Method for detecting propyl gallate and formaldehyde

A technology of propyl gallate and formaldehyde, which is applied in the field of chemical analysis and detection to achieve the effects of rapid reaction, consistent detection results and reliable detection

Pending Publication Date: 2021-07-23
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the excellent catalytic activity of the single-atom nanozyme, the present invention has synthesized a zinc-based single-atom nanozyme, using PG as the enzyme reaction substrate, and performing PG colorimetric detect

Method used

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  • Method for detecting propyl gallate and formaldehyde
  • Method for detecting propyl gallate and formaldehyde
  • Method for detecting propyl gallate and formaldehyde

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: Determination of formaldehyde in PG and dried white fungus in soybean oil sample

[0027] 1. Preparation of zinc-based single-atom nanozyme Zn-N-C: Stir and mix 0.7g zinc acetate, 20mL methanol and 50g potassium chloride, place in a vacuum drying oven, dry at 80°C for 24h, mix the dried solid with 0.4g After mixing 2-methylimidazole and 20mL methanol, place in a vacuum oven, dry at 80°C for 48h to remove methanol, place the obtained solid particles in a tube furnace, and calcinate at 750°C for 2h under the protection of nitrogen, and wash the obtained solid with pure water Wash to obtain small black particles insoluble in water, namely Zn-N-C;

[0028] 2. Preparation of the working curve of propyl gallate: add 0.1mg / mL zinc-based single-atom nanozyme (Zn-N-C) 100µL, 50mmol / L H 2 o 2 100µL standard solution of propyl gallate with a concentration in the range of 1.25~200mg / kg, dilute to the mark with citric acid-disodium hydrogen phosphate buffer solution ...

Embodiment 2

[0042] Example 2: Determination of formaldehyde in PG and chicken feet in blended oil samples

[0043] 1, Zn-N-C preparation: with embodiment 1;

[0044] 2, the making of propyl gallate working curve: with embodiment 1;

[0045] 3, formaldehyde working curve making: with embodiment 1;

[0046] 4. Determination of PG in blend oil samples

[0047] (1) Sample treatment: Accurately weigh 2g of blended oil into a 25mL colorimetric tube, add 7mL of ethanol solution with a volume concentration of 95%, vortex for 1min to mix well, let it stand for a while, and heat it in a water bath at 85°C for 15s to promote its separation. layer; transfer the supernatant to the concentration bottle with a straw, then repeat the extraction twice with 7mL 95% ethanol solution, combine the extracts and dilute to 25mL to obtain the sample solution to be tested;

[0048] (2) Sample determination: Same as in Example 1, the content of PG in the blended oil sample was 11.21 mg / kg.

[0049] 5. Determina...

Embodiment 3

[0052] Embodiment 3: Determination of PG in cookies and formaldehyde in shredded bread

[0053] 1. Preparation of Zn-N-C: Stir and mix 1g of zinc acetate, 20mL of methanol and 50g of potassium chloride, place it at 80°C for 30h in vacuum, and mix the dried solid with 0.3g of 2-methylimidazole and 23mL of methanol After that, place it at 80°C for 30 hours to remove methanol, place the obtained solid particles in a tube furnace, and calcinate at 800°C for 2 hours under the protection of nitrogen, and wash the obtained solids with pure water to obtain small black particles that are insoluble in water. It is zinc-based single-atom nanozyme Zn-N-C;

[0054] 2, making of propyl gallate working curve: with embodiment 1.

[0055] 3, formaldehyde working curve making: with embodiment 1;

[0056] 4. Determination of PG in cookies

[0057] (1) Sample treatment: Weigh 5.00g of evenly powdered biscuit sample into a 100mL Erlenmeyer flask, add 8mL of absolute ethanol, vortex mix for 1min...

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Abstract

The invention discloses a method for detecting propyl gallate and formaldehyde, which is characterized in that a zinc-based monatomic nano-enzyme is synthesized by a salt template method, based on the characteristic that Zn-N-C zinc has electron and geometric structures similar to those of the active center of natural metalloenzyme, and the strong simulated peroxidase activity is shown. The substrate propyl gallate can be rapidly oxidized into a yellow oxidized quinones substance, and formaldehyde can inhibit the oxidation reaction, so that the rapid detection method of PG and formaldehyde is established, and the method is applied to detection and analysis of PG and formaldehyde in food. The result of the method is consistent with a method for determining nine antioxidants in food in GB5009.32-2016 food safety national standard and a method for determining formaldehyde in food in GB/T5009.49; the method can only selectively and rapidly oxidize PG, the reaction speed of other antioxidants is low, and the method has the advantages of being high in sensitivity, high in specificity, easy to operate, rapid and the like.

Description

technical field [0001] The invention relates to the technical field of chemical analysis and detection, in particular to a method for simultaneously detecting propyl gallate and formaldehyde. Background technique [0002] Propyl gallate (PG), also known as propyl gallate, is an antioxidant commonly used in the food industry. my country's GB 2760-2014 "National Food Safety Standard Food Additives Use Standard" stipulates that PG is used in the maximum amount of oily food It is 0.1 g / kg (based on the content in oil). Studies have shown that excessive use of PG can cause harm to human health, such as kidney damage or contact dermatitis. Common detection methods for PG include colorimetry, liquid chromatography, gas chromatography, and liquid chromatography-mass spectrometry. Colorimetry is often used because it is simple, fast, and does not require large-scale equipment. However, due to poor characteristics, The detection interference is serious, which becomes a difficult prob...

Claims

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Application Information

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IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 杨德志李秋兰陈子昭杨亚玲
Owner KUNMING UNIV OF SCI & TECH
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