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Pseudomonas for preventing and treating radix pseudostellariae diseases as well as separation, screening and identification method and application

A technique for separation and screening of Pseudomonas, applied in the field of plant disease prevention and control, can solve the problems such as the decline of yield and quality of Radix Radix japonica medicinal materials, strong acidification of soil, frequent occurrence of diseases, etc.

Active Publication Date: 2021-07-23
贵州中医药大学
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of artificial cultivation, there are serious continuous cropping obstacles in heterophylla heterophylla, mainly manifested by frequent occurrence of diseases. Common diseases include blight, leaf spot, root rot, etc., and the incidence rate increases year by year. Continuous cropping for 2 years is about 30% less than regular cropping, and continuous cropping for more than 3 years is more than 50% lower than regular cropping, resulting in a decline in the yield and quality of Pseudostellaria heterophylla, and causing great economic losses to farmers.
During the prevention and control of Pseudostellaria Pseudostellariae disease, currently ginseng farmers mainly use chemical agents such as propamocarb hydrochloride, fluopicolide, and deltamethrin to prevent and control diseases, but excessive use of chemicals will lead to ecological balance damage, Adverse effects such as increased pesticide residues, increased resistance of pathogenic bacteria, and strong acidification of the soil

Method used

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  • Pseudomonas for preventing and treating radix pseudostellariae diseases as well as separation, screening and identification method and application
  • Pseudomonas for preventing and treating radix pseudostellariae diseases as well as separation, screening and identification method and application
  • Pseudomonas for preventing and treating radix pseudostellariae diseases as well as separation, screening and identification method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1 Pseudomonas Isolation and Screening Method for Preventing and Controlling Radix Pseudostellariae Diseases

[0067] 1) collect fresh Radix Pseudostellariae leaves and tubers and rinse with water;

[0068] 2) Cut into pieces with sterilized scissors and place them in a mortar;

[0069] 3) Add 10 mL of PBS solution to 1 g of leaf and root tissue, collect the suspension after ultrasonication for 20 min, and dilute to 10 -2 , to obtain the bacteria-containing dilution;

[0070] 4) take 10 -2 100 μL of the diluted bacterium-containing dilution was spread on the PDA plate, and each treatment was repeated three times;

[0071] 5) After culturing at 28°C for 2 days, pick single colonies with different phenotypes and streak them in LB medium for purification;

[0072] 6) identification of the isolated bacterial strain Pseudomonas;

[0073] 7) Pseudomonas was screened by the plate confrontation culture method, Fusarium, Alternaria, Botrytis fuckers, and Rhizopus ory...

Embodiment 2

[0077] Example 2 Pseudomonas Isolation and Screening Method for Preventing and Controlling Radix Pseudostellariae Diseases

[0078] 1) collect fresh Radix Pseudostellariae leaves and tubers and rinse with water;

[0079] 2) Cut into pieces with sterilized scissors and place them in a mortar;

[0080] 3) Add 10mL of PBS solution according to 0.5g of leaf and root tuber tissue, collect the suspension after ultrasonication for 15min, and dilute to 10 -2 , to obtain the bacteria-containing dilution;

[0081] 4) take 10 -2 100 μL of the diluted bacterium-containing dilution was spread on the PDA plate, and each treatment was repeated three times;

[0082] 5) After culturing at 25°C for 1 day, pick single colonies with different phenotypes and streak them in LB medium for purification;

[0083] 6) identification of the isolated bacterial strain Pseudomonas;

[0084] 7) Pseudomonas was screened by the plate confrontation culture method, Fusarium, Alternaria, Botrytis fuckers, an...

Embodiment 3

[0088] Example 3 Pseudomonas Isolation and Screening Method for Preventing and Controlling Pseudomonas Pseudostellariae Diseases

[0089] 1) collect fresh Radix Pseudostellariae leaves and tubers and rinse with water;

[0090] 2) Cut into pieces with sterilized scissors and place them in a mortar;

[0091] 3) Add 10mL of PBS solution to 1.5g of leaf and root tuber tissue, collect the suspension after ultrasonication for 25min, and dilute to 10 times in turn with a volume ratio of 10 times. -2 , to obtain the bacteria-containing dilution;

[0092] 4) take 10 -2 100 μL of the diluted bacterium-containing dilution was spread on the PDA plate, and each treatment was repeated three times;

[0093] 5) After culturing at 30°C for 3 days, pick single colonies with different phenotypes and streak them on LB medium for purification;

[0094] 6) identification of the isolated bacterial strain Pseudomonas;

[0095] 7) Pseudomonas was screened by the plate confrontation culture method...

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Abstract

The invention relates to pseudomonas for preventing and treating radix pseudostellariae diseases as well as a separation, screening and identification method and application. According to the research, the pseudomonas is separated from a radix pseudostellariae plant, has relatively strong stress resistance, is non-toxic and harmless to human and livestock, and is environment-friendly. Meanwhile, the pseudomonas has an obvious inhibiting effect on various pathogenic bacteria such as leaf spot, damping off, root rot and purple root rot of the pseudostellaria heterophylla, and can be used as a biocontrol bactericide for preventing and treating various fungal diseases of the pseudostellaria heterophylla.

Description

technical field [0001] The invention relates to the technical field of plant disease prevention and control, in particular to a Pseudomonas for disease prevention and control, a separation, screening and identification method and application thereof. Background technique [0002] Pseudostellaria heterophylla (Miq.) Pax ex Paxet Hoffm. is the dry tuber root of Pseudostellaria heterophylla (Miq.) Pax ex Paxet Hoffm., which has the effects of nourishing qi and invigorating the spleen, promoting body fluid and moisturizing the lungs. Modern pharmacological studies have shown that Pseudostellariae Pseudostellariae has the effects of lowering blood sugar, improving chronic heart failure, and antioxidative activity. In the process of artificial cultivation, there are serious continuous cropping obstacles in heterophylla heterophylla, mainly manifested by frequent occurrence of diseases. Common diseases include blight, leaf spot, root rot, etc., and the incidence rate increases year...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12Q1/04C12Q1/689C12N15/11A01N63/27A01P3/00C12R1/38
CPCC12N1/20C12N1/02C12Q1/689C12Q1/04A01N63/27Y02A50/30
Inventor 袁青松周涛江维克王小爱汪露欧小宏肖承鸿
Owner 贵州中医药大学
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