Oriented microchannel stent for promoting tissue regeneration and preparation method of stent

A tissue regeneration and microchannel technology, which can be used in tissue regeneration, pharmaceutical formulations, prostheses, etc., and can solve the problems of difficult to control pore size, random structure of porous protein scaffolds, and limitations.

Inactive Publication Date: 2021-08-13
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although each of these strategies has its own advantages and ease of scaffold fabrication, these techniques are limited in their ability to fabricate complex tissue structures, lacking precision and controllability
All in all, the porous protein scaffolds prepared by these techniques are random in structure, difficult to control the pore size, and lack of topology-guided structure.

Method used

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  • Oriented microchannel stent for promoting tissue regeneration and preparation method of stent
  • Oriented microchannel stent for promoting tissue regeneration and preparation method of stent
  • Oriented microchannel stent for promoting tissue regeneration and preparation method of stent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of Nanoscale Porous Silk Fibroin Scaffolds by Electrospinning Using PCL as a Template

[0033] Preparation of PCL nanofiber scaffold: Weigh 2.5 grams of PCL with a molecular weight of 80,000, add it to 10 ml of dichloromethane, stir and dissolve at room temperature until clear, and prepare a PCL solution with a concentration fraction of 25% (m / v). Electrospinning was performed at room temperature with a relative humidity of 60%. Cover the electrospinning drum receiving device with aluminum foil and ground it, put 25% (m / v) PCL spinning solution into a disposable syringe with a diameter of about 14.95 mm, and connect the high voltage DC power supply to the syringe needle. Adjust the needle of the syringe to the center of the cylindrical receiver, set the distance between the needle and the receiver to be 12 cm, the flow rate of the solution to be 2 ml / h, the DC voltage to be 16 kV, and the spinning time to be 40 min. After the preparation is completed, the o...

Embodiment 2

[0037] Fabrication of micron-sized porous chitosan scaffolds by melt spinning using PLGA as a template

[0038] Preparation of PLA crude fiber scaffold: the scaffold was prepared at room temperature by melt spinning. 20 grams of PLA was added to the barrel. Raise the temperature of the barrel to 100°C for 5 hours, adjust the distance between the needle of the barrel and the receiving rod, set the advancing speed of the barrel to 2ml / h, and the moving speed of the x-axis and y-axis of the receiving plate to 1mm / s. It is 15cm, and the spinning time is 60min.

[0039] Preparation of chitosan solution: 10 g of chitosan was weighed and dissolved in 50 ml of acetic acid solution to obtain a 2% chitosan solution.

[0040] Preparation of porous chitosan scaffold: Immerse the PLA fiber template scaffold into the chitosan solution. After the solution is fully immersed in the scaffold, it can be frozen at -20°C, -80°C or liquid nitrogen respectively. After completion, the samples were...

Embodiment 3

[0042] Fabrication of micron-sized porous sodium alginate scaffolds by wet spinning using PLCL as a template

[0043] Preparation of PLCL microfiber scaffold: The scaffold was prepared in a fume hood at room temperature by wet spinning. Weigh 1.5g of PLCL and dissolve it in 10ml of tetrahydrofuran, stir overnight at room temperature to dissolve, and prepare a PLCL solution with a concentration fraction of 15% (mass / volume). Connect a cylindrical receiving rod with a diameter of 2 cm to a rotating motor. Inhale the PLCL spinning solution into the syringe, set the distance between the syringe needle and the receiving rod immersed in the ethanol coagulation bath to be 1cm, the spinning speed to be 2ml / h, the rotating speed of the receiving rod to be 500rpm, and the spinning time to be 30min. After the preparation is completed, the micron fiber scaffold is vacuum-dried for future use.

[0044] Preparation of sodium alginate solution: 10 g of chitosan was weighed and dissolved in...

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Abstract

The invention belongs to the technical field of preparation of porous natural polymer scaffolds in the field of tissue engineering, and relates to a preparation method of an oriented micro-channel scaffold for promoting tissue regeneration. The method comprises the following steps: preparing a fiber support skeleton, filling a natural scaffold material, and eluting the fiber support skeleton. The preparation method has the beneficial effects that the pore diameter of the natural polymer stent is controllable, and the pores are communicating, and the problem that the pore diameter of a natural polymer stent material is difficult to control is successfully solved.

Description

technical field [0001] The invention belongs to the technical field of porous natural polymer scaffold preparation in the field of tissue engineering, and relates to an orientation microchannel scaffold for promoting tissue regeneration and a preparation method thereof. Background technique [0002] Tissue engineering scaffold materials need to simulate the structure and function of extracellular matrix, play a certain role of support and template, provide a place for cells to adhere, grow, differentiate and proliferate, and then guide the regeneration of damaged tissues, thereby repairing damaged tissues and organs. The material properties of scaffolds are thus crucial in determining cell response and fate. An ideal tissue engineering scaffold should meet the following conditions: good biocompatibility; controllable pore structure; high porosity and connectivity; suitable mechanical strength; degradability; active. Three-dimensional tissue engineering scaffold materials u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/50A61L27/18A61L27/34
CPCA61L27/56A61L27/50A61L27/18A61L27/34A61L27/507A61L2400/12A61L2430/20A61L2430/22A61L2430/30A61L2430/10A61L2430/32C08L67/04C08L89/00C08L5/08C08L5/04
Inventor 朱美峰孔德领李雯
Owner NANKAI UNIV
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