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Method for preparing Vaccinium dunalianum extract through microwave synergistic composite enzymatic hydrolysis

A compound enzymatic hydrolysis and extraction technology, which is applied in the direction of sugar derivatives, drug combinations, pharmaceutical formulations, etc., can solve the problems of not fully meeting the needs of use, low yield of active ingredients, long extraction time, etc., to achieve excellent supply Effects of hydrogen capacity, few steps, and easy operation

Inactive Publication Date: 2021-07-20
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The existing commonly used preparation method of camphor leaf extract is mainly water extraction method, for example, CN101297692B mainly adopts boiling water decoction and macroporous adsorption resin adsorption to combine to prepare camphor leaf extract, and CN111040006B mainly adopts boiling water extraction Combined with organic solvent extraction to prepare bilberry extract of camphor leaves, these two methods extract and obtain active ingredients to a certain extent, but their extraction rate is low, the extraction time is long, and the yield of active ingredients is low. Can not fully meet the needs of use

Method used

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  • Method for preparing Vaccinium dunalianum extract through microwave synergistic composite enzymatic hydrolysis
  • Method for preparing Vaccinium dunalianum extract through microwave synergistic composite enzymatic hydrolysis
  • Method for preparing Vaccinium dunalianum extract through microwave synergistic composite enzymatic hydrolysis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Raw material pretreatment

[0031] The leaf buds were collected by Judy Orange. After naturally drying, it was pulverized to 60 mesh, and the powder sample was pretreated after the ultraviolet lamp was prepared, and the dry grinding bottle was loaded, and the spare was stored at 4 ° C.

[0032] 2. Extraction and refining of active ingredients

[0033] Precisely referred to in the pre-treatment of the sediment 5.0000g added to the microwave extraction bottle, the liquid ratio (g / mL) is added to 1: 30 to add deionized water as extracting solvent, add sample mass 2% composite enzyme (fiber) Surrsenase: pectin enzyme = 2: 1), the microwave extraction power is 600 W, and the extraction temperature is 50 ° C in the constant temperature water bath. After the end of the enzymatic solution, the enzyme was oxidase 10 min in hot water at 75 ° C. After cooling, filtrate, the filtrate was concentrated under reduced pressure, dried to constant weight to the constant weight, and the bro...

Embodiment 2

[0035]Raw material pretreatment with Example 1, precisely referred to in the pre-treatment of the Upperburder powder 5.0000g added to the microwave extraction bottle, and the liquid ratio (g / mL) is added to the addition of deionized water as extraction solvent, add Sample mass 2% compound enzyme (cellulase: pectin enzyme = 1: 0), microwave extraction power is 500 W, and the extraction temperature is 40 ° C in a constant temperature water bath in a constant temperature water bath. After the end of the enzymatic solution, the enzyme was oxidase 10 min in hot water at 75 ° C. After cooling, filtrate, the filtrate was concentrated under reduced pressure, dried to the constant weight to the constant weight, and the brown-yellow amorphous solid powder, the extraction rate was 18%, and the product was recrystallized by methanol, light yellow The needle crystals, the purity is ≥90%, and the yield is 90%. Among them, the methanol recrystallization is purified to: prepare the extract to f...

Embodiment 3

[0037] Raw material pretreatment with Example 1, accurately referred to in the pre-treatment of the suspended orange leaf bud powder 5.0000g added to the microwave extraction bottle, the liquid ratio (g / mL) 1: 50 is added to the extracting solvent, add Sample mass 2% complex enzyme (cellulase: pectin enzyme = 0: 1), microwave extraction power is 400 W, and the extraction temperature is 45 ° C constant temperature water bath enzymatic solution 2.5 h. After the end of the enzymatic solution, the enzyme was oxidase 10 min in hot water at 75 ° C. After cooling, filtrate, the filtrate was concentrated under reduced pressure, and the mixture was dried at 80 ° C to the crude extract of the Yulipin orange, the brown-yellow amorphous solid powder, the extraction rate was 17%, and the product was recrystallized by methanol, pale yellow Needle crystals, purity ≥ 90%, yield is 88%. Among them, the methanol recrystallization is purified to: prepare the extract to form a hot saturated saturat...

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Abstract

The invention discloses a method for preparing a Vaccinium dunalianum extract through microwave synergistic composite enzymatic hydrolysis, which comprises the following steps: collecting leaf buds of Vaccinium dunalianum, naturally drying, and crushing into powder; adding the powder into a microwave extraction bottle, adding water as an extraction solvent according to a material-liquid ratio of 1: (10-60), adding a compound enzyme the mass of which is not more than 5% of the mass of the sample, and carrying out enzymolysis for 0.5-2.5 hours under the conditions that the microwave extraction power is 100-800W and the extraction temperature is 30-60 DEG C; after the enzymolysis is finished, inactivating enzyme, carrying out suction filtration, concentrating filtrate under reduced pressure, and drying to obtain a Vaccinium dunalianum crude extract. The method is easy to operate, has few steps, is safe and non-toxic, and is a green and environment-friendly process. Experiments prove that the content of effective components in the extract is high, the extract shows excellent hydrogen supply capacity, ABTS+. Free radical scavenging capacity, HO. free radical scavenging capacity and reduction capacity, and the antioxidant activity is higher than that of a common water extraction method; acute toxicity tests show that the product is safe and reliable.

Description

Technical field [0001] The present invention belongs to the technical field of plant extract, and more particularly to a method of microwave synergistic complex enzyme decryption. Background technique [0002] The rapid development of science and technology has brought convenience to human life and also bring potential threats to human health. Radiation, including ionizing radiation (X-rays, gamma rays, etc.) and non-ionizing radiation (infrared, ultraviolet rays, microwaves, lasers, etc.) have become fourth largest contamination after continuous water, atmospheric, and noise. Oxidative stress occurs when organisms are suffered from harmful radiation stimuli, and various active free radicals such as superior oxygen, hydroxyl radicals are produced in vivo. Free radicals are atoms, molecules or ions containing one or more asymmetric electrons, and is necessary to maintain normal life activities. There are a variety of free radical clearance routes in the normal body, free radical p...

Claims

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Application Information

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IPC IPC(8): A61K36/45A61P39/06A61P17/18A61P17/16A61P39/00A61K127/00C07H15/203C07C69/732
CPCA61K36/45A61P39/06A61P17/18A61P17/16A61P39/00A61K2236/17A61K2236/15A61K2236/331A61K2236/19A61K2236/53A61K2236/51C07H15/203C07C69/732
Inventor 杨晓琴赵平王琪雷悦朱国磊韩康佳谢东
Owner SOUTHWEST FORESTRY UNIVERSITY
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