Pecusa T23. Figure 4 It is cultured for the confrontation of Long Branch T23 and the Critumacteria, A: Comparison, B: Texas of Music Music Musica on the Critum Fungi; Figure 5 For the confrontation of Long branus T23 and Suqua Pleids, A: Contr...

Embodiment 3

[0063] Example 3 Long branches T23 fermentation liquid inhibited the growth of ceremonic bacteria

[0064] Coquin T23, Coyoma Pamptology and Cyttospecatroplasses were cultured at 28 ° C for 5 days on solid PDA culture medium, and the surface of the petri dish was flushed with spores with sterilized distilled water. The concentration of 1 × 10 6 A / mL spore suspension. Glutellaria grows on sorghum culture medium, eluzes sorghum preparation concentration is 1 × 10 6 A / mL spore suspension. The long branched xercosporina suspension was inoculated into the PDB liquid medium in a PDB liquid medium, and the filtrate was collected after 28 ° C 150 rpm / min, and the filament was removed by the double gauze, and the impurities were removed by filter paper. After bacteria filtration, mixed with equal volume valley disease, milf spots and milf curlosis, with PDB solutions, respectively, etc., respectively, etc., respectively, etc., Suqua Pamptology and Corcinate bacteria The liquid is mix...

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PUM

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Abstract

The invention discloses trichoderma longibrachiatum and application thereof, and belongs to the field of biological control of plant diseases. The trichoderma longibrachiatum is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 25, 2021, and the preservation number is CGMCC NO.21474. The trichoderma longibrachiatum T23 strain is obtained in the separation process of rhizoctonia solani, is parasitized on a rhizoctonia solani colony, has the characteristics of fast growth, more spores and the like, and is suitable for large-scale production. Trichoderma longibrachiatum T23 aerial hyphae are not developed, bacterial colonies are white at the beginning, the bacterial colonies become green at the later stage along with growth of the hyphae, and the trichoderma longibrachiatum and rhizoctonia solani are cultured oppositely, so that the growth of the bacterial colonies of the rhizoctonia solani can be inhibited, the bacterial colonies of the rhizoctonia solani can be completely covered at the later stage, and the rhizoctonia solani can be parasitized in a winding manner. The strain has a good application prospect in the green disease prevention and control direction of millets.

Description

Technical field [0001] The invention belongs to the field of plant disease biological control, and in particular, the present invention relates to a long branched wood mold and its application thereof. Background technique [0002] Valley originated in my country, its planting area and production accounted for more than 80% of the world. Xiaomi is rich in nutrients, which is increasingly favored in the world's small grains. Valley drought resistance, is a high-quality drought-drought crop in the arid area in northern my country, plus grain is the high-quality forage of cattle and sheep. In the case of cultivated land, it is the first choice for the development of livestock farming industry. It can be seen that Valley has shown increasing strength in the current planting structure adjustment, and the development prospect is unlimited. There are a variety of diseases in different parts of the valley, and the main disease of the leaves is a variety of crute, grain, and millet bendel...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/38A01P3/00C12R1/885
CPCC12N1/14A01N63/38
Inventor 李志勇白辉董志平张梦雅刘佳马继芳王永芳全建章刘磊
Owner GRAIN RES INST HEBEI ACAD OF AGRI & FORESTRY SCI
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