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Application of colla corii asini in preparation of health-care product for promoting improvement of skin collagen

A collagen and health care product technology, which can be used in applications, skin diseases, medical preparations containing active ingredients, etc., and can solve the problems of restricting the promotion and application of macromolecular protein health products.

Pending Publication Date: 2021-06-22
SHAN DONG DONG E E JIAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Limit the promotion and application of macromolecular protein health products represented by donkey-hide gelatin

Method used

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  • Application of colla corii asini in preparation of health-care product for promoting improvement of skin collagen
  • Application of colla corii asini in preparation of health-care product for promoting improvement of skin collagen
  • Application of colla corii asini in preparation of health-care product for promoting improvement of skin collagen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] In vivo experimental invention of donkey-hide gelatin supplementing skin collagen layer

[0033] 1 material

[0034] 1.1 Experimental animals

[0035] The BALB / C mice used in the present invention were all purchased from Beijing Weitong Lihua Experimental Animal Co., Ltd., half male and half female, weighing 20g±2g. Experimental animal license number: SCXK (Beijing) 2016-0006.

[0036] 1.2 Health products

[0037] Donkey-hide gelatin powder (Dong'e donkey-hide gelatin Co., Ltd.)

[0038] 1.3 Reagents

[0039] Reagents related to HE staining were purchased from Jiubai, and MASSON staining kits were purchased from Beijing Suolaibao Technology Co., Ltd. (Cat. No. G1345)

[0040] 1.4 Main Instruments

[0041] Two-photon confocal microscope (Olympus, MPE FV1000), Abrio liquid crystal polarizing imaging system (USA, CRI), upright fluorescence microscope (Olympus, BX51), tissue sectioning system (Thermo)

[0042] 2 methods

[0043] 2.1 Animal modeling

[0044] Normal ...

Embodiment 2

[0073] In vitro invention of donkey-hide gelatin promoting fibroblast proliferation

[0074] 1 material

[0075] 1.1 Cell lines

[0076] HFF-1 was purchased from Xiehe Cell Bank

[0077] 1.2 Health products

[0078] Donkey-hide gelatin powder (Dong'e donkey-hide gelatin Co., Ltd.)

[0079] 1.3 Reagents

[0080] CCK8 was purchased from Dongren Chemical Technology (Shanghai) Co., Ltd. (Cat. No.: CK04), rabbit anti-mouse Ki67 was purchased from Abcam (Cat. No.: ab15580), and other conventional immunofluorescence reagents were purchased from Beyond Biotechnology Co., Ltd.

[0081] 1.4 Main Instruments

[0082] Laser confocal scanning microscope (Olympus, FV1000); inverted phase contrast microscope (Olympus, BX43); microplate reader (BioTeK, Synergy H1); ultra-clean workbench (Suzhou Antai Air Technology Co., Ltd., SW-CJ-JFD)

[0083] 2 methods

[0084] 2.1 Cell culture

[0085] Take the frozen HFF-1 cells in liquid nitrogen and resuscitate them in warm water at 37-40°C, th...

Embodiment 3

[0098] Invention of Collagen Typing for Supplementing the Collagen Layer of the Skin by Oral Ejiao

[0099] 1 material

[0100] 1.1 Reagents

[0101] Anti-Collagen I antibody (ab34710) and Anti-Collagen III antibody (ab6310) were purchased from Abcam; other routine immunofluorescence reagents were purchased from Beyotime Biotechnology Co., Ltd.

[0102] 1.2 Instruments

[0103] Laser confocal microscope (Olympus, FV1000), cryostat (Thermo)

[0104] 2 methods

[0105] 2.1 Immunofluorescence

[0106] Take living tissue about 1×0.5cm in size, put it into a cryostat and embed it in a cryostat after quick freezing, slice thickness 10μm, add 4% paraformaldehyde after drying at room temperature, fix for 30min, wash twice with PBS, and place in 0.5% TX-100 at room temperature Permeabilize for 20 minutes, add 5% BSA and incubate at 37°C for 1 hour, add 1:600 ​​diluted primary antibody and incubate overnight at 4°C. After washing with PBS for 2 days, add 1:1000 diluted fluorescent...

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PUM

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Abstract

The invention relates to application of colla corii asini in preparation of a health-care product for promoting improvement of skin collagen, and belongs to the technical field of preparation of health-care products. The health care product prepared from the colla corii asini can improve the density and orderliness of skin collagen of young people, can also improve the orderliness of skin collagen of old people, and can improve the density and orderliness of skin collagen of people with qi deficiency and blood deficiency; fibroblast proliferation can also be promoted; the growth of I-type collagenous fibers of people with thinned skin thickness is promoted; the growth of early-stage type III collagenous fibers and the growth of later-stage type I collagenous fibers of people with no significant change in skin thickness are promoted.

Description

technical field [0001] The invention relates to the technical field of preparation of health care products, in particular to the application of donkey-hide gelatin in the preparation of health care products for improving skin collagen. Background technique [0002] Donkey-hide gelatin is a famous traditional nourishing medicine in my country. It is known as the "holy medicine for nourishing blood". It is sweet in taste and flat in nature. Earlier inventions showed that the main components of donkey-hide gelatin are collagen, polypeptides, amino acids and so on. Its chemical composition is mostly composed of collagen and its partial hydrolyzate, with a nitrogen content of 16.43% to 16.54%. Long-term clinical practice has shown that donkey-hide gelatin has therapeutic and regulatory effects on the blood system, immune system and cardiovascular system. At present, many women eat donkey-hide gelatin for beauty, thinking that it has great value for skin nutrition and health car...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/00A61K35/36A61P17/00
CPCA23L33/00A61K35/36A61P17/00A23V2002/00A23V2200/318
Inventor 刘海滨顾建军任汝静高登峰刘维岩张淹孙阳恩王春艳
Owner SHAN DONG DONG E E JIAO
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