Application of konjac polysaccharide degradation products KGM-1k and KGM-5k in preparation of probiotic protective agent

A konjac polysaccharide and degradation product technology is applied in the biological field to achieve the effects of improving survival rate, improving biological efficacy and enhancing stress resistance

Active Publication Date: 2021-08-06
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether the degradation products of konjac polysaccharides have protective effects on probiotics has not been reported

Method used

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  • Application of konjac polysaccharide degradation products KGM-1k and KGM-5k in preparation of probiotic protective agent
  • Application of konjac polysaccharide degradation products KGM-1k and KGM-5k in preparation of probiotic protective agent
  • Application of konjac polysaccharide degradation products KGM-1k and KGM-5k in preparation of probiotic protective agent

Examples

Experimental program
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Effect test

Embodiment 1

[0054] This example studies the effect of KGM poly sugar degradation product KGM-1K on the survival of long bifidobacteria SQ011.

[0055] The simulated gastric mixture of KGM-1K with Konjac polysaccharide degradation products is prepared: first formulated MRS medium containing no carbon source, the pH of the MRS medium containing no carbon source is adjusted to 2 with hydrochloric acid, and 5% (kgm- Konjac polysaccharide degradation product KGM-1K was fully dissolved after 1k / mRS medium, m / m), which was fully dissolved and sterilized at 121 ° C. The gastric protease is then dissolved in an appropriate amount of PBS solution (phosphate buffer solution) and filtered and sterilized; finally, the treated gastric protease is added to the above-mentioned MRS medium containing Konjac polysaccharide degradation product KGM-1K. .

[0056] The simulated small intestinal fluid of KGM-1K with Konjac polysaccharide degradation product is prepared: first adjusting the pH of the MRS medium ...

Embodiment 2

[0071] This example studies the effect of Konjac polysaccharide degradation product KGM-5K on the survival of the long bifidobacterium SQ011.

[0072] The simulated gastric mixture of KGM-5K with Konjac polysaccharide degradation product was prepared: first formulated MRS medium containing no carbon source, the pH of the medium was adjusted to 2 with hydrochloric acid, 5% (KGM-5K / MRS medium) , M / m) konjac polysaccharide degradation product KGM-5K, high pressure sterilization at 121 ° C after dissolving. The gastric protease was then dissolved in an appropriate amount of PBS solution and filtered and sterilized. Finally, the treated gastric protease was added to the MRS medium containing the Konjac polysaccharide degradation product KGM-5K in the concentration of 3 g / L.

[0073] The simulated small intestinal fluid containing Konjac polysaccharide degradation product is prepared: first adjusting the pH of the MRS medium containing free carbon hydroxide to 7, add 3% (Boxer sal...

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Abstract

The invention relates to application of konjac polysaccharide degradation products KGM-1k and KGM-5k in preparation of a probiotic protective agent. The application comprises the following steps of: firstly, destroying the hydrogen-bond interaction among konjac polysaccharide molecules by adopting hydrogen peroxide, then degrading the konjac polysaccharide molecules by utilizing cellulase, and carrying out alcohol precipitation, separation and purification by 60% ethanol to prepare the konjac polysaccharide degradation products KGM-1k and KGM-5k components with uniform molecular weight. The application has the beneficial effects that the influence of the konjac polysaccharide degradation products KGM-1k and KGM-5k on the survival condition of probiotics under the storage conditions of simulated gastric juice, small intestinal juice, vacuum freeze drying and different storage temperatures (4 DEG C and 25 DEG C) is investigated for the first time; and results show that the konjac polysaccharide degradation products KGM-1k and KGM-5k can enhance the stress resistance of the probiotics, increase the viable count of the probiotics under the storage conditions of vacuum freeze drying and different storage temperatures and increase the survival rate of the probiotics in the digestive tract, so that the gastrointestinal fluid can be tolerated to enter the predetermined part of the intestinal tract to realize field planting, and the application has important significance for improving the biological effects of the probiotics.

Description

Technical field [0001] The present invention belongs to the use of biological technology, and specific to konjac polysaccharide degradation products KGM-1K and KGM-5K in the preparation of benefit of the bacteria protective agent. Background technique [0002] Probiotics refers to the human health, especially the health of the living system, and has the growth of harmful bacteria, protecting the gastric mucosa, improving the body's immunity, preventing diarrhea, maintaining the intestinal function, promoting gastric peristalsis, Help digestion and throughput. When the intestinal flora is disordered, the repairing flora structure can be adjusted by oral pricing. However, before these probiotics reach the colorectal planting, they first need to pass through the stomach and small intestines, while the gastric fluids and gastric proteases in the stomach and the trypsin and choline salt substances in the small intestine may destroy the physiological activity of the bacteria, resulting...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04C12P19/14C12P19/00C12R1/125C12R1/01C12R1/225C12R1/145
CPCC12N1/20C12N1/04C12P19/14C12P19/00Y02A50/30
Inventor 王仲孚龚桂萍黄琳娟方洁王雪
Owner NORTHWEST UNIV(CN)
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