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Application of nitrogen-doped graphdiyne quantum dots in chloramphenicol detection

A technology of alkyne quantum and nitrogen doping, applied in the field of bioelectrical analysis and detection, can solve the problems of poor stability, high cost, and reduced advantages

Pending Publication Date: 2021-07-06
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the graphene material only contains graphitic carbon, which is a kind of sp 2 Hybridized carbon (sp 2 -C), and the general detection effect of graphene materials is mediocre, and the advantages of noble metal materials in sensing are greatly reduced due to their high cost and poor stability

Method used

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  • Application of nitrogen-doped graphdiyne quantum dots in chloramphenicol detection
  • Application of nitrogen-doped graphdiyne quantum dots in chloramphenicol detection
  • Application of nitrogen-doped graphdiyne quantum dots in chloramphenicol detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Preparation of N-GDYQDs and preparation of electrode material dispersant solution

[0042] 1. Preparation of N-GDYQDs

[0043] 0.25mg / mL graphyne aqueous solution (GDY) was ultrasonically treated for 3h, and the ultrasonic power was 400W to obtain a small-sized fragmented GDY dark brown solution; ) mixed (volume ratio 2:1) ultrasonic treatment for 30 minutes, ultrasonic power 400W, transferred to the reaction kettle 180 ℃ water heating for 8 hours to obtain a uniform yellow solution, and then dialyzed the gained yellow solution (the molecular weight cut-off of the dialysis bag is 500Da) 3 day, change the water every 8 hours, freeze-dry to get N-GDYQDs, attached figure 1 Its electron microscope picture shows that the nitrogen-doped graphyne quantum dot N-GDYQDs has a particle size of 2-5 nm.

[0044] 2. Preparation of N-GDYQDs electrode material dispersant solution

[0045] Add 750 μL of deionized water and 200 μL of absolute ethanol solution to 2 mg of N-GDYQDs, soni...

Embodiment 2

[0048] Comparison of electrochemical performance of different electrochemical sensing materials

[0049] The electrocatalysis of 500 μmol / L chloramphenicol standard solution based on different electrode materials (glassy carbon (GC), graphene (Graphene), graphyne (GDY), nitrogen-doped graphyne quantum dots) was tested by cyclic voltammetry performance. Various electrode materials were made into dispersants with a concentration of 2 mg / mL (the preparation method of the dispersant was the same as that of the dispersant in Example 1), and 6 μL of different electrode material dispersants were drip-coated on the same glassy carbon working electrode successively. (clean the electrode after each use), and form a three-electrode system with a platinum wire electrode and an Ag / AgCl electrode to form an electrochemical sensor. In the potential range of 0.5~-0.9V, the sweep speed is 50mV / s, the amplitude is 0.05V, and the pulse width is 0.05s. The electrochemical performance of the stan...

Embodiment 3

[0051] Evaluation of Electrochemical Performance of N-GDYQDs

[0052] The electrochemical performance of the electrochemical sensor based on N-GDYQDs modification was tested by electrochemical workstation CHI760, and the electrochemical sensing signal was tested by differential pulse voltammetry.

[0053] Take 6 μL of the N-GDYQDs electrode material dispersant prepared in Example 1 and drop-coat it on a glassy carbon electrode with a diameter of 0.5 mm. After drying at room temperature, use it as a working electrode modified with N-GDYQDs. The / AgCl electrode forms a three-electrode system electrochemical sensor, and the concentration of measuring pH is about 7 and is the DPV electrochemical signal of the phosphate buffer solution (prepared in embodiment 1) of 5000 μ mol / L chloramphenicol, and the DPV test potential range is-0.2~ -1V, the amplitude is 0.05V, and the pulse width is 0.05s. It can be found that when chloramphenicol is added to the solution, the DPV test has an o...

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Abstract

The invention discloses application of nitrogen-doped graphdiyne quantum dots in chloramphenicol detection. A method for detecting chloramphenicol by using an electrochemical sensor based on a nitrogen-doped graphdiyne quantum dot material comprises the following steps: preparing dispersion liquid, preparing chloramphenicol solutions with different concentrations, and performing electrochemical detection by using an N-GDYQDs sensor; establishing a differential pulse volt-ampere curve of chloramphenicol detected by N-GDYQDs; and detecting the concentration of chloramphenicol in the sample to be detected. The linear range of the response to chloramphenicol based on the nitrogen-doped graphdiyne quantum dot material is 0.1-1000 [mu] mol / L and 1500-5000 [mu] mol / L, and the detection limit is 0.018 [mu] mol / L. The detection method disclosed by the invention has the advantages of simplicity and convenience in operation, low cost, high sensitivity, low detection limit and high response speed; not only can efficient and sensitive detection of chloramphenicol be realized, but also quantitative analysis of chloramphenicol can be realized.

Description

technical field [0001] The invention belongs to the technical field of bioelectrical analysis and detection, and relates to a detection method for chloramphenicol, in particular to the application of a nitrogen-doped graphyne quantum dot in the detection of chloramphenicol. Background technique [0002] Chloramphenicol (2,2-dichloro-n-[1,3-dihydroxy-1-(4-nitrophenyl)propane2-yl]acetamide) is a broad-spectrum Effective on both gram-positive and gram-negative bacteria. Therefore, due to its broad antibacterial spectrum and relatively low cost, it is used to treat human and animal diseases. The mechanism of action of chloramphenicol is generally bacteriostatic, it is bactericidal at high concentrations, or it is bactericidal when used against Streptococcus pneumoniae, Neisseria meningitidis or Haemophilus influenzae. Chloramphenicol is effective both parenterally and orally (80% bioavailability) and has excellent tissue penetration. It reaches 30%–50% of serum concentrations...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/30C09K11/65B82Y20/00B82Y40/00
CPCG01N27/308G01N27/48C09K11/65C09K11/0883B82Y20/00B82Y40/00Y02A50/30
Inventor 蔡称心谷青雨胡耀娟乔玲潘永慧吴萍
Owner NANJING NORMAL UNIVERSITY
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