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An antibacterial nano-droplet and its application

A nano and droplet technology, applied in the nano field to achieve good bacteriostatic effect and good destructive effect

Active Publication Date: 2021-11-23
GUANGDONG PHARMA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Chinese patent CN 111841599 A discloses a carbon quantum dot composite material (nitrogen-doped carbon quantum dot N-CQDs) with photocatalytic antibacterial properties, which is easy to operate, easy to control and low in preparation cost, although the prepared nitrogen-doped Carbon quantum dots have high photocatalytic antibacterial activity against Escherichia coli and Staphylococcus aureus, but there is no report on the effect of carbon quantum dots on biofilms
There are no reports on fluorescent nanodroplets with their own antibacterial properties

Method used

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  • An antibacterial nano-droplet and its application
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  • An antibacterial nano-droplet and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 A method for preparing liquid-liquid phase separation nano-droplets based on surfactants and sulfur-doped carbon quantum dots

[0043] 1. Take PKOO and S-CQDs to prepare a mixed solution, the concentration of PKOO in the mixed solution is 34mM, and the concentration of S-CQDs is 2mg / mL. Among them, PKOO was purchased from Shanghai Yincong New Material Co., Ltd.; S-CQDs were synthesized by the following method: Weigh 5 g of citric acid and 0.8 g of mercaptoethylamine, add 40 mL of pure water, stir to dissolve, and place in 50 mL of polytetrafluoroethylene In the lining, heat at 150°C for 150min, after the reaction, dialyze for 48h, and freeze-dry to obtain S-CQDs.

[0044] 2. After the mixed solution described in step 1 was placed at room temperature for 24 hours, the solution changed from turbid to clear, centrifuged at 10,000 rpm for 30 minutes, discarded the supernatant, and obtained a colloidal solid.

[0045] 3. Dissolve the colloidal solid obtained in st...

Embodiment 2

[0046] Example 2 Detection of the inhibitory ability of nano-droplets to Staphylococcus aureus

[0047] Adopt the nano droplet that obtains in embodiment 1 to carry out following experiment:

[0048] 1. Preparation of lecithin-Tween 80 nutrient agar culture plate

[0049] Lecithin-Tween 80 nutrient agar medium (purchased from Guangdong Huankai Microbial Technology Co., Ltd., item number 1095521) was sterilized by autoclaving for 30 minutes. After cooling to a certain temperature, pour the melted and cooled nutrient agar into the bacteria-specific medium once. Pour about 15mL of nutrient agar into each 9mm bacterial petri dish and solidify for later use. Note that the temperature of the poured petri dish should not be too high, otherwise there will be water vapor around the petri dish, and the plate will easily cause experimental errors.

[0050] 2. Add 3mL PBS buffer solution to the slant of Staphylococcus aureus, then take out the bacterial solution and dilute it to OD with ...

Embodiment 3

[0052] Example 3 Detection of Inhibitory Ability of Nano-droplets to Escherichia coli

[0053] Referring to the experimental method of Example 2, the inhibitory ability of the nano-droplets to Escherichia coli was detected, and the concentration of the nano-droplets was set to . 5, 10, 15, 20, 25, 30, 50 μg / mL.

[0054] The inhibition curve of nano-droplets against Escherichia coli is shown in figure 2 .

[0055] After curve fitting, the minimum inhibitory concentration MIC value of nano-droplets against Escherichia coli was finally obtained as 21.38 μg / mL.

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Abstract

The invention provides an antibacterial nano droplet and its application in inhibiting bacterial biofilm. The preparation method of the antibacterial nano-droplets is as follows: place the mixed solution of oleic acid amidopropyl dimethyl tertiary amine and sulfur-doped carbon quantum dots at room temperature, centrifuge, and discard the supernatant to obtain liquid liquid Phase-separated condensed phase, dispersed in aqueous solution. The nano-droplet provided by the invention has better antibacterial effect on Staphylococcus aureus and Escherichia coli. The destruction rate of 100μg / mL nano-droplets on Staphylococcus aureus biofilm can reach 82.68%, and it has a strong destruction effect on the biofilm formed by Staphylococcus aureus.

Description

technical field [0001] The invention belongs to the field of nanotechnology, and in particular relates to an antibacterial nano-droplet based on a surfactant and carbon quantum dots and its application in inhibiting bacterial biofilm. Background technique [0002] Infection with pathogenic bacteria (such as Escherichia coli, Staphylococcus epidermidis, and Staphylococcus aureus) is the main cause of death in immunocompromised patients. For such diseases, antibacterial drugs are mostly used for prevention and treatment clinically. On the one hand, the excessive use of antibacterial drugs can easily lead to drug resistance; on the other hand, pathogenic bacteria can easily form biofilms, which will not only lead to enhanced drug resistance of bacteria, but also interfere with the identification and killing of the patient's immune system mechanism. The research and development of new antibacterial biofilm materials, which can destroy the growth of biofilm, is an important cont...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N59/02A01N59/00A01N25/30A01P1/00
CPCA01N25/30A01N59/00A01N59/02
Inventor 刘意郑茵李睿春伍春娴张威
Owner GUANGDONG PHARMA UNIV